Bicarbonate Secretion in the Guinea Pig Duodenum: Functional Characterization of Peptide Hormone Receptors in Duodenal Enterocytes

Reimer, R.; Odes, H S; Beil, Winfried; Schwenk, Michael; Muallem, R; Sewing, Karl–Friedrich

doi:10.1159/000139400

Cited by 6 publications

(2 citation statements)

References 12 publications

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“…VIP and PGE2 were shown to act via receptors coupled to adenylate cyclase in iso lated enterocytes in the rat [23] and man [24], We have recently reported that VIP and PGE2 potently stimulate adenylate cyclase and protein kinase A in homogenized duodenal enterocytes, but secretin and PGF2(1 were only weak stimulants [21], This is consistent with the strong effects of PGE2 and VIP on duodenal bicarbonate secre tion in this study. We have recently described the pres ence on duodenal enterocytes of VIP receptors [25] and EP2 subtype receptors for PGE2 [15], All this is corrobora tive evidence for the role of cAMP in bicarbonate secre tion.…”

Section: Second Messengers O F Bicarbonate Secretionsupporting

confidence: 60%

Comparative Activities of Agonists of Active Duodenal Bicarbonate Secretion in the Guinea Pig

Odes

Muallem²,

Reimer³

et al. 1994

Digestion

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The comparative activity of agonists of duodenal bicarbonate secretion was studied in the anesthetized guinea pig, where the duodenal lumen was perfused with 24 mmol/l NaHCO₃ to ensure active secretion of bicarbonate. Agonists were infused alone and in combination. Dibutyryl 3’,5’-cyclic adenosine monophosphate, vasoactive intestinal polypeptide (VIP) and prostaglandin E₂ (PGE₂) were strong stimulants of bicarbonate secretion. Theophylline, dibutyryl 3’,5’-cyclic guanosine monophosphate, glucagon and prostaglandin F_2α (PGF_2α) were weaker agonists, and secretin had no effect. Combinations of any two of VIP, PGE₂ and glucagon depressed bicarbonate secretion, whereas combinations of PGE₂ and PGF_2α, VIP and PGE₂, and glucagon and PGF_2α increased bicarbonate secretion. The data indicate that cAMP and other secondary messengers may mediate duodenal bicarbonate secretion.

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Section: Second Messengers O F Bicarbonate Secretionsupporting

confidence: 60%

Comparative Activities of Agonists of Active Duodenal Bicarbonate Secretion in the Guinea Pig

Odes

Muallem²,

Reimer³

et al. 1994

Digestion

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“…VPAC 2 receptor mRNA has been identified in duodenal mucosa and epithelium [14]. Specific VIP receptors have been found on duodenocytes [41], and secretin does not stimulate these receptors. If, as indicated above, this VIP receptor is the VPAC 2 receptor, equal effects of PACAP27, PACAP38 and VIP on DMBS are to be expected.…”

Section: Discussionmentioning

confidence: 99%

Effect of Vasoactive Intestinal Peptide and Pituitary Adenylate Cyclase-Activating Polypeptide on Pancreatic, Hepatic and Duodenal Mucosal Bicarbonate Secretion in the Pig

Glad

Ainsworth²,

Svendsen³

et al. 2003

Digestion

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Background and Method: The potency of pituitary adenylate cyclase-activating polypeptide (PACAP) and the related vasoactive intestinal peptide (VIP) on pancreaticobiliary and duodenal mucosal bicarbonate secretion (DMBS) was studied in anaesthetized pigs. VIP, PACAP27 and PACAP38 were infused at doses of 250, 500, 1,000, 2,000 and 6,000 pmol/kg·h. Results: Infusion of VIP in these concentrations increased the portal plasma concentrations of VIP from 9 to 102, 220, 600, 850 and 3,795 pM, respectively. At doses of 1,000 and 6,000 pmol/kg·h all three peptides significantly stimulated pancreatic bicarbonate secretion, VIP from 0.0 to 0.3 and 7.6 mmol/h, PACAP27 from 0.0 to 2.3 and 8.2 mmol/h, and PACAP38 from 0.0 to 0.2 and 7.4 mmol/h, respectively. Only during infusion of 6,000 pmol/kg·h did the three peptides elicit a significant increase in hepatic bicarbonate output. At this dose VIP increased hepatic bicarbonate output from 0.4 to 3.8 mmol/h, PACAP27 from 0.3 to 3.6 mmol/h, and PACAP38 from 0.1 to 1.4 mmol/h, respectively. Infusion of VIP (500, 1,000, 2,000 and 6,000 pmol/kg·h) dose-dependently and significantly enlarged DMBS from 0.09 to 0.19, 0.41, 0.54 and 0.53 mmol/h, respectively. A significant effect of PACAP27 and PACAP38 on DMBS was only obtained at a dose of 6,000 pmol/kg·h, and at this dose VIP-induced DMBS was 4- and 8-fold higher than that induced by PACAP27 and PACAP38, respectively. Conclusions: VIP, PACAP27 and PACAP38 all have a pharmacological effect on both DMBS and pancreaticobiliary bicarbonate secretion. When comparing these results with the distribution of VIP/PACAP neurons and relevant receptors, it seems likely that VIP and PACAP have a physiological importance as neurotransmitters in the pancreas and duodenum, but not in the liver. The differences in the effect of VIP and PACAP on DMBS observed in this study indicate that there are variations between pancreatic and duodenal PACAP/VIP receptor subtypes, or that the duodenal inactivation of the peptides is different from the inactivation in the pancreas.

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Secretin: Should we revisit its metabolic outcomes?

St-Pierre

Broglio

2010

J Endocrinol Invest

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Metabolic pathologies such as Type 2 Diabetes have become a major health problem for worldwide populations. Unfortunately, efforts to cure and especially to prevent these significant global problems have so far been met with disappointment. Recently, the involvement of the gut-derived hormonal dysregulation in the development of obesity-related disturbances has been intensively studied. For instance, studies of gut-derived peptides such as peptide YY 3-36, glucagon-like peptide-1, oxyntomodulin and, more recently, ghrelin have significantly improved our understanding of mechanisms underlying weight and metabolic regulation. Even though early reports of the existence of secretin, the first peptide hormone to be described, date back as far as 1825, so much and yet so little is still known about its physiological role in mammals, including humans. However, recent years have provided a better understanding of how the release of secretin is regulated by enteral secretagogues. On the other hand, most basic questions about its role in the post-prandial regulation of metabolic functions in normal and pathophysiological conditions remain to be elucidated. The present work intends to review the physiology of secretin along with its central and peripheral outcomes on metabolic functions.

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Bicarbonate Secretion in the Guinea Pig Duodenum: Functional Characterization of Peptide Hormone Receptors in Duodenal Enterocytes

Cited by 6 publications

References 12 publications

Comparative Activities of Agonists of Active Duodenal Bicarbonate Secretion in the Guinea Pig

Comparative Activities of Agonists of Active Duodenal Bicarbonate Secretion in the Guinea Pig

Effect of Vasoactive Intestinal Peptide and Pituitary Adenylate Cyclase-Activating Polypeptide on Pancreatic, Hepatic and Duodenal Mucosal Bicarbonate Secretion in the Pig

Secretin: Should we revisit its metabolic outcomes?

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