Bifunctional Polysulfone-Chitosan Composite Hollow Fiber Membrane for Bioartificial Liver

Abstract

Hollow fiber membranes are widely used as assist devices for bioartificial liver application. Asymmetric porous polysulfone and polysulfone-tocopheryl polyethylene glycol succinate (Psf-TPGS) composite hollow fiber and flat membranes were prepared by phase inversion procedure and subsequently surface modified with chitosan using sulfonation with concentrated sulfuric acid. Sulfonation induces negative charge on the prepared membrane surface and facilitates the attachment of chitosan amine groups by electrostatic interaction. The surface modification of membrane is stable at room temperature as dictated by presence of nitrogen in XPS analysis and amide linkages in FT-IR spectra. Further, biological studies of the membranes were performed using HepG2 cell line. Chitosan is biocompatible and shows structural similarity to glycosaminoglycans, a native liver ECM component. The chitosan-modified composite Psf and Psf-TPGS membranes have shown enhanced attachment and proliferation of HepG2 cells on outer surface as confirmed by the cell counting, DNA content, confocal microscopy, and SEM micrographs. The cells form a 3D multicellular spheroid structure on the chitosan-modified membranes in significantly larger number as seen in the SEM micrographs. Also, the hemocompatibility of the modified composite membranes were comparable to the unmodified membranes. Thus, the chitosan-modified composite membranes we have developed are bifunctional and have the potential to be used in bioartifical liver application.

Advanced Biomaterials and Processing Methods for Liver Regeneration: State‐of‐the‐Art and Future Trends

“…Also, Teotia et al prepared composite hollow fibers from asymmetric porous polysulfone and polysulfone‐tocopheryl polyethylene glycol succinate (Psf‐TPGS). This material was subsequently modified by surface treating with sulfonation followed by addition of chitosan via electrostatic interaction.…”

A non-classical route of efficient plant uptake verified with fluorescent nanoparticles and root adhesion forces investigated using AFM

“…Here, the Cu 2-X Se NPs are synthesized in oleylamine (CS@OA) as reported before, which gives them hydrophobicity. To have an equivalent hydrophilic particle, the as-prepared Cu 2-X Se NPs were coated with amphiphilic biopolymer chitosan (CS@CH) and transferred into hydrophilic 41 – 43 . Synthesizing and stabilizing NPs in solution without capping agent is nearly impossible, which avoids question compare with naked NPs 44 .…”

Hollow fibre membrane bioreactor functionalized with GO‐cryogel 3D matrix promotes liver cell anchoring and their functional maintenance

“…Several methods and materials have been adopted to enhance cell attachment over the HFM surface (Vasanthan et al, 2012). In one study, polysulfone (Psf) HFMs were chemically modified with chitosan to increase cell attachment and functionality (Teotia et al, 2015). In another study, glutaraldehyde‐cross‐linked gelatin (gel)‐coated polyethersulphone (PES) HFMs were used to show the enhanced functions of liver cells (Verma, Modi, Singh, Teotia, Kadam, et al, 2018).…”