bHLH genes polymorphisms and their association with growth traits in the Pacific oyster Crassostrea gigas

Chen, Na; Li, Li; Li, Chenghua; Zhang, Lin; Meng, Jie; Liu, Sheng; Song, Kai; Bao, Yongbo

doi:10.1007/s00343-019-9070-4

Cited by 2 publications

(1 citation statement)

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“…Numerous studies have shown that bHLH transcription factors play essential roles in the physiological process of plants, such as plant growth and development [ 11 , 12 , 13 , 14 ], abiotic stress response [ 15 , 16 , 17 , 18 ], and anthocyanin biosynthesis [ 19 , 20 , 21 ]. Lc was the first bHLH protein identified in plants, and it was shown to regulate anthocyanin biosynthesis in maize [ 22 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-Wide Identification and Analysis of bHLH Transcription Factors Related to Anthocyanin Biosynthesis in Cymbidium ensifolium

Wang

Zuo

et al. 2023

IJMS

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The basic helix-loop-helix (bHLH) transcription factors are widely distributed across eukaryotic kingdoms and participate in various physiological processes. To date, the bHLH family has been identified and functionally analyzed in many plants. However, systematic identification of bHLH transcription factors has yet to be reported in orchids. Here, 94 bHLH transcription factors were identified from the Cymbidium ensifolium genome and divided into 18 subfamilies. Most CebHLHs contain numerous cis-acting elements associated with abiotic stress responses and phytohormone responses. A total of 19 pairs of duplicated genes were found in the CebHLHs, of which 13 pairs were segmentally duplicated genes and six pairs were tandemly duplicated genes. Expression pattern analysis based on transcriptome data revealed that 84 CebHLHs were differentially expressed in four different color sepals, especially CebHLH13 and CebHLH75 of the S7 subfamily. The expression profiles of CebHLH13 and CebHLH75 in sepals, which are considered potential genes regulating anthocyanin biosynthesis, were confirmed through the qRT-PCR technique. Furthermore, subcellular localization results showed that CebHLH13 and CebHLH75 were located in the nucleus. This research lays a foundation for further exploration of the mechanism of CebHLHs in flower color formation.

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