Bifidin I – A new bacteriocin produced by Bifidobacterium infantis BCRC 14602: Purification and partial amino acid sequence

Produção de bacteriocina por Bifidobacterium lactis a partir de soro de leite

“…Ultimately, despite the many reports on Bifidobacterium-associated bacteriocins, bifidocin B from B. bifidum NCFB 1454 (Yildirim et al, 1999), bifidin I from Bifidobacterium infantis BCRC 14602 (partially sequenced) (Cheikhyoussef et al, 2010) and the lantibiotic bisin from B. longum DJO10A are the only bacteriocins that were in-depth characterized and whose amino acid sequence was at least partially elucidated. The (predicted) amino acid sequence of these bacteriocins is shown in Table 2.…”

“…These bacteriocins share a conserved sequence motif in their N-terminal region consisting of Tyr-Gly-Asn-Gly-Val-X-Cys-XXXXVal-X-Val (or YGNGVXCXXXXVXV, with X being any amino acid), which is stabilized by two cysteines forming a disulfide bridge, and a hydrophobic and/or amphiphilic C-terminal part consisting of one or two α-helices (Eijsink et al, 2002;von Ah, 2006). Bifidocin B from B. bifidum NCFB 1454 belongs to this class of bacteriocins having a N-terminal domain containing a Tyr-Gly-Asn-Gly-Val-X-Cys motif (Yildirim et al, 1999), while bifidin I from B. infantis BCRC 146, whose N-terminal domain although not completely sequenced contains a similar, but distinct Tyr-Gly-Asp-Val stretch (Cheikhyoussef et al, 2010), has its classification still under consideration (Table 2).…”

“…In addition to peptides (b 10 kDa), there are some reports on larger antimicrobial proteins (bifilact Bb-46, bifilact Bb-12 and bifilong) (Cheikhyoussef et al, 2010;Collado et al, 2005a,b) that are potentially belonging to the bacteriolysin family of antimicrobials (Cotter et al, 2005b).…”

“…Such a process allowed obtaining a final preparation with specific activity of 31,605 AU/mg, corresponding to overall 120-fold purification and 64% yield (Cheikhyoussef et al, 2009a). A new method has recently been developed by Cheikhyoussef et al (2010), whereby bifidin I was purified/ concentrated by a three-step process. The purification protocol started with its recovery by adsorption/desorption onto/from silicic acid, which resulted in a preparation with specific activity of 67,696 AU/mg, corresponding to 257-fold concentration and 80% yield.…”

“…Among the many probiotic traits that have been attributed to bifidobacteria are a) the induction of immunoglobulin production, b) improvement of food nutritional value by assimilation of substrates not metabolized by the host, c) anti-carcinogenic activity and d) folic acid synthesis (Bevilacqua et al, 2003;Cheikhyoussef et al, 2009a;Collado et al, 2005a;Gomes and Malcata, 1999;Touré et al, 2003). Interestingly for the purposes of this review, some bifidobacteria are also known to produce antimicrobials (Cheikhyoussef et al, 2009b;Gibson and Wang, 1994b;Gomes and Malcata, 1999;Ibrahim and Salameh, 2001) and, more specifically, bacteriocins (Anand et al, 1984(Anand et al, , 1985Cheikhyoussef et al, 2010;von Ah, 2006;Yildirim and Johnson, 1998;Yildirim et al, 1999).…”