Bidirectional mechanobiology between cells and their local extracellular matrix probed by atomic force microscopy

Alcaraz, Jordi; Otero, J. F. Fuentes; Jorba, Ignasi; Navajas, Daniel

doi:10.1016/j.semcdb.2017.07.020

Cited by 68 publications

(73 citation statements)

References 64 publications

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“…Métodos de informática semi-automatizados de segmentação de cores, por exemplo, permitem a obtenção de dados estereológicos baseados nos diferentes espectros de pixels (AMENÁBAR et al, 2006;JONES, 2013;SARAVANAN et al, 2017;RODRIGUES, 2018). Complementarmente à estereologia, a AFM possui relevante potencial de empregabilidade na dermatologia veterinária por permitir a obtenção de imagens topográficas com resolução atômica, por meio do contato intermitente da sonda de varredura com a amostra (TANG, 2010;ALCARAZ et al, 2018). Além disso, pode caracterizar as propriedades biomecânicas da epiderme e derme, como a rigidez, por exemplo, pelo módulo de Young (FERREIRA, 2006;PAYDAR, 2016).…”

Section: Desenvolvimentounclassified

“…Essa técnica deve ser adaptada ao tecido cutâneo, por meio da calibração precisa do microscópio de AFM, a fim de evitar danos celulares induzidos pelas forças de adesão do tecido à sonda de AFM (BUTT, 2005;ALCARAZ et al, 2018).…”

Section: Desenvolvimentounclassified

“…Nesse contexto, a AFM destaca-se por ser a primeira técnica de microscopia avançada para dimensões nanométricas e subnanométrica, fornecendo mapas topográficos precisos de resolução atômica (FERREIRA, 2006;BUTT, 2005;ALCARAZ et al, 2018).…”

Section: Desenvolvimentounclassified

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Aplicabilidade da estereologia e microscopia de força atômica à pesquisa dermatológica veterinária (revisão de literatura)

Barbosa

Melo

Rodrigues³

et al. 2020

J.Interdisc.Bio.

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Em dermatologia veterinária, persiste a tradição morfológica de obtenção de dados essencialmente qualitativos. A estereologia e a microscopia de força atômica são técnicas que combinam estatística e microscopia avançada para obter informações quantitativas tridimensionais, como volume total, volume ultraestrutural, densidade volumétrica, número total de estruturas, dentre outras, a partir de imagens bidimensionais. Essas técnicas são cada vez mais relevantes para determinar a heterogeneidade morfológica comumente encontrada em amostras teciduais. A utilização concomitante dessas técnicas favorece a obtenção de dados matemáticos e físicos, os quais são mais fidedignos e de maior acurácia que dados morfológicos qualitativos, baseados em descrições morfométricas bidimensionais. Este trabalho consiste de uma pesquisa de natureza descritiva, na modalidade de pesquisa bibliográfica, a respeito do uso das técnicas de delineamento estereológico e microscopia de força atômica. Para tanto, foram selecionados artigos em inglês e português, de 2003 a 2019, a partir de bases de dados online (PUBMED, SCIELO). A estereologia e microscopias avançadas, como a microscopia de força atômica, são altamente relevantes pela capacidade de fornecimento de dados sofisticados e fidedignos na área de interesse, tornando úteis para práticas morfodescritivas veterinárias.

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Section: Desenvolvimentounclassified

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Aplicabilidade da estereologia e microscopia de força atômica à pesquisa dermatológica veterinária (revisão de literatura)

Barbosa

Melo

Rodrigues³

et al. 2020

J.Interdisc.Bio.

View full text Add to dashboard Cite

show abstract

“…Within the tissues, the maintaining of their structure and function is guaranteed also by cell adhesion. This activity is accomplished by the focal adhesion proteins, a large complex that assemblies through mechanical force and regulatory signals and connects the extracellular matrix with the cell [136][137][138][139][140][141]. The molecular adhesion of the cells on a biomaterial can be studied by monitoring the focal adhesion proteins such as Cadherins and Vinculin.…”

Section: Cell Adhesionmentioning

confidence: 99%

Recent Advances in Nanocomposites Based on Aliphatic Polyesters: Design, Synthesis, and Applications in Regenerative Medicine

et al. 2018

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In the last decade, biopolymer matrices reinforced with nanofillers have attracted great research efforts thanks to the synergistic characteristics derived from the combination of these two components. In this framework, this review focuses on the fundamental principles and recent progress in the field of aliphatic polyester-based nanocomposites for regenerative medicine applications. Traditional and emerging polymer nanocomposites are described in terms of polymer matrix properties and synthesis methods, used nanofillers, and nanocomposite processing and properties. Special attention has been paid to the most recent nanocomposite systems developed by combining alternative copolymerization strategies with specific nanoparticles. Thermal, electrical, biodegradation, and surface properties have been illustrated and correlated with the nanoparticle kind, content, and shape. Finally, cell-polymer (nanocomposite) interactions have been described by reviewing analysis methodologies such as primary and stem cell viability, adhesion, morphology, and differentiation processes.

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“…A number of research, performed on cells, has shown that cancer cells tend to be more deformable in comparison with normal ones . The most recent studies have shown that AFM tissue examination may be also successfully applied in cancer diagnosis . One of the main AFM advantages is a small sample volume as all measurements are performed in nanoscale and a capability of measurements in liquid conditions mimicking natural ones.…”

Section: Introductionmentioning

confidence: 99%

AFM‐based nanomechanical characterization of bronchoscopic samples in asthma patients

Zemła

Stachura

Gross‐Sondej

et al. 2018

J of Molecular Recognition

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Asthma is not a single disease, but recently, it is considered as a syndrome characterized through various clinical presentations and different etiopathologies. Large degree of the disease heterogeneity manifests in distinct characteristics that translate into variability of properties at single cell and molecular levels. Here, we conducted measurements of mechanical properties of bronchial tissue samples collected from patients suffering from asthma. The results obtained from different applied protocols for sample preparation may indicate that deep freezing and storage in liquid nitrogen, followed by consecutive unfreezing of tissue samples, preserve tissue mechanical properties as indicated by a parameter referred here as a tissue relative stiffness index. Tissue relative stiffness index quantifies both the degree of heterogeneity and deformability of tissue samples regarding healthy one. These studies demonstrate that the freezing protocol, optimized towards asthma tissue, can facilitate atomic force microscopy use what, together with recent findings on standardization of elasticity measurements, enables the measurements of large group of samples with minimized influence of errors stemming from the applied methodology of tissue stiffness determination.

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Bidirectional mechanobiology between cells and their local extracellular matrix probed by atomic force microscopy

Cited by 68 publications

References 64 publications

Aplicabilidade da estereologia e microscopia de força atômica à pesquisa dermatológica veterinária (revisão de literatura)

Aplicabilidade da estereologia e microscopia de força atômica à pesquisa dermatológica veterinária (revisão de literatura)

Recent Advances in Nanocomposites Based on Aliphatic Polyesters: Design, Synthesis, and Applications in Regenerative Medicine

AFM‐based nanomechanical characterization of bronchoscopic samples in asthma patients

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