Biaxial strength of multilaminated extracellular matrix scaffolds

Freytes, Donald O.; Badylak, Stephen F.; Webster, Thomas J.; Geddes, L. A.; Rundell, Ann E.

doi:10.1016/j.biomaterials.2003.09.015

Cited by 198 publications

(165 citation statements)

References 31 publications

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“…Well-defined bands characteristic of the collagen type-1 can be visualized in samples C, G and I (as digested with 20 g L -1 UBM and 1 g L -1 pepsin), which is in accordance with the ones reported in the literature [23,28]. After 6 h of digestion using 20 g L -1 UBM and 1 g L -1 of pepsin ( Fig.…”

Section: Ubm Characterizationsupporting

confidence: 90%

“…In fact, BC has shown to be an interesting and versatile biomaterial, particularly in tissueengineered applications (tissue scaffolds and drug delivery systems) for the regeneration of damaged or diseased tissues and organs [19][20][21]. Also, UBM has been widely studied in several pre-clinical studies as a biologic scaffold for the reconstruction of damaged tissues [22][23][24][25][26][27][28].…”

Section: Introductionmentioning

confidence: 99%

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Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

Gonçalves

Rodrigues

Padrão

et al. 2016

Colloids and Surfaces B: Biointerfaces

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This work evaluated the effect of acetylated bacterial cellulose (ABC) substrates coated with urinary bladder matrix (UBM) on the behavior of retinal pigment epithelium (RPE), as assessed by cell adhesion, proliferation and development of cell polarity exhibiting transepithelial resistance and polygonal shaped-cells with microvilli. Acetylation of bacterial cellulose (BC) generated a moderate hydrophobic surface (around 65°) while the adsorption of UBM onto these acetylated substrates did not affect significantly the surface hydrophobicity. The ABS substrates coated with UBM enabled the development of a cell phenotype closer to that of native RPE cells. These cells were able to express proteins essential for their cytoskeletal organization and metabolic function (ZO-1 and RPE65), while showing a polygonal shaped morphology with microvilli and a monolayer configuration. The coated ABC substrates were also characterized, exhibiting low swelling effect (between 1.5-2.0 swelling/mm3), high mechanical strength (2048MPa) and non-pyrogenicity (2.12EU/L). Therefore, the ABC substrates coated with UBM exhibit interesting features as potential cell carriers in RPE transplantation that ought to be further explored. Therefore, the ABC substrates coated with UBM showed a great potential to be applied as cell carriers in RPE transplantation.

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Section: Ubm Characterizationsupporting

confidence: 90%

Section: Introductionmentioning

confidence: 99%

Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

Gonçalves

Rodrigues

Padrão

et al. 2016

Colloids and Surfaces B: Biointerfaces

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show abstract

“…30 Layering of materials is generally thought to increase the force required to cause mechanical failure. 37 Interestingly, in the current study, the use of four-layer SIS did not have any physical advantage over one layer SIS, indicating that a single layer is adequate for reinforcing the staple line. The hypothesized reason for this is that each layer of the layered material perform independently, therefore they do not act as a single unit to improve the overall mechanical strength when used for this purpose.…”

Section: Discussioncontrasting

confidence: 44%

Buttressing Staples with Cholecyst-derived Extracellular Matrix (CEM) Reinforces Staple Lines in an Ex Vivo Peristaltic Inflation Model

et al. 2008

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“…Porcine urinary bladders were harvested from euthanized market weight (240-260 lb) pigs. The basement membrane and underlying lamina propria were isolated and harvested as described (58). After peracetic acid, ethanol, deionized H 2 O, and PBS treatment (19), lyophilized sheets were comminuted and digested in pepsin and 0.01 M HCl for 48 h before neutralization and dilution in PBS to yield a 5 mg/ mL solution.…”

Section: Methodsmentioning

confidence: 99%

Epimorphic regeneration approach to tissue replacement in adult mammals

Agrawal

Johnson²,

Reing³

et al. 2009

Proc. Natl. Acad. Sci. U.S.A.

141

134

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Urodeles and fetal mammals are capable of impressive epimorphic regeneration in a variety of tissues, whereas the typical default response to injury in adult mammals consists of inflammation and scar tissue formation. One component of epimorphic regeneration is the recruitment of resident progenitor and stem cells to a site of injury. Bioactive molecules resulting from degradation of extracellular matrix (ECM) have been shown to recruit a variety of progenitor and stem cells in vitro in adult mammals. The ability to recruit multipotential cells to the site of injury by in vivo administration of chemotactic ECM degradation products in a mammalian model of digit amputation was investigated in the present study. Adult, 6- to 8-week-old C57/BL6 mice were subjected to midsecond phalanx amputation of the third digit of the right hind foot and either treated with chemotactic ECM degradation products or left untreated. At 14 days after amputation, mice treated with ECM degradation products showed an accumulation of heterogeneous cells that expressed markers of multipotency, including Sox2, Sca1, and Rex1 (Zfp42). Cells isolated from the site of amputation were capable of differentiation along neuroectodermal and mesodermal lineages, whereas cells isolated from control mice were capable of differentiation along only mesodermal lineages. The present findings demonstrate the recruitment of endogenous stem cells to a site of injury, and/or their generation/proliferation therein, in response to ECM degradation products.

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Biaxial strength of multilaminated extracellular matrix scaffolds

Cited by 198 publications

References 31 publications

Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

Acetylated bacterial cellulose coated with urinary bladder matrix as a substrate for retinal pigment epithelium

Buttressing Staples with Cholecyst-derived Extracellular Matrix (CEM) Reinforces Staple Lines in an Ex Vivo Peristaltic Inflation Model

Epimorphic regeneration approach to tissue replacement in adult mammals

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