BHK 21 C13 cells for Aujeszky’s disease virus production using the multiple harvest process

Srček, Višnja Gaurina; Čajavec, Stanislav; Sladić, Dušan; Kniewald, Zlatko

doi:10.1007/s10616-004-2551-z

Cited by 7 publications

(2 citation statements)

References 13 publications

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“…Glutamine metabolism can provide 30-65% of the energy required for mammalian cell growth (Reitzer et al 1979;Zielke et al 1984). Most of mammalian cell lines die immediately once the glucose is depleted (Mercille and Massie 1994;Gaurina Srček et al 2004). However, if glutamine is depleted, some cell lines can not survive but others can adapt to conditions lacking of glutamine (McDermott and Butler 1993).…”

Section: Introductionmentioning

confidence: 99%

Growth characteristics of channel catfish ovary cells—influence of glucose and glutamine

et al. 2008

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The growth characteristics and influence of glucose and glutamine on the growth and maintenance of channel catfish ovary (CCO) cells were investigated. Besides glutamine, amino acids threonine, arginine, methionine and serine were found to be essential for CCO cell growth. In the glucose-free medium, glutamine is utilized as energy source and no cell growth limitation was observed. However, the lack of glutamine in culture medium did not stimulate CCO cells to efficient glucose consumption. When both glucose and glutamine were deficient, cell growth was also observed suggesting no rigorous nutritional requirements. Obtained results are useful for further understanding of culture processes using CCO cells.

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Section: Introductionmentioning

confidence: 99%

Growth characteristics of channel catfish ovary cells—influence of glucose and glutamine

et al. 2008

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“…BHK-21 cells are widely used in biotechnological production of therapeutics and veterinary vaccines particularly rabies (Perrin et al 1995;Kallel et al 2002), foot and mouth disease (Radlett et al 1985), and Aujeszky's disease (Gaurina Srček et al 2005). Proliferative effect of C-BE on primary cell cultures was also confirmed on BHK-21 [C-13] (data not showed) and further experiments were done with P-BE.…”

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confidence: 97%

Effect of porcine brain growth factor on primary cell cultures and BHK-21 [C-13] cell line

Srček

Radošević

Kniewald

et al. 2008

In Vitro Cell.Dev.Biol.-Animal

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Growth factors from neural tissues have been described as potent mitogens for a wide variety of mesoderm- and ectoderm-derived cells in vitro. We used porcine brain extract for in vitro testing of proliferation properties on primary ovarian cells, uterine cells, and cardiomyocytes in culture as well as for BHK-21 [C-13] cell line. The addition of this extract accelerates proliferation in all examined cultures. It also lowers serum requirement and shortens the cultivation period for BHK-21 [C-13] cells. Fibroblast growth factors from brain of different species, but not porcine, are already characterized and their proliferative effect proved. Therefore, we purified, determined, and confirmed the presence of basic fibroblast growth factor in porcine brain extract by Western blot analysis and showed its biological activity on BHK-21 [C-13] cells.

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Aujeszky’s disease virus production in disposable bioreactor

et al. 2006

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A novel, disposable-bag bioreactor system that uses wave action for mixing and transferring oxygen was evaluated for BHK 21 C13 cell line growth and Aujeszky's disease virus (ADV) production. Growth kinetics of BHK 21 C13 cells in the wave bioreactor during 3-day period were determined. At the end of the 3-day culture period and cell density of 1.82x10(6) cells ml-1, the reactor was inoculated with 9 ml of gE- Bartha K-61 strain ADV suspension (10(5.9) TCID50) with multiplicity of infection (MOI) of 0.01. After a 144 h incubation period, 400 ml of ADV harvest was obtained with titre of 10(7.0) TCID 50 ml-1, which corresponds to 40,000 doses of vaccine against AD. In conclusion, the results obtained with the wave bioreactor using BHK 21 C13 cells showed that this system can be considered as suitable for ADV or BHK 21 C13 cell biomass production.

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BHK 21 C13 cells for Aujeszky’s disease virus production using the multiple harvest process

Cited by 7 publications

References 13 publications

Growth characteristics of channel catfish ovary cells—influence of glucose and glutamine

Growth characteristics of channel catfish ovary cells—influence of glucose and glutamine

Effect of porcine brain growth factor on primary cell cultures and BHK-21 [C-13] cell line

Aujeszky’s disease virus production in disposable bioreactor

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