Bifunctional Fluorescent Probe for Sequential Sensing of Thiols and Primary Aliphatic Amines in Distinct Fluorescence Channels

Abstract

Thiols and primary aliphatic amines (PAA) are ubiquitous and extremely important species in biological systems. They perform significant interplaying roles in complex biological events. A single fluorescent probe differentiating both thiols and PAA can contribute to understanding the intrinsic inter-relationship of thiols and PAA in biological processes. Herein, we rationally constructed the first fluorescent probe that can respond to thiols and PAA in different fluorescence channels. The probe exhibited a high selectivity and sensitivity to thiols and PAA. In addition, it displayed sequential sensing ability when the thiols and PAA coexisted. The application experiments indicated that the probe can be used for sensing thiols and PAA in human blood serum. Moreover, the fluorescence imaging of endogenous thiols and PAA as well as antihypertensive drugs captopril and amlodipine in living cells were successfully conducted.

Surface Plasmon Coupled Directional Emission for Integrated Fluorescence‐Raman Biodetection: A Proof‐of‐Concept Study

“…It is considered as a powerful detection method in biochemical analysis, cell imaging, drug release and cancer diagnosis. [1][2][3][4] Noble metal nanostructures produced strong near field enhancements which support enhanced fluorescence and Raman spectra. [5,6] On the other hand, metal nanostructures as well increase Raman signals of molecules nearby, namely surface enhanced Raman scattering (SERS) with advantages of fingerprint recognition characteristics, good spectrum stability, and narrow spectral line bandwidth.…”

“…Fluorescence analysis has many advantages such as high sensitivity, good selectivity, non‐invasiveness and wide applicability. It is considered as a powerful detection method in biochemical analysis, cell imaging, drug release and cancer diagnosis [1–4] . Noble metal nanostructures produced strong near field enhancements which support enhanced fluorescence and Raman spectra [5,6] .…”