“…In this study, we show that a genetic perturbation of the excitatory glutamate system leads to profound functional alterations in neuronal and astroglial networks, leading to spontaneous and light-induced seizures. Our results are in line with the earlier observations associating EAAT2 malfunctions to seizures in mice and humans (Epi4K Consortium et al, 2013;Epi4K Consortium, 2016;Guella et al, 2017;Petr et al, 2015;Stergachis et al, 2019;Tanaka et al, 1997;Wagner et al, 2018), and with findings on abnormal motor behavior in the zebrafish techno trousers (tnt) mutant defective in EAAT2a (McKeown et al, 2012). Firstly, homozygous mutations of the astroglial eaat2a in zebrafish resemble a form of DEE present in human epilepsy patients with de novo mutations in the orthologous gene (EAAT2 = SLC1A2) (Epi4K Consortium et al, 2013;Epi4K Consortium, 2016;Guella et al, 2017).…”