Bidirectional myofiber transition through altering the photobiomodulation condition

Cao, Lin; Niwa, Akira; Hou, Chun Yin; Tsai, Chung Min; Chang, Hsi

doi:10.1016/j.jphotobiol.2020.112041

Cited by 4 publications

(2 citation statements)

References 38 publications

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“…PBMT has been reported to exert a beneficial influence on muscle regeneration. This is achieved through its capacity to stimulate the proliferation of muscle and satellite cells, enhancing protein synthesis in myoblasts, increasing the area of muscle fibers, enhance mitochondrial density [14][15][16][17], and upregulate the expression of myogenic regulatory factor, including pax7, myoD, and myogenin [15,[18][19].…”

Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production

Silva,

Gouveia,

Campos

et al. 2024

PLoS Negl Trop Dis

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Background Photobiomodulation has exhibited promise in mitigating the local effects induced by Bothrops snakebite envenoming; however, the mechanisms underlying this protection are not yet fully understood. Herein, the effectiveness of photobiomodulation effects on regenerative response of C2C12 myoblast cells following exposure to Bothrops jararacussu venom (BjsuV), as well as the mechanisms involved was investigated. Methodology/Principal findings C2C12 myoblast cells were exposed to BjsuV (12.5 μg/mL) and irradiated once for 10 seconds with laser light of 660 nm (14.08 mW; 0.04 cm2; 352 mW/cm2) or 780 nm (17.6 mW; 0.04 cm2; 440 mW/ cm2) to provide energy densities of 3.52 and 4.4 J/cm2, and total energies of 0.1408 and 0.176 J, respectively. Cell migration was assessed through a wound-healing assay. The expression of MAPK p38-α, NF-Кβ, Myf5, Pax-7, MyoD, and myogenin proteins were assessed by western blotting analysis. In addition, interleukin IL1-β, IL-6, TNF-alfa and IL-10 levels were measured in the supernatant by ELISA. The PBM applied to C2C12 cells exposed to BjsuV promoted cell migration, increase the expression of myogenic factors (Pax7, MyF5, MyoD and myogenin), reduced the levels of proinflammatory cytokines, IL1-β, IL-6, TNF-alfa, and increased the levels of anti-inflammatory cytokine IL-10. In addition, PBM downregulates the expression of NF-kB, and had no effect on p38 MAKP. Conclusion/Significance These data demonstrated that protection of the muscle cell by PBM seems to be related to the increase of myogenic factors as well as the modulation of inflammatory mediators. PBM therapy may offer a new therapeutic strategy to address the local effects of snakebite envenoming by promoting muscle regeneration and reducing the inflammatory process.

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Section: Plos Neglected Tropical Diseasesmentioning

confidence: 99%

Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production

Silva,

Gouveia,

Campos

et al. 2024

PLoS Negl Trop Dis

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“…The maximum weight the experimental rat lifted was measured, with the maximum weight defined as when the rat failed to lift the given task three times continuously. The TLT was first introduced in our previous study (Kou et al 2019;Lin et al 2020). In this method, experimental animal sacrifice is not required, and hence, sequential daily evaluation is possible.…”

Section: Introductionmentioning

confidence: 99%

Toe-lift test: a novel, simple and noninvasive in vivo method for contractile evaluation of tibialis anterior muscle

Tsai¹,

Lo²,

Lin³

et al. 2022

gpb

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Tibialis anterior (TA) muscle has frequently been used for scientific experiments, particularly for muscle contractile assays, because of its anatomical advantages. However, classical evaluation methods for the TA muscle, such as EMG and force transducer, require experimental skills to acquire reliable results. Furthermore, because sacrificing experimental animals is usually indispensable for both methods, sequential observations cannot be performed. Therefore, developing a simple, objective, and animal friendly evaluation system was warranted. In this article, we introduce a novel, simple, and noninvasive in vivo evaluation method for the TA muscle called the toe-lift test (TLT), which is not only easy to perform but also capable of detecting contractile strength precisely. Because the TLT does not require experimental animal sacrifice, performing assessments over time, such as in sequential observation, is possible. This novel method represents a solution to the need for a simple, noninvasive, and effective method for TA muscle contractile evaluation.

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Photobiomodulation therapy on skeletal muscles exposed to diabetes mellitus: a systematic review of animal studies

dos Santos Soares,

de Souza Pinto,

Kruger

et al. 2023

Lasers Med Sci

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Bidirectional myofiber transition through altering the photobiomodulation condition

Cited by 4 publications

References 38 publications

Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production

Photobiomodulation mitigates Bothrops jararacussu venom-induced damage in myoblast cells by enhancing myogenic factors and reducing cytokine production

Toe-lift test: a novel, simple and noninvasive in vivo method for contractile evaluation of tibialis anterior muscle

Photobiomodulation therapy on skeletal muscles exposed to diabetes mellitus: a systematic review of animal studies

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