Bifunctional folic-conjugated aspartic-modified Fe<sub>3</sub>O<sub>4</sub> nanocarriers for efficient targeted anticancer drug delivery

Khalil, Munawar; Haq, Ely Arina; Dwiranti, Astari; Prasedya, Eka Sunarwidhi; Kitamoto, Yoshitaka

doi:10.1039/d1ra08776b

Cited by 9 publications

(5 citation statements)

References 62 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Additionally, tumor growth and volume were significantly reduced compared to free PTX. Folic acid was also included in aspartic acid-modified magnetite nanoparticles for DOX loading and potential application against skin cancer cell lines (B16-F1) [95]. The aspartic acid, a highly biocompatible molecule, contains two carboxylic acid groups facilitating DOX and Fe 3 O 4 surface conjugation and an amine group for FA anchoring.…”

Section: Treatment Of Other Cancersmentioning

confidence: 99%

“…Fluorescence microscopy, both conventional and confocal, is one of such methods, chosen for its relatively straightforward experimental setup. Its utilization was identified in numerous original papers referenced in this review [46][47][48]50,51,63,71,73,80,84,88,90,95,[101][102][103][104]110,117]. It allows for studying extensive cell populations or relatively sizable tissue samples.…”

Section: Monitoring Of Magnetite-based Nanocarriers Interacting With ...mentioning

confidence: 99%

See 1 more Smart Citation

Recent Progress and Challenges Regarding Magnetite-Based Nanoparticles for Targeted Drug Delivery

Kurczewska,

Dobosz

2024

Applied Sciences

View full text Add to dashboard Cite

Magnetite-based nanoparticles are of constant interest in the scientific community as potential systems for biomedical applications. Over the years, the ability to synthesize diverse systems based on iron (II, III) oxide nanoparticles has been mastered to maximize their potential effectiveness in the targeted delivery of active substances in cancer therapy. The present review explores recent literature findings that detail various magnetic nanosystems. These encompass straightforward designs featuring a polymer coating on the magnetic core and more intricate matrices for delivering chemotherapeutic drugs. This paper emphasizes novel synthetic approaches that impact the efficacy and progress of anticancer investigations, specifically targeting a particular cancer type. The research also delves into combinations with alternative treatment methods and diagnostic approaches. Additionally, it highlights a critical aspect—the interaction with cells—identifying it as the least developed aspect in current research on these systems.

show abstract

Section: Treatment Of Other Cancersmentioning

confidence: 99%

Section: Monitoring Of Magnetite-based Nanocarriers Interacting With ...mentioning

confidence: 99%

Recent Progress and Challenges Regarding Magnetite-Based Nanoparticles for Targeted Drug Delivery

Kurczewska,

Dobosz

2024

Applied Sciences

View full text Add to dashboard Cite

show abstract

“…al. conjugated folic acid with polyethylene glycol in a synthetic pathway involving six steps, which allowed a hybrid magnetic nanocarrier loaded with TMX that showed significant growth inhibition of MCF-7 breast cancer cells (Majd et al, 2013) (Khalil et al, 2022).…”

Section: Introductionmentioning

confidence: 99%

Magnetic hybrid nanomaterial based on a natural polymer and an amino acid as pH/temperature dual-responsive nanoplatform for the delivery of tamoxifen

Torres,

Cadena Castro,

Ancarani

et al. 2024

Front. Nanotechnol.

View full text Add to dashboard Cite

Magnetic hybrid nanomaterials offer promising properties for the advancement of nanoplatforms in cancer nanomedicine, particularly in drug delivery applications. These nanoplatforms can effectively respond to various stimuli present at the tumor site, such as pH and temperature fluctuations, allowing for controlled and triggered release of therapeutic payloads. In this study, we present a straightforward methodology for the synthesis of stable hybrid magnetic nanoplatforms (HMNP) based on Fe3O4 nanoparticles, L-cysteine (L-Cys), and hyaluronic acid (HA) as key constituents for the delivery of tamoxifen (TMX). The synthesized superparamagnetic HMNP, Fe3O4-L-Cys-HA, with a size of 11 nm, was successfully loaded with TMX. The incorporation of L-Cys showed superior interaction with the surface of Fe3O4 nanoparticles compared to other L-Cys derivatives explored as ligands. Consequently, L-Cys was selected for further functionalization with HA, providing the HMNP with active targeting properties toward CD44-overexpressed receptors. High loading efficiency of TMX (75%) was achieved via electrostatic interaction between the carboxylate groups exposed by the HMNP and the ammonium group of the TMX side chain. Efficient control in the TMX release towards different receptor media was observed. Notably, the release of TMX from HMNP-TMX was triggered under acidic pH and hyperthermia conditions, showcasing its responsiveness to both stimuli. Furthermore, enhanced anticancer activity of TMX against MDA-MB-231 breast cancer cells was observed when loaded into HMNP (IC50 almost 3-fold lower for HMNP-TMX compared to free TMX), indicating improved cell uptake of TMX-loaded HMNP in comparison to the free drug. Overall, pH/temperature dual-sensitive HMNP demonstrates promising potential as a nanoplatform for cancer nanomedicine, with prospects for magnetic hyperthermia therapy.

show abstract

“…Versatile NPs may improve controlled release for a desired time and maintain drug stability. Ligated NPs may be a promising system for precise drug delivery. − …”

Section: Introductionmentioning

confidence: 99%

Surface-Modified Biobased Polymeric Nanoparticles for Dual Delivery of Doxorubicin and Gefitinib in Glioma Cell Lines

et al. 2023

View full text Add to dashboard Cite

Glioma is a malignant form of brain cancer that is challenging to treat due to the progressive growth of glial cells. To target overexpressed folate receptors in glioma brain tumors, we designed and investigated doxorubicin–gefitinib nanoparticles (Dox-Gefit NPs) and folate conjugated Dox-Gefit NPs (Dox-Gefit NPs-F). Dox-Gefit NPs and Dox-Gefit NPs-F were characterized by multiple techniques including Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), differential scanning calorimetry (DSC), proton nuclear magnetic resonance (1H NMR), and transmission electron microscopy (TEM). In vitro release profiles were measured at both physiological and tumor endosomal pH. The cytotoxicity of the Dox-Gefit NP formulations was measured against C6 and U87 glioma cell lines. A hemolysis assay was performed to investigate biocompatibility of the formulations, and distribution of the drugs in different organs was also estimated. The Dox-Gefit NPs and Dox-Gefit NPs-F were 109.45 ± 7.26 and 120.35 ± 3.65 nm in size and had surface charges of −18.0 ± 3.27 and −20.0 ± 8.23 mV, respectively. Dox-Gefit NPs and Dox-Gefit NPs-F significantly reduced the growth of U87 cells, with IC50 values of 9.9 and 3.2 μM. Similarly, growth of the C6 cell line was significantly reduced, with IC50 values of 8.43 and 3.31 μM after a 24 h incubation, in Dox-Gefit NPs and Dox-Gefit NPs-F, respectively. The percentage drug releases of Dox and Gefit from Dox-Gefit NPs at pH 7.4 were 60.87 ± 0.59 and 68.23 ± 0.1%, respectively. Similarly, at pH 5.4, Dox and Gefit releases from NPs were 70.87 ± 0.28 and 69.24 ± 0.12%, respectively. Biodistribution analysis revealed that more Dox and Gefit were present in the brain than in the other organs. The functionalized NPs inhibited the growth of glioma cells due to high drug concentrations in the brain. Folate conjugated NPs of Dox-Gefit could be a treatment option in glioma therapy.

show abstract

Bifunctional folic-conjugated aspartic-modified Fe₃O₄ nanocarriers for efficient targeted anticancer drug delivery

Abstract: Aspartic acid was used as an anchoring molecule for the conjugation of folic acid and doxorubicin to Fe3O4 nanoparticles. The as-prepared bifunctional folic-conjugated aspartic-modified Fe3O4 nanocarrier was shown to be as an efficient targeted anticancer drug delivery.

Cited by 9 publications

References 62 publications

Recent Progress and Challenges Regarding Magnetite-Based Nanoparticles for Targeted Drug Delivery

Recent Progress and Challenges Regarding Magnetite-Based Nanoparticles for Targeted Drug Delivery

Magnetic hybrid nanomaterial based on a natural polymer and an amino acid as pH/temperature dual-responsive nanoplatform for the delivery of tamoxifen

Surface-Modified Biobased Polymeric Nanoparticles for Dual Delivery of Doxorubicin and Gefitinib in Glioma Cell Lines

Contact Info

Product

Resources

About

Bifunctional folic-conjugated aspartic-modified Fe3O4 nanocarriers for efficient targeted anticancer drug delivery

Abstract: Aspartic acid was used as an anchoring molecule for the conjugation of folic acid and doxorubicin to Fe3O4 nanoparticles. The as-prepared bifunctional folic-conjugated aspartic-modified Fe3O4 nanocarrier was shown to be as an efficient targeted anticancer drug delivery.

Cited by 9 publications

References 62 publications

Recent Progress and Challenges Regarding Magnetite-Based Nanoparticles for Targeted Drug Delivery

Recent Progress and Challenges Regarding Magnetite-Based Nanoparticles for Targeted Drug Delivery

Magnetic hybrid nanomaterial based on a natural polymer and an amino acid as pH/temperature dual-responsive nanoplatform for the delivery of tamoxifen

Surface-Modified Biobased Polymeric Nanoparticles for Dual Delivery of Doxorubicin and Gefitinib in Glioma Cell Lines

Contact Info

Product

Resources

About

Bifunctional folic-conjugated aspartic-modified Fe₃O₄ nanocarriers for efficient targeted anticancer drug delivery