bHLH142 regulates various metabolic pathway-related genes to affect pollen development and anther dehiscence in rice

Ranjan, Rajeev; Khurana, Ritika; Malik, Naveen; Badoni, Saurabh; Parida, Swarup K.; Kapoor, Sanjay; Tyagi, Akhilesh K.

doi:10.1038/srep43397

Cited by 39 publications

(33 citation statements)

References 57 publications

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“…Severe glume developmental defects can lead to infertility in rice (Xiao et al, 2009;Hong et al, 2010). Similarly, pollination failure due to anther indehiscence leads directly to sterility (Thangasamy et al, 2011;Zhou et al, 2011;Ranjan et al, 2017).…”

Section: Introductionmentioning

confidence: 99%

Ornithine δ‐aminotransferase is critical for floret development and seed setting through mediating nitrogen reutilization in rice

et al. 2018

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Nitrogen is one of the most important nutrient element that is essential for plant growth and development. Many genes have been reported to contribute to nitrogen absorption and transportation. However, genes involved in nitrogen reutilization are seldom reported. Ornithine δ-aminotransferase (δOAT) is the enzyme connecting arginine cycling and proline cycling. Here, we found that OsOAT, the homologue of δOAT in rice, is essential for nitrogen reutilization through mediating arginase activity. In the Osoat mutant, metabolic abnormality induced by nitrogen deficiency in floret causes malformed glumes, incapable glume opening and anther indehiscence. These defects in the mutant affect the pollination process and lead to a low seed setting rate as well as abnormal seed shape. Intriguingly, urea can rescue the phenotypes of the Osoat mutant. Therefore, OsOAT is crucial for nitrogen reutilization and plays a critical role in floret development and seed setting in rice.

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Section: Introductionmentioning

confidence: 99%

Ornithine δ‐aminotransferase is critical for floret development and seed setting through mediating nitrogen reutilization in rice

et al. 2018

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“…We found significant upregulation of UDT1 , GAMYB , TDR , and EAT1 at the early stage in OE142 anthers. In contrast, no alternation in expression of these master regulators was detected in the RNA-seq data presented by Ranjan et al (2017). Another potential shortfall of their study is the pollen development stages used for transcriptomic profiling.…”

Section: Discussionmentioning

confidence: 87%

“…For example, the known pollen wall synthesis marker genes such as OsC4 , LTP45 , CYP703A3 , and C6 were upregulated but CYP704B2 was downregulated in their OE line (Ranjan et al, 2017, their Table 1). This is contradictory, as it has been clearly shown that in the pollen development regulatory network bHLH142 is located upstream of Cyp703A3 , Cyp704B2 , MS2 , and C6 and they are involved in the same sporopollenin biosynthesis pathway (Aya et al, 2011; Ko et al, 2014).…”

Section: Discussionmentioning

confidence: 99%

Tightly Controlled Expression of bHLH142 Is Essential for Timely Tapetal Programmed Cell Death and Pollen Development in Rice

Lin

et al. 2017

Front. Plant Sci.

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Male sterility is important for hybrid seed production. Pollen development is regulated by a complex network. We previously showed that knockout of bHLH142 in rice (Oryza sativa) causes pollen sterility by interrupting tapetal programmed cell death (PCD) and bHLH142 coordinates with TDR to modulate the expression of EAT1. In this study, we demonstrated that overexpression of bHLH142 (OE142) under the control of the ubiquitin promoter also leads to male sterility in rice by triggering the premature onset of PCD. Protein of bHLH142 was found to accumulate specifically in the OE142 anthers. Overexpression of bHLH142 induced early expression of several key regulatory transcription factors in pollen development. In particular, the upregulation of EAT1 at the early stage of pollen development promoted premature PCD in the OE142 anthers, while its downregulation at the late stage impaired pollen development by suppressing genes involved in pollen wall biosynthesis, ROS scavenging and PCD. Collectively, these events led to male sterility in OE142. Analyses of related mutants further revealed the hierarchy of the pollen development regulatory gene network. Thus, the findings of this study advance our understanding of the central role played by bHLH142 in the regulatory network leading to pollen development in rice and how overexpression of its expression affects pollen development. Exploitation of this novel functionality of bHLH142 may confer a big advantage to hybrid seed production.

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“…() and Ranjan et al . (). Tissue blocks of the appropriate size and orientation were sectioned using a rotary microtome (Leica Biosystems, USA) to obtain strips of 8‐ to 10‐μm‐thick sections, which were flattened by floating for 1–2 min at 50–55°C.…”

Section: Methodsmentioning

confidence: 97%

“…() and Ranjan et al . (). The dehydrated samples were further processed by critical‐point drying and examined by SEM (Evo LS25; Zeiss).…”

Section: Methodsmentioning

confidence: 97%

Transcriptional signatures modulating shoot apical meristem morphometric and plant architectural traits enhance yield and productivity in chickpea

et al. 2019

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Summary Plant height (PH) and plant width (PW), two of the major plant architectural traits determining the yield and productivity of a crop, are defined by diverse morphometric characteristics of the shoot apical meristem (SAM). The identification of potential molecular tags from a single gene that simultaneously modulates these plant/SAM architectural traits is therefore prerequisite to achieve enhanced yield and productivity in crop plants, including chickpea. Large‐scale multienvironment phenotyping of the association panel and mapping population have ascertained the efficacy of three vital SAM morphometric trait parameters, SAM width, SAM height and SAM area, as key indicators to unravel the genetic basis of the wide PW and PH trait variations observed in desi chickpea. This study integrated a genome‐wide association study (GWAS); quantitative trait locus (QTL)/fine‐mapping and map‐based cloning with molecular haplotyping; transcript profiling; and protein‐DNA interaction assays for the dissection of plant architectural traits in chickpea. These exertions delineated natural alleles and superior haplotypes from a CabHLH121 transcription factor (TF) gene within the major QTL governing PW, PH and SAM morphometric traits. A genome‐wide protein‐DNA interaction assay assured the direct binding of a known stem cell master regulator, CaWUS, to the WOX‐homeodomain TF binding sites of a CabHLH121 gene and its constituted haplotypes. The differential expression of CaWUS and transcriptional regulation of its target CabHLH121 gene/haplotypes were apparent, suggesting their collective role in altering SAM morphometric characteristics and plant architectural traits in the contrasting near isogenic lines (NILs). The NILs introgressed with a superior haplotype of a CabHLH121 exhibited optimal PW and desirable PH as well as enhanced yield and productivity without compromising any component of agronomic performance. These molecular signatures of the CabHLH121 TF gene have the potential to regulate both PW and PH traits through the modulation of proliferation, differentiation and maintenance of the meristematic stem cell population in the SAM; therefore, these signatures will be useful in the translational genomic study of chickpea genetic enhancement. The restructured cultivars with desirable PH (semidwarf) and PW will ensure maximal planting density in a specified cultivable field area, thereby enhancing the overall yield and productivity of chickpea. This can essentially facilitate the achievement of better remunerative outputs by farmers with rational land use, therefore ensuring global food security in the present scenario of an increasing population density and shrinking per capita land area.

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bHLH142 regulates various metabolic pathway-related genes to affect pollen development and anther dehiscence in rice

Cited by 39 publications

References 57 publications

Ornithine δ‐aminotransferase is critical for floret development and seed setting through mediating nitrogen reutilization in rice

Ornithine δ‐aminotransferase is critical for floret development and seed setting through mediating nitrogen reutilization in rice

Tightly Controlled Expression of bHLH142 Is Essential for Timely Tapetal Programmed Cell Death and Pollen Development in Rice

Transcriptional signatures modulating shoot apical meristem morphometric and plant architectural traits enhance yield and productivity in chickpea

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