Bifunctional aptamer-mediated catalytic hairpin assembly for the sensitive and homogenous detection of rare cancer cells

Liu, Jumei; Zhang, Ye; Zhao, Qianwen; Situ, Bo; Zhao, Jiamin; Luo, Shihua; Li, Bo; Yan, Xiaohui; Vadgama, Pankaj; Su, Lei; Ma, Wen; Wen, Wei; Zheng, Lei

doi:10.1016/j.aca.2018.04.068

Cited by 29 publications

(15 citation statements)

References 44 publications

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“…Various CHA systems and analytical platforms can be applied. CHA based aptasensors for cancer cells exhibit high sensitivity down to 10 cell mL −1 and satisfactory selectivity between the target and interferent cells . These cytosensors rely heavily on the development of aptamers.…”

Section: Cha Based Biosensors For Various Targetsmentioning

confidence: 99%

“…Cancer cell biosensors based on CHA. A) CHA‐based cytosensor with aptamer . B) CHA‐based cytosensor with Ab …”

Section: Cha Based Biosensors For Various Targetsmentioning

confidence: 99%

“…Although the entity of CHA is nucleic acid strand displacement, the detection targets are not confined to nucleic acids. Many other analytes including metal ions, proteins, enzymes, and even cancer cells have been measured based on CHA.…”

Section: Introductionmentioning

confidence: 99%

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Applications of Catalytic Hairpin Assembly Reaction in Biosensing

Liu

Zhang

Xie

et al. 2019

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Nucleic acids are considered as perfect programmable materials for cascade signal amplification and not merely as genetic information carriers. Among them, catalytic hairpin assembly (CHA), an enzyme‐free, high‐efficiency, and isothermal amplification method, is a typical example. A typical CHA reaction is initiated by single‐stranded analytes, and substrate hairpins are successively opened, resulting in thermodynamically stable duplexes. CHA circuits, which were first proposed in 2008, present dozens of systems today. Through in‐depth research on mechanisms, the CHA circuits have been continuously enriched with diverse reaction systems and improved analytical performance. After a short time, the CHA reaction can realize exponential amplification under isothermal conditions. Under certain conditions, the CHA reaction can even achieve 600 000‐fold signal amplification. Owing to its promising versatility, CHA is able to be applied for analysis of various markers in vitro and in living cells. Also, CHA is integrated with nanomaterials and other molecular biotechnologies to produce diverse readouts. Herein, the varied CHA mechanisms, hairpin designs, and reaction conditions are introduced in detail. Additionally, biosensors based on CHA are presented. Finally, challenges and the outlook of CHA development are considered.

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Section: Cha Based Biosensors For Various Targetsmentioning

confidence: 99%

“…Cancer cell biosensors based on CHA. A) CHA‐based cytosensor with aptamer . B) CHA‐based cytosensor with Ab …”

Section: Cha Based Biosensors For Various Targetsmentioning

confidence: 99%

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Applications of Catalytic Hairpin Assembly Reaction in Biosensing

Liu

Zhang

Xie

et al. 2019

Small

Self Cite

213

124

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“…This SiNW-based microfluidic chip platform can not only improve CTC capture efficiency, but can also realize controllable CTC release via nuclease treatment. In another report, Liu et al developed a homogeneous fluorescent method to detect cancer cells based on catalytic hairpin assembly (CHA) and bifunctional aptamers ( Figure 3 D) [ 129 ]. The strategy showed high specificity for discriminating normal cells and leukocytes, and the detection limit was 10 cells/mL.…”

Section: Development Of Cancer-specific Aptamers For Diagnosis and Treatmentmentioning

confidence: 99%

“…They are highly sensitive, easy to manipulate, and can be conveniently combined with various different isolation methods. However, autofluorescence background is a major issue, interfering with the sensitive and reliable detection of CTCs [ 129 , 141 , 142 , 143 , 144 ]. To overcome this disadvantage, Liu’s group labeled tumor cells with aptamer-conjugated upconversion nanoparticles (UCNPs).…”

Section: Development Of Cancer-specific Aptamers For Diagnosis and Treatmentmentioning

confidence: 99%

The Potential of Aptamer-Mediated Liquid Biopsy for Early Detection of Cancer

Roy¹,

Pascher

Juratli

et al. 2021

IJMS

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The early detection of cancer favors a greater chance of curative treatment and long-term survival. Exciting new technologies have been developed that can help to catch the disease early. Liquid biopsy is a promising non-invasive tool to detect cancer, even at an early stage, as well as to continuously monitor disease progression and treatment efficacy. Various methods have been implemented to isolate and purify bio-analytes in liquid biopsy specimens. Aptamers are short oligonucleotides consisting of either DNA or RNA that are capable of binding to target molecules with high specificity. Due to their unique properties, they are considered promising recognition ligands for the early detection of cancer by liquid biopsy. A variety of circulating targets have been isolated with high affinity and specificity by facile modification and affinity regulation of the aptamers. In this review, we discuss recent progress in aptamer-mediated liquid biopsy for cancer detection, its associated challenges, and its future potential for clinical applications.

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