Bifunctional cationic solid lipid nanoparticles of β-NaYF<sub>4</sub>:Yb,Er upconversion nanoparticles coated with a lipid for bioimaging and gene delivery

Song, Chun; Zhang, Shubiao; Zhou, Quan; Shi, Lei; Du, Linying; Zhi, Defu; Zhao, Yinan; Zhen, Yuhong; Zhao, Defeng

doi:10.1039/c7ra02683h

Cited by 14 publications

(6 citation statements)

References 43 publications

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“…In some cases, additional characterization techniques have been used to identify lipids in NP lipid coatings. Fluorescence microscopy, X-ray photoelectron spectroscopy, and Fourier transform infrared spectroscopy have been used to provide information about the types of lipids on a NP surface but cannot provide quantitative compositional information. Inductively coupled plasma mass spectrometry (ICP-MS) can be used to quantify phospholipids using total phosphorus content, and thermogravimetric analysis (TGA) can quantify the total amount of lipids on a NP; however, neither can distinguish the relative ratio of different lipid components.…”

Section: Introductionmentioning

confidence: 99%

Scratching the Surface (Modification): Developing a Quantitative Liquid Chromatography–Tandem Mass Spectrometry Method for the Investigation of PEGylated and Non-PEGylated Lipid Mixtures on Lipid-Coated Lanthanide Nanoparticles

et al. 2021

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We are interested in developing lanthanide nanoparticles (LnNPs) of the general formula NaLnF 4 as high-sensitivity reagents for mass cytometry. These LnNPs must be coated to provide colloidal stability in aqueous buffer and functionality for detecting cellular biomarkers. Lipid bilayer coatings are a promising approach, but one requires an analytical technique to enable characterization of the NP coating composition as opposed to the composition of the lipid formulation used in the coating process. However, quantification of the lipid composition of lipid coatings on polymer and inorganic NPs is not common practice in the field.Here we describe a method based on high-performance liquid chromatography (LC) for separations and triple quadrupole tandem mass spectrometry (MS/MS) instrumentation for detection and show that it can quantify complex lipid mixtures using small (<1 μg) amounts of sample. Our lipid formulation consisted of a mixture of egg sphingomyelin, 1,2-dioleoyl-sn-glycero-3-phosphocholine, 1,2-dioleoyl-3-trimethylammonium-propane, cholesterol-PEG600, and 1,2-distearoyl-sn-glycero-3-phosphoethanolamine-N-[biotinyl(polyethylene glycol)-2000]. We were able to extract the coating from lipid-coated 35 nm diameter LnNPs, quantify the lipid/NP ratio, and show that the coating composition differed from the composition of the lipid formulation for several of the species. Knowledge of the actual composition of the lipid coating for lipidcoated NPs is critical for designing and optimizing application of these materials. Our results establish the value of LC-MS/MS characterization of lipid-coated NPs, thus providing an important new addition to the toolbox available for characterizing these types of nanomaterials.

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Section: Introductionmentioning

confidence: 99%

Scratching the Surface (Modification): Developing a Quantitative Liquid Chromatography–Tandem Mass Spectrometry Method for the Investigation of PEGylated and Non-PEGylated Lipid Mixtures on Lipid-Coated Lanthanide Nanoparticles

et al. 2021

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“…Much progress has been made toward achieving structural diversity of the NaYF 4 :Yb,Er-based nanomaterials for a variety of applications. 33 Notable examples are metal–organic framework/NaYF 4 :Ln core–shells for NIR-enhanced photocatalysis, 34 NaYF 4 :Yb,Er nanoparticles for latent fingerprints, 35 NaYF 4 :Yb,Er UC/magnetite/dye nanocomposites for oxygen sensing, 36 lipid-coated NaYF 4 :Yb,Er nanoparticles for bioimaging and gene delivery, 37 and CdSe/NaYF 4 :Yb,Er nanohybrids for photovoltaics. 38 Owing to the inherent characteristics of low-energy light absorption with minimal cell damage, the NaYF 4 :Yb,Er nanoparticles are extensively used in biomedicine.…”

Section: Resultsmentioning

confidence: 99%

Biocompatible Chitosan-Functionalized Upconverting Nanocomposites

et al. 2018

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Simultaneous integration of photon emission and biocompatibility into nanoparticles is an interesting strategy to develop applications of advanced optical materials. In this work, we present the synthesis of biocompatible optical nanocomposites from the combination of near-infrared luminescent lanthanide nanoparticles and water-soluble chitosan. NaYF4:Yb,Er upconverting nanocrystal guests and water-soluble chitosan hosts are prepared and integrated together into biofunctional optical composites. The control of aqueous dissolution, gelation, assembly, and drying of NaYF4:Yb,Er nanocolloids and chitosan liquids allowed us to design novel optical structures of spongelike aerogels and beadlike microspheres. Well-defined shape and near-infrared response lead upconverting nanocrystals to serve as photon converters to couple with plasmonic gold (Au) nanoparticles. Biocompatible chitosan-stabilized Au/NaYF4:Yb,Er nanocomposites are prepared to show their potential use in biomedicine as we find them exhibiting a half-maximal effective concentration (EC50) of 0.58 mg mL–1 for chitosan-stabilized Au/NaYF4:Yb,Er nanorods versus 0.24 mg mL–1 for chitosan-stabilized NaYF4:Yb,Er after 24 h. As a result of their low cytotoxicity and upconverting response, these novel materials hold promise to be interesting for biomedicine, analytical sensing, and other applications.

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“…Among these options, lipids and lipid-like materials have demonstrated superior efficacy as delivery agents due to their exceptional stability within the circulatory system, high fusogenicity with cell membranes, and minimal cytotoxicity. [15][16][17] The concept of artificial lipid vesicles, or liposomes, was pioneered by hematologist Alec Bangham. [18] Since their inception, liposomes have gained widespread recognition and adoption as versatile delivery vehicles for pharmaceutical compounds.…”

Section: Introductionmentioning

confidence: 99%

Synthesis of Amino Acid‐Based Cationic Lipids and Study of the Role of the Cationic Head Group for Enhanced Drug and Nucleic Acid Delivery

Mandal,

Mallik,

Bhoumick

et al. 2024

ChemBioChem

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Leveraging liposomes for drug and nucleic acid delivery, though promising due to reduced toxicity and ease of preparation, faces challenges in stability and efficiency. To address this, we synthesized cationic amphiphiles from amino acids (arginine, lysine, and histidine). Histidine emerged as the superior candidate, leading to the development of three histidine‐rich cationic amphiphiles for liposomes. Using the hydration method, we have prepared the liposomes and determined the optimal N/P ratios for lipoplex formation via gel electrophoresis. In vitro transfection assays compared the efficacy of our lipids to Fugene, while MTT assays gauged biocompatibility across cancer cell lines (MDA‐MB 231 and MCF‐7). The histidine‐based lipid demonstrated marked potential in enhancing drug and nucleic acid delivery. This improvement stemmed from increased zeta potential, enhancing electrostatic interactions with nucleic acids and cellular uptake. Our findings underscore histidine's crucial role over lysine and arginine for effective delivery, revealing a significant correlation between histidine abundance and optimal performance. This study paves the way for histidine‐enriched lipids as promising candidates for efficient drug and nucleic acid delivery, addressing key challenges in the field.

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Bifunctional cationic solid lipid nanoparticles of β-NaYF₄:Yb,Er upconversion nanoparticles coated with a lipid for bioimaging and gene delivery

Abstract: We demonstrate the possibility of novel bifunctional cationic solid lipid nanoparticles (CSLNs) for bioimaging and gene delivery through peptide lipid coated UCNPs.

Cited by 14 publications

References 43 publications

Scratching the Surface (Modification): Developing a Quantitative Liquid Chromatography–Tandem Mass Spectrometry Method for the Investigation of PEGylated and Non-PEGylated Lipid Mixtures on Lipid-Coated Lanthanide Nanoparticles

Scratching the Surface (Modification): Developing a Quantitative Liquid Chromatography–Tandem Mass Spectrometry Method for the Investigation of PEGylated and Non-PEGylated Lipid Mixtures on Lipid-Coated Lanthanide Nanoparticles

Biocompatible Chitosan-Functionalized Upconverting Nanocomposites

Synthesis of Amino Acid‐Based Cationic Lipids and Study of the Role of the Cationic Head Group for Enhanced Drug and Nucleic Acid Delivery

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Bifunctional cationic solid lipid nanoparticles of β-NaYF4:Yb,Er upconversion nanoparticles coated with a lipid for bioimaging and gene delivery

Abstract: We demonstrate the possibility of novel bifunctional cationic solid lipid nanoparticles (CSLNs) for bioimaging and gene delivery through peptide lipid coated UCNPs.

Cited by 14 publications

References 43 publications

Scratching the Surface (Modification): Developing a Quantitative Liquid Chromatography–Tandem Mass Spectrometry Method for the Investigation of PEGylated and Non-PEGylated Lipid Mixtures on Lipid-Coated Lanthanide Nanoparticles

Scratching the Surface (Modification): Developing a Quantitative Liquid Chromatography–Tandem Mass Spectrometry Method for the Investigation of PEGylated and Non-PEGylated Lipid Mixtures on Lipid-Coated Lanthanide Nanoparticles

Biocompatible Chitosan-Functionalized Upconverting Nanocomposites

Synthesis of Amino Acid‐Based Cationic Lipids and Study of the Role of the Cationic Head Group for Enhanced Drug and Nucleic Acid Delivery

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Bifunctional cationic solid lipid nanoparticles of β-NaYF₄:Yb,Er upconversion nanoparticles coated with a lipid for bioimaging and gene delivery