Bifidobacteria in the digestive tract of bumblebees

Killer, Jiří; Kopečný, J.; Mrázek, Jakub; Rada, V.; Dubná, Soňa; Marounek, M.

doi:10.1016/j.anaerobe.2009.07.007

Cited by 32 publications

(24 citation statements)

References 41 publications

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“…Optimum temperature for growth is 37 u C, with a minimum of 10 u C and a maximum of 40 u C. No growth occurs at 5 or 45 u C. In TPY broth, the minimum initial pH for growth is pH 5 within a period of 24-48 h (weak growth observed at pH 4.5, and no growth observed at . Data are from this study and previous studies (Scardovi, 1986;Scardovi & Trovatelli, 1969;Killer et al, 2009Killer et al, , 2010. All insect strains ferment ribose, salicin, glucose, gentiobiose and arbutin.…”

Section: Description Of Bifidobacterium Actinocoloniiforme Sp Novmentioning

confidence: 99%

“…The GenBank/EMBL/DDBJ accession numbers for the 16S rRNA gene sequences of strains LISLUCIII-P2 (Killer et al, 2010), which was found to be distinct from the bifidobacterial populations in honeybees. The first named representative of the bumblebee bifidobacteria, Bifidobacterium bombi, has been described recently (Killer et al, 2009).…”

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confidence: 99%

“…Here, we report the results of a taxonomic study of two further representatives of the bumblebee bifidobacteria. Bacterial strains were collected from the digestive tracts of three species of bumblebee, as described previously (Killer et al, 2010). Isolates LISLUCIII-P2 T , LUCL-P3, JEMLUCVIII-4 T and JEMLUCVII-1 were taken as representatives of the two newly described species.…”

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confidence: 99%

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Bifidobacterium actinocoloniiforme sp. nov. and Bifidobacterium bohemicum sp. nov., from the bumblebee digestive tract

Killer

Kopečný

Mrázek

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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Our previous study, based primarily on PCR-denaturing gradient gel electrophoresis and 16S rRNA gene sequencing, focused on the isolation of four bifidobacterial groups from the digestive tract of three bumblebee species. In that study, we proposed that these isolated groups potentially represented novel species of the family Bifidobacteriaceae. One of the four, Bifidobacterium bombi, has been described recently. Strains representing two of the other groups have been classified as members of the genus Bifidobacterium on the basis of positive results for fructose-6-phosphate phosphoketolase activity and analysis of partial 16S rRNA and heat-shock protein 60 (hsp60) gene sequences. Analysis of 16S rRNA gene sequence similarities revealed that the isolates of the first group were affiliated to Bifidobacterium asteroides YIT 11866T, B. indicum JCM 1302T and B. coryneforme ATCC 25911T (96.2, 96.0 and 95.9 % sequence similarity, respectively), together with other bifidobacteria showing lower sequence similarity. Additional representatives of the second group were found to be affiliated to Bifidobacterium minimum YIT 4097T and B. coryneforme ATCC 25911T (96.0 and 96.3 % sequence similarity) and also to other bifidobacteria with lower sequence similarity. These results indicate that the isolates of the two groups belong to novel species within the genus Bifidobacterium. This observation was further substantiated by the results of partial sequencing of hsp60. On the basis of phylogenetic and phenotypic analyses and analysis of 16S rRNA and partial hsp60 gene sequences, we propose two novel species, Bifidobacterium actinocoloniiforme sp. nov. (type strain LISLUCIII-P2T = DSM 22766T = CCM 7728T) and Bifidobacterium bohemicum sp. nov. (type strain JEMLUCVIII-4T = DSM 22767T = CCM 7729T).

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Section: Description Of Bifidobacterium Actinocoloniiforme Sp Novmentioning

confidence: 99%

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confidence: 99%

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confidence: 99%

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Bifidobacterium actinocoloniiforme sp. nov. and Bifidobacterium bohemicum sp. nov., from the bumblebee digestive tract

Killer

Kopečný

Mrázek

et al. 2011

International Journal of Systematic and Evolutionary Microbiology

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“…Some researchers believe that the bacterial symbionts of insects, especially lactic acid bacteria, can influence the immune system and host health positively to some extent (Evans & Armstrong, 2006;Forsgren et al, 2010;Koch & SchmidHempel, 2012;Mattila et al, 2012;Vásquez et al, 2012). Many studies have been published in recent years on the prevalence of lactobacilli and other lactic acid bacteria in the digestive tracts of insects, especially in the digestive tracts of important pollinators (Mohr & Tebbe, 2006;Killer et al, 2010bKiller et al, , 2014aTajabadi et al, 2011;Martinson et al, 2012;Tang et al, 2012). Some authors have shown that novel, probably host-specific, species of lactobacilli are present in the stomachs of honeybees (Olofsson & Vásquez, 2008;Forsgren et al, 2010).…”

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confidence: 99%

“…Some authors have shown that novel, probably host-specific, species of lactobacilli are present in the stomachs of honeybees (Olofsson & Vásquez, 2008;Forsgren et al, 2010). The bacteria that inhabit the digestive tracts of bumblebees have not been adequately explored (Killer et al, 2010b;Koch & Schmid-Hempel, 2011). Studies on the isolation and characterization of bacteria present in the digestive tracts of bumblebees have so far focused only on representatives of the family Bifidobacteriaceae.…”

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Lactobacillus bombi sp. nov., from the digestive tract of laboratory-reared bumblebee queens (Bombus terrestris)

Killer

Votavová²,

Valterová

et al. 2014

International Journal of Systematic and Evolutionary Microbiology

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Lactobacillus bombi sp. nov., from the digestive tract of laboratory-reared bumblebee queens (Bombus terrestris) Three bacterial strains belonging to the genus Lactobacillus were isolated from the digestive tracts of laboratory-reared bumblebee queens (Bombus terrestris) using MRS agar under anaerobic conditions. The isolates were identified according to 16S rRNA gene sequence analysis as undescribed members of the genus Lactobacillus, with the highest 16S rRNA gene sequence similarity (96.9 %) to the uncharacterized bacterial strain Lactobacillus sp. Mboho2r2 isolated from the stomach of a European honeybee (Apis mellifera). Lactobacillus tucceti was found to be the closest related species with a validly published name, with 92.9 % 16S rRNA gene sequence similarity to the type strain. However, phylogenetic analyses based on different markers revealed that this species is phylogenetically very distant from the novel strains. The DNA G+C content of the proposed type strain BTLCH M1/2 T is 37.8 mol%. The fatty acids C 19 : 1 v6c and/or C 19 : 0 cyclo v10c/19v6, C 18 : 1 v9c and C 16 : 0 were predominant in all strains. Diphosphatidylglycerol, phosphatidylglycerol, a phospholipid, seven glycolipids and two phosphoglycolipids were detected in the novel strains. Growth was observed at 47 6C. The peptidoglycan type A4a L-Lys-D-Asp was determined for strain BTLCH M1/2 T. Genotypic characteristics and phylogenetic analyses based on the phylogenetic markers hsp60, pheS, rpoA and tuf as well as phenotypic characteristics and the results of chemotaxonomic analyses confirmed that the new isolates belong to a novel species of the genus Lactobacillus, for which the name Lactobacillus bombi sp. nov. is proposed. The type strain is BTLCH M1/2 T (5DSM 26517 T 5CCM 8440 T).

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Comparative genomics of Bifidobacterium species isolated from marmosets and humans

Brown

Mtui

Oswald

et al. 2019

American J Primatol

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The genus Bifidobacterium is purported to have beneficial consequences for human health and is a major component of many gastrointestinal probiotics. Although species of Bifidobacterium are generally at low relative frequency in the adult human gastrointestinal tract, they can constitute high proportions of the gastrointestinal communities of adult marmosets. To identify genes that might be important for the maintenance of Bifidobacterium in adult marmosets, ten strains of Bifidobacterium were isolated from the feces of seven adult marmosets, and their genomes were sequenced. There were six B. reuteri strains, two B. callitrichos strains, one B. myosotis sp. nov. and one B. tissieri sp. nov. among our isolates. Phylogenetic analysis showed that three of the four species we isolated were most closely related to B. bifidum, B. breve and B. longum, which are species found in high abundance in human infants. There were 1357 genes that were shared by at least one strain of B. reuteri, B. callitrichos, B. breve, and B. longum, and 987 genes that were found in all strains of the four species. There were 106 genes found in B. reuteri and B. callitrichos but not in human bifidobacteria, and several of these genes were involved in nutrient uptake. These pathways for nutrient uptake appeared to be specific to Bifidobacterium from New World monkeys. Additionally, the distribution of Bifidobacterium in fecal samples from captive adult marmosets constituted as much as 80% of the gut microbiome, although this was variable between individuals and colonies. We suggest that nutrient transporters may be important for the maintenance of Bifidobacterium during adulthood in marmosets.

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Bifidobacteria in the digestive tract of bumblebees

Cited by 32 publications

References 41 publications

Bifidobacterium actinocoloniiforme sp. nov. and Bifidobacterium bohemicum sp. nov., from the bumblebee digestive tract

Bifidobacterium actinocoloniiforme sp. nov. and Bifidobacterium bohemicum sp. nov., from the bumblebee digestive tract

Lactobacillus bombi sp. nov., from the digestive tract of laboratory-reared bumblebee queens (Bombus terrestris)

Comparative genomics of Bifidobacterium species isolated from marmosets and humans

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