Biennial review of planar chromatography: 2011–2013

Sherma, Joseph

doi:10.2478/s11532-013-0402-5

Cited by 15 publications

(7 citation statements)

References 224 publications

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“…Among the various approaches of liquid chromatography, TLC is known for its certain advantages, such as low cost, simplicity of the method and ease of optimization for successful resolution along with facility to photograph the chromatogram as clearly visible evidence of separation. Sherma (, ) reviewed the literature on application of TLC for enantioresolution in his regular biennial reviews along with advantages of modern TLC in pharmaceutical and drug analysis, comparing with HPLC and HPTLC as well. Application of TLC in enantiomeric analysis has been described in the literature (Bhushan & Martens, ; Martens, Günther, & Schickedanz, 1986; Kowalska & Sherma, ).…”

Section: Enantioresolution By Tlcmentioning

confidence: 99%

Bioassay, determination and separation of enantiomers of atenolol by direct and indirect approaches using liquid chromatography: A review

Batra

Bhushan

2017

Biomedical Chromatography

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Atenolol, a β-adrenergic receptor antagonist, is a chiral compound used for the treatment of cardiovascular diseases and to treat hypertension, coronary heart disease, arrhythmias, sinus tachycardia and myocardial infarction, where it acts preferentially upon the β-adrenergic receptors in the heart. It is marketed as a racemate, but only the (S)-enantiomer of (RS)-atenolol is responsible for the β-adrenoceptor blocking activity. Different chromatographic methods have been applied for the separation and determination of enantiomers. In this article a review is presented on liquid chromatographic methods for enantioseparation of (RS)-atenolol by both direct and indirect approaches involving practical applications of several chiral stationary phases, chiral derivatization reagents and ligand exchange and impregnation methods. These include methods using both HPLC and TLC for separation, determination and bioassay of enantiomers of atenolol. In addition, some aspects of enantioseparation under achiral phases of liquid chromatography have been briefly mentioned as applicable to (RS)-atenolol. This review provides current available enantioseparation choices not only for (RS)-atenolol but also for other applicable racemic drugs.

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Section: Enantioresolution By Tlcmentioning

confidence: 99%

Bioassay, determination and separation of enantiomers of atenolol by direct and indirect approaches using liquid chromatography: A review

Batra

Bhushan

2017

Biomedical Chromatography

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“…The versatility of TLC results from a wide variety of stationary phases used for separation. The common stationary phases include silica with different physical (e.g., pore diameter) and chemical traits (e.g., silanized silica, C n alkyl-bonded silica) as well as other sorbents such as celluloses, aluminas, and polyamides [6][7][8][9]. On the other hand, the improvement of separation of specific metabolites could be largely done by varying the mobile phases from pure nonpolar solvents to complex mixtures of solvents with different polarities [7][8][9].…”

Section: Tlc Analysis Of Small Molecules 21 Generalitiesmentioning

confidence: 99%

“…An elementary and simple approach for quantitative purpose is the visual comparison of the spot/band intensity of a known sample aliquot with the intensities of a concentration series of known standards, all developed in the same TLC plate. This approach offers semiquantitative results, with precision (expressed as absolute deviation) and accuracy (expressed as experimental error) ranged from 10 to 30% [6][7][8][9]. Concentration estimation via visual comparison depends on the interpretation of the analyst.…”

Section: Rf =mentioning

confidence: 99%

Chemometrics-Based TLC and GC-MS for Small Molecule Analysis: A Practical Guide

Vázquez-Martínez¹,

López²

2019

Chemometrics and Data Analysis in Chromatography

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Nowadays, thin-layer chromatography (TLC) and gas chromatography/mass spectrometry (GC-MS) instruments can produce more data than even before. At this point, the use of mathematical and statistical tools has provided the key to resolve the information overload. In this chapter, a practical guide is provided for the TLC and GC-MS analysis of short-chain fatty acids (SCFAs), amino acids, and monosaccharides. A methodology for extracting and transforming the chromatographic data to a suitable format for chemometrics is described. Furthermore, a procedure for chemometric analysis based on principal components analysis and clustering analysis is suggested.

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“…Developed planar chromatograms may be easily photographed, and this form of data can be simply transmitted via internet connections to given research centers. Moreover, planar chromatography can be easily miniaturized and hyphenated with advanced and sensitive detectors, including mass spectrometers, FTIR spectroscopy or electrochemical sensing devices [ 3 , 4 ]. It should be noted that planar chromatography is fairly efficient as a separation tool with several advances on their counterpart: column liquid chromatography (high-performance liquid chromatography; HPLC).…”

Section: Introductionmentioning

confidence: 99%

Long-Term Fluorescence Behavior of CdSe/ZnS Quantum Dots on Various Planar Chromatographic Stationary Phases

Zarzycki¹

2022

Nanomaterials

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Nanoparticles, particularly quantum dots (QDs), are commonly used for the sensitive detection of various objects. A number of target molecules may be determined using QDs sensing systems. Depending on their chemical nature, physicochemical properties, and spatial arrangement, QDs can selectively interact with given molecules of interest. This can be performed in complex systems, including microorganisms or tissues. Efficient fluorescence enables low exposure of QDs and high sensitivity for detection. One disadvantage of quantum dots fluorophores is fluorescence decay. However, for given applications, this property may be an advantage, e.g., for highly sensitive detection based on correlation images in the time domain. This experimental work deals with the measurement of fluorescence decay of Lumidot TMCdSe/ZnS (530 nm) quantum dots. These nanoparticles were transferred to the surface of various planar chromatographic stationary phases. Fluorescence of formed spots was recorded at room temperature over a long period of time, namely 15.7824 × 105 min (three years). The resulting signal profiles in the time domain were analyzed using classical approach (luminescence model comparison involving different mathematical models).Moreover, fluorescence behavior on different TLC/HPTLC supports was investigated using multivariate statistics (principal component analysis, PCA). Eight planar chromatographic stationary phases were investigated, including cellulose, octadecylsilane, polyamide, silica gel and aluminium oxide in different forms (TLC and HPTLC types). The presented research revealed significantly different and non-linear long-term QDs behavior on these solids. Two different fluorescence signal trajectories were recorded, including typical signal decay after QDs application to the plates and long-term intensity increase. This was particularly visible for given planar chromatographic adsorbents, e.g., cellulose or octadecylsilane. To the author’s knowledge, these findings were not reported before using the stationary chromatographic phases, and enable the design of future experiments toward sensing of low molecular mass chemicals using, e.g., advanced quantification approaches. This may include signal processing computations based on correlation images in the time domain. Additionally, the reported preliminary data indicates that the investigated nanoparticles can be applied as efficient and selective fluorophores. This was demonstrated on micro-TLC plates where separated bioactive organic substances quenching from cyanobacteria extracts were sensitively detected. The described detection protocol can be directly applied for different planar chromatographic systems, including paper-based microfluidic devices, planar electrophoresis and/or miniaturized microfluidic chip devices.

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Biennial review of planar chromatography: 2011–2013

Cited by 15 publications

References 224 publications

Bioassay, determination and separation of enantiomers of atenolol by direct and indirect approaches using liquid chromatography: A review

Bioassay, determination and separation of enantiomers of atenolol by direct and indirect approaches using liquid chromatography: A review

Chemometrics-Based TLC and GC-MS for Small Molecule Analysis: A Practical Guide

Long-Term Fluorescence Behavior of CdSe/ZnS Quantum Dots on Various Planar Chromatographic Stationary Phases

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