Bifidobacterium longum subsp. infantis CECT 7210 Reduces Inflammatory Cytokine Secretion in Caco-2 Cells Cultured in the Presence of Escherichia coli CECT 515

Abstract

Previous works have described the activity of Bifidobacterium longum subsp. infantis CECT 7210 (also commercially named B. infantis IM-1®) against rotavirus in mice and intestinal pathogens in piglets, as well as its diarrhea-reducing effect on healthy term infants. In the present work, we focused on the intestinal immunomodulatory effects of B. infantis IM-1® and for this purpose we used the epithelial cell line isolated from colorectal adenocarcinoma Caco-2 and a co-culture system of human dendritic cells (DCs) from peripheral blood together with Caco-2 cells. Single Caco-2 cultures and Caco-2: DC co-cultures were incubated with B. infantis IM-1® or its supernatant either in the presence or absence of Escherichia coli CECT 515. The B. infantis IM-1® supernatant exerted a protective effect against the cytotoxicity caused by Escherichia coli CECT 515 on single cultures of Caco-2 cells as viability reached the values of untreated cells. B. infantis IM-1® and its supernatant also decreased the secretion of pro-inflammatory cytokines by Caco-2 cells and the co-cultures incubated in the presence of E. coli CECT 515, with the response being more modest in the latter, which suggests that DCs modulate the activity of Caco-2 cells. Overall, the results obtained point to the immunomodulatory activity of this probiotic strain, which might underlie its previously reported beneficial effects.

The Gliadin Hydrolysis Capacity of B. longum, L. acidophilus, and L. plantarum and Their Protective Effects on Caco-2 Cells against Gliadin-Induced Inflammatory Responses

“…As Bifidobacteria and Lactobacilli strains are known to be the most common probiotics used in foods and supplements, we evaluated the effect of a probiotic mix containing B. longum, L. acidophilous, and L. plantarum on degrading gliadin peptides of wheat flour dough and reducing their inflammatory effects in a Caco-2 cell line. Similar to previous in vitro studies, we observed the inflammatory effect of peptic-tryptic digested gliadin peptides (extracted from non-fermented dough) on the Caco-2 intestinal cells [58,59]. Our results also showed the capacity of B. longum, L. acidophilous, and L. plantarum to reduce pro-inflammatory and increase anti-inflammatory cytokine expression by these cells.…”

“…Álvarez-Mercado et al, in a recent study, observed how B. longum subsp. Infantis reduced the secretion of pro-inflammatory cytokines by Caco-2 cells [ 59 ]. Another study, conducted by Zhao et al, reported that B. longum subsp.…”

Alterations in the intestinal microbiome and metabolic profile of patients with cirrhosis supplemented with lactulose, Clostridium butyricum, and Bifidobacterium longum infantis: a randomized placebo-controlled trial

“…Additionally, Clostridium butyricum combined with rosuvastatin ameliorates imbalances in the intestinal microbiota, liver fibrosis, and liver functional defects in patients with NAFLD ( Zhu et al, 2022 ). Studies have also shown that Bifidobacterium longum infantis has health-promoting effects, such as reductions of pro-inflammatory cytokine concentration in intestinal epithelial cells ( Ehrlich et al, 2020 ; Wang et al, 2022 ) and Caco-2 cells ( Alvarez-Mercado et al, 2022 ). One study effectively applied lactulose with or without rifaximin to prevent hepatic encephalopathy ( Rahimi et al, 2021 ), which was verified by a multi-center randomized, double blind, placebo-controlled trial ( de Wit et al, 2020 ).…”

Bifidobacterium species viability in dairy-based probiotic foods: challenges and innovative approaches for accurate viability determination and monitoring of probiotic functionality

“…A significant quantity of in vitro and in vivo evidence has demonstrated the probiotic functionalities of Bifidobacterium spp. (Konieczna I. et al, 2012;Groeger et al, 2013;Turroni et al, 2014;Din et al, 2020;Shang et al, 2020;Zhang et al, 2020;van der Hee and Wells, 2021;Schiweck et al, 2022;Álvarez-Mercado et al, 2022;He et al, 2023). These diverse probiotic functionalities include the enhancement of the host immune system, protection against communicable and non-communicable diseases, as well as improvement of nutritional metabolism (He et al, 2023).…”