“…These data correlate well with the results of the genetic analysis of the mutants shown above (Table III). Those proteins that were not (mutant H101L; lane 21) or only weakly (mutants C76R, D100(G,N,V), E153V; lanes 7,15,17,19,25) phosphorylated by EII Bgl , were also completely independent of negative control, whereas those proteins that were more efficiently phosphorylated (mutants I80T, Q91R, S97P, F104V, and H160R; lanes 9,11,13,23,27), were still subject to residual repression by EII Bgl (Table III). This correlation suggests that the level of escape from repression seen for the individual mutant proteins may indeed be caused by a corresponding less efficient EII Bgl -catalyzed phosphorylation.…”