Bidirectional effect of Wnt signaling antagonist DKK1 on the modulation of anthrax toxin uptake

Qian, Lili; Cai, Changzu; Yuan, Peng; Jeong, Sun-Young; Yang, Xiaozhou; DeAlmeida, Venita; Ernst, James A.; Costa, Michael A.; Cohen, Stanley N.; Wei, Wensheng

doi:10.1007/s11427-014-4646-x

Cited by 11 publications

(13 citation statements)

References 61 publications

(88 reference statements)

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“…DKK1 is a secretory protein that was first verified to have regulatory effect on the development of embryonic head in xenopus laevis. Subsequently, DKK1 was supposed to have regulatory effect on Wnt/β-catenin signal pathway in human (16). Later, it is confirmed in numerous studies that DKK1 plays an important role in the genesis and metastasis mechanisms of tumor, and it is verified in some tumors to have value in predicting prognosis (17).…”

Section: Discussionmentioning

confidence: 92%

MicroRNA-152 inhibits cell proliferation of osteosarcoma by directly targeting Wnt/β-catenin signaling pathway in a DKK1-dependent manner

Zhao

Sun²,

et al. 2018

Oncol Rep

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The function of microRNA‑152 for a cohort of patients with osteosarcoma and the influence on cell growth of osteosarcoma were studied. The expression of microRNA‑152 was downregulated in patients with osteosarcoma. The downregulation of microRNA‑152 promotes cell proliferation, decreases apoptosis, and suppresses LDH activity, caspase-3/9 activities and Bax/Bcl-2 and p53 protein expression levels of osteosarcoma through activation of the Wnt/β-catenin signaling pathway by targeting DKK1. Whereas, overexpression of microRNA‑152 targets DKK1 to inhibit cell proliferation, induce apoptosis, and promote LDH activity, caspase-3/9 activities and Bax/Bcl-2 and p53 protein expression levels of osteosarcoma through inactivation of the Wnt/β-catenin signaling pathway. The promotion of DKK1 reversed the anticancer function of microRNA‑152 over-expression through Wnt/β-catenin signaling pathway. We demonstrated that microRNA‑152 inhibits cell proliferation of osteosarcoma through DKK1 by directly targeting Wnt/β‑catenin signaling pathway.

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Section: Discussionmentioning

confidence: 92%

MicroRNA-152 inhibits cell proliferation of osteosarcoma by directly targeting Wnt/β-catenin signaling pathway in a DKK1-dependent manner

Zhao

Sun²,

et al. 2018

Oncol Rep

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“…When distributed on the cell membrane, it also has an adhesion function but can additionally participate in autophagy, promote cell apoptosis, and recruit transferrin [43]. Adhesion, as the first step of bacterial infection, is of great significance to the invasive ability of bacteria and the effective use of toxins [44]. The results showed that the degree of expression in the biofilm state is higher than that in a planktonic state.…”

Section: Discussionmentioning

confidence: 99%

An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of Aeromonas veronii TH0426

Li¹,

Yang²,

Tian³

et al. 2020

IJMS

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Aeromonas veronii is a virulent fish pathogen that causes extensive economic losses in the aquaculture industry worldwide. In this study, a virulent strain of A. veronii TH0426 was used to establish an in vitro biofilm model. The results show that the biofilm-forming abilities of A. veronii TH0426 were similar in different media, peaking under conditions of 20 °C and pH 6. Further, isobaric tags for relative and absolute quantitation (iTRAQ)-based quantitative proteomics methods were used to compare the differential expression of A. veronii between the biofilm and planktonic cells. The results show alterations in 277 proteins, with 130 being upregulated and 147 downregulated. Pathway analysis and GO (Gene Ontology) annotations indicated that these proteins are mainly involved in metabolic pathways and the biosynthesis of secondary metabolites and antibiotics. These proteins are the main factors affecting the adaptability of A. veronii to its external environment. MRM (multiple reaction 27 monitoring) and qPCR (qPCR) were used to verify the differential proteins of the selected A. veronii. This is the first report on the biofilm and planktonic cells of A. veronii, thus contributing to studying the infection and pathogenesis of A. veronii.

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“…In Wnt signaling pathway, DKK1 can form ternary complex with LRP6 and Kremen2, another membrane protein, for internalization 12 13 . We have recently reported that DKK1 plays an important role in the internalization and lethality of anthrax toxin through the ternary structure of LRP6-DKK1-Kremen2 14 . However, it is still technically challenging to investigate the function of secreted protein in real time without toilsome protein production and purification processes.…”

mentioning

confidence: 99%

A microfluidic live cell assay to study anthrax toxin induced cell lethality assisted by conditioned medium

Shen

Cai

et al. 2015

Sci Rep

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It is technically challenging to investigate the function of secreted protein in real time by supply of conditioned medium that contains secreted protein of interest. The internalization of anthrax toxin is facilitated by a secreted protein Dickkopf-1 (DKK1) and its receptor, and eventually leads to cell lethality. To monitor the dynamic interplay between these components in live cells, we use an integrated microfluidic device to perform the cell viability assays with real-time controlled culture microenvironment in parallel. Conditioned medium, which contains the secreted proteins from specific cell lines, can be continuously pumped towards the cells that exposed to toxin. The exogenous DKK1 secreted from distant cells is able to rescue the sensitivity to toxin for those DKK1-knocked-down cells. This high-throughput assay allows us to precisely quantify the dynamic interaction between key components that cause cell death, and provide independent evidence of the function of DKK1 in the complex process of anthrax toxin internalization.

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Bidirectional effect of Wnt signaling antagonist DKK1 on the modulation of anthrax toxin uptake

Cited by 11 publications

References 61 publications

MicroRNA-152 inhibits cell proliferation of osteosarcoma by directly targeting Wnt/β-catenin signaling pathway in a DKK1-dependent manner

MicroRNA-152 inhibits cell proliferation of osteosarcoma by directly targeting Wnt/β-catenin signaling pathway in a DKK1-dependent manner

An iTRAQ-Based Comparative Proteomics Analysis of the Biofilm and Planktonic States of Aeromonas veronii TH0426

A microfluidic live cell assay to study anthrax toxin induced cell lethality assisted by conditioned medium

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