Bifidobacterium moukalabense sp. nov., isolated from the faeces of wild west lowland gorilla (Gorilla gorilla gorilla)

Abstract

Gram-staining-positive anaerobic rods were isolated from the faeces of a wild lowland gorilla (Gorilla gorilla gorilla) in Moukalaba-Doudou National Park, Gabon, and strain GG01 T was taxonomically investigated. Based on phylogenetic analyses and specific phenotypic characteristics, the strain belonged to the genus Bifidobacterium. Phylogenetic analysis of its 16S rRNA gene sequence revealed that strain GG01 T formed a single monophyletic cluster and had a distinct line of descent. Based on 16S rRNA gene sequence similarity, the type strains of Bifidobacterium catenulatum JCM 1194 T (98.3 %) and Bifidobacterium pseudocatenulatum (98.1 %) JCM 1200 T were the most closely related to this novel strain, although it was clear that they belonged to different species. hsp60 sequences also supported these relationships. The DNA G+C content of this novel strain was 60.1 mol%. Bifidobacterium moukalabense sp. nov. (type strain GG01 T 5JCM 18751 T 5DSM 27321 T ) is proposed.

Isolation and identification of cultivable Bifidobacterium spp. from the faeces of 5 baby common marmosets (Callithrix jacchus L.)

“…The marked presence in baby marmosets of B. aesculapii, never found in adult marmoset, could suggest a predilection of the species for the gut habitat of baby individuals, a situation similar to humans where some bifidobacterial species are peculiar only to infants [1]. Unlike other studies concerning bifidobacteria in non-human primates, no bifidobacterial human species have been found in baby common marmosets [2,3,6]. …”

“…In recent years there has been important progress in gut microbiota studies based on culture independent techniques, however only culture dependent techniques can be used when the aim is to identify and characterize isolates. Unlike studies of human gut microbiota, those addressing the bifidobacteria of non-human primates are very few: Bifidobacterium angulatum and Bifidobacterium moukalabense, isolated from wild chimpanzee [2,3]; Bifidobacterium dentium and Bifidobacterium adolescentis from chimpanzees and orangutan [4]; Bifidobacterium aesculapii, Bifidobacterium callithricos and Bifidobacterium reuteri from common marmoset (Callithrix jacchus) [5,6] and Bifidobacterium saguini, Bifidobacterium biavatii and Bifidobacterium stellenboschense from red-handed tamarind [6]. The aim of the present work was to characterize the bifidobacterial cultivable microbiota of common marmoset babies, a completely unknown habitat.…”

Bifidobacterium aerophilum sp. nov., Bifidobacterium avesanii sp. nov. and Bifidobacterium ramosum sp. nov.: Three novel taxa from the faeces of cotton-top tamarin ( Saguinus oedipus L.)

“…Bifidobacterium from various animals, including humans, in order to understand how they are mostly 79 distributed [5,39] and, especially, which are their phenotypic and genotypic characteristics, thus allowing the 80 reconstruction of a more robust bifidobacterial phylogeny. 81…”

Bifidobacterium eulemuris sp. nov., isolated from faeces of black lemurs (Eulemur macaco)

“…As a result, 11 novel bifidobacterial species have been described from the prosimian Lemur catta and from two New World monkey species, Callithrix jacchus and Saguinus midas (Endo et al, 2012;Michelini et al, 2015Michelini et al, , 2016Modesto et al, 2014). From apes, the novel species Bifidobacterium moukalabense was isolated from the faeces of a wild gorilla (Gorilla gorilla gorilla) (Tsuchida et al, 2014). Furthermore, Bifidobacterium angulatum was isolated from an adult female chimpanzee (Pan troglodytes verus) (Tsuchida et al, 2014;Ushida et al, 2010) and Bifidobacterium dentium and Bifidobacterium adolescentis were isolated from chimpanzee (Pan troglodytes verus) and orangutan (Pongo pygmaeus), respectively (D'Aimmo et al, 2012); these three latter species are usually found in humans.…”