Bifidobacterium adolescentis Exerts Strain-Specific Effects on Constipation Induced by Loperamide in BALB/c Mice

Abstract

Constipation is one of the most common gastrointestinal complaints worldwide. This study was performed to determine whether Bifidobacterium adolescentis exerts inter-strain differences in alleviating constipation induced by loperamide in BALB/c mice and to analyze the main reasons for these differences. BALB/c mice underwent gavage with B. adolescentis (CCFM 626, 667, and 669) once per day for 17 days. The primary outcome measures included related constipation indicators, and the secondary outcome measures were the basic biological characteristics of the strains, the concentration changes of short-chain fatty acids in feces, and the changes in the fecal flora. B. adolescentis CCFM 669 and 667 relieved constipation symptoms by adhering to intestinal epithelial cells, growing quickly in vitro and increasing the concentrations of propionic and butyric acids. The effect of B. adolescentis on the gut microbiota in mice with constipation was investigated via 16S rRNA metagenomic analysis. The results revealed that the relative abundance of Lactobacillus increased and the amount of Clostridium decreased in the B. adolescentis CCFM 669 and 667 treatment groups. In conclusion, B. adolescentis exhibits strain-specific effects in the alleviation of constipation, mostly due to the strains’ growth rates, adhesive capacity and effects on the gut microbiome and microenvironment.

Preventive Effect of Lactobacillus Plantarum CQPC10 on Activated Carbon Induced Constipation in Institute of Cancer Research (ICR) Mice

“…MTL has been used to evaluate gastrointestinal tract peristalsis and it has been widely considered to promote the mobility of the gastrointestinal tract, while decreased release will slow peristalsis [22]. Gas is an important gastrointestinal hormone, which has been shown to promote gastric secretion, improve peristalsis, accelerate gastric emptying, and promote pyloric sphincter relaxation [23]. Currently, AchE has been considered as one of two neurotransmitters that play a very important role in the motility of the intestinal tract.…”

Comparative Study of Constipation Exacerbation by Potassium Binders Using a Loperamide-Induced Constipation Model

“…The total number of fecal pellets is shown in Figure 1a; the cumulative number of fecal pellets over time is shown in Figure 1b. The number of fecal pellets is one of the parameters for evaluating constipation, which reduces with constipation [19]. The total number of fecal pellets was significantly reduced in the Lop group compared to that in the control group and similarly decreased in the Lop + CPS group (Control: 46.4 ± 10.7 pellets/18 h, Lop: 29.9 ± 7.7 pellets/18 h, Lop + CPS: 29.6 ± 7.1 pellets/18 h).…”

“…Gastrointestinal transit time (GTT) is shown in Figure 4. GTT is the time between ingestion of food and defecation and is considered one of the parameters to evaluate constipation, as it is longer with constipation [19]. GTT was significantly longer in the Lop group compared to that in the control group (Control: 4.4 ± 0.8 h, Lop: 6.1 ± 0.4 h).…”

Inhibitory Effect of Lactobacillus plantarum CQPC02 Isolated from Chinese Sichuan Pickles (Paocai) on Constipation in Mice

“…MTL can stimulate pepsin production and promote intestinal motility, and a reduction in MTL release will reduce gastrointestinal peristalsis [24]. Gas has a significant role in alleviating constipation because it can promote gastrointestinal secretion and gastrointestinal motility, as well as promoting pyloric relaxation [25]. AChE is an important neurotransmitter in intestinal motility, and it can regulate muscle contraction and mucus secretion, relax muscles, and promote the excretion of feces [26].…”