Bienzymatic glucose biosensor based on co-immobilization of peroxidase and glucose oxidase on a carbon nanotubes electrode

Zhu, Lihua; Yang, Rui; Zhai, Jiangli; Tian, Chunyuan

doi:10.1016/j.bios.2007.07.002

Cited by 172 publications

(57 citation statements)

References 53 publications

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“…Nafion) [12], or selective catalysts (e.g. porphyrin [13], phthalocyanine [14], and metallic particles [17,18]) to diminish the over potential for the anodic oxidation of hydrogen peroxide.…”

Section: Pd/rgo/gce Towards Hydrogenmentioning

confidence: 99%

“…Especially, amperometric biosensor based on glucose oxidase (GOD) is extremely popular due to its simplicity, reliability, low cost and very stable activity [6][7][8][9]. In this type, glucose level is indirectly monitored by measurement of current associated with the electrochemical detection of hydrogen peroxide produced during the enzymatic reaction in which glucose is oxidized by oxygen with the aid of GOD.…”

Section: Introductionmentioning

confidence: 99%

“…Nafion) [10], or incorporation of redox mediators (e.g. porphyrin [11], phthalocyanine [12], Prussian blue [13], and peroxidase [14]) to diminish the over potential for the detection of hydrogen peroxide. Additionally, the oxidation of hydrogen peroxide can be promoted by some precious metal nanoparticles thanks to their large active surface area and favorable lattice planes compare to the bulks, such as Pt and Au [15,16].…”

Section: Introductionmentioning

confidence: 99%

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Amperometric Glucose Biosensor Based on Integration of Glucose Oxidase with Palladium Nanoparticles/Reduced Graphene Oxide Nanocomposite

Nana¹,

Wang²,

Li³

et al. 2012

AJAC

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We report on a new type of amperometric glucose biosensor that was made by integration of glucose oxidase (GOD) with palladium nanoparticles/reduce graphene oxide (Pd/RGO) nanocomposite. The Pd/RGO was prepared by a onestep reduction method in which the palladium nanoparticles and the reduced graphene oxide (RGO) were simultaneously accomplished from the reduction of dispersed solution of PdCl 2 and graphite oxide (GO) with hydrazine. The as-prepared nanocomposite exhibits favorable electrocatalytic activities towards the oxidation of H 2 O 2 , which makes it a good platform for the construction of the glucose biosensor. The analytical performance of the glucose biosensor is fully evaluated. It shows good analytical properties in terms of a short response time (3 s), high sensitivity (14.1 µA/mM), and low detection limit (0.034 mM). In addition, the effects of pH value, applied potential, electroactive interference and the stability of the biosensor were discussed as well.

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Section: Pd/rgo/gce Towards Hydrogenmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Amperometric Glucose Biosensor Based on Integration of Glucose Oxidase with Palladium Nanoparticles/Reduced Graphene Oxide Nanocomposite

Nana¹,

Wang²,

Li³

et al. 2012

AJAC

Self Cite

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“…A bienzymatic glucose biosensor was proposed for the selective and sensitive detection of glucose [91,92]. This mediatorless biosensor was made by the simultaneous immobilization of GOD and HRP in an electropolymerized pyrrole film on SWCNT-coated electrode.…”

Section: Glucosementioning

confidence: 99%

Electrochemical Biosensing Based on Carbon Nanotubes

Zhang

Wang

2011

NanoBiosensing

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“…Therefore increased effective reacting area is one way to obtain a higher sensitivity. Different research groups apply many different techniques to increase the effective surface area including the use of materials like carbon nanotubes and processes like nano-templating [10,[13][14][15][16][17]. In this research, we have investigated developing a nano-scale corrugated conductive polymer surface suitable for immobilizing glucose oxidase (GO x ) for the development of a low-cost nano-biosensor.…”

Section: Introductionmentioning

confidence: 99%

Functional design of electrolytic biosensor

Preethichandra

Ekanayake

Onoda

2017

J. Phys.: Conf. Ser.

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Abstract.A novel amperometric biosensbased on conjugated polypyrrole (PPy) deposited on a Pt modified ITO (indium tin oxide) conductive glass substrate and their performances are described. We have presented a method of developing a highly sensitive and low-cost nanobiosensor for blood glucose measurements. The fabrication method proposed decreases the cost of production significantly as the amount of noble metals used is minimized. A nanocorrugated PPy substrate was developed through pulsed electrochemical deposition. The sensitivity achieved was 325 mA/(Mcm 2 ) and the linear range of the developed sensor was 50-60 mmol/l. Then the application of the electrophoresis helps the glucose oxidase (GO x ) on the PPy substrate. The main reason behind this high enzyme loading is the high electric field applied across the sensor surface (working electrode) and the counter electrode where that pushes the nano-scale enzyme particles floating in the phosphate buffer solution towards the substrate. The novel technique used has provided an extremely high sensitivities and very high linear ranges for enzyme (GO x ) and therefore can be concluded that this is a very good technique to load enzyme onto the conducting polymer substrates.

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Bienzymatic glucose biosensor based on co-immobilization of peroxidase and glucose oxidase on a carbon nanotubes electrode

Cited by 172 publications

References 53 publications

Amperometric Glucose Biosensor Based on Integration of Glucose Oxidase with Palladium Nanoparticles/Reduced Graphene Oxide Nanocomposite

Amperometric Glucose Biosensor Based on Integration of Glucose Oxidase with Palladium Nanoparticles/Reduced Graphene Oxide Nanocomposite

Electrochemical Biosensing Based on Carbon Nanotubes

Functional design of electrolytic biosensor

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