bFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway

Chakrabarti, Srijita; Mazumder, Bhaskar; Rajkonwar, Jadab; Pathak, Manash Pratim; Patowary, Pompy; Chattopadhyay, Pronobesh

doi:10.1038/s41598-021-82888-9

Cited by 34 publications

(23 citation statements)

References 53 publications

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“…CSL optical collagen densities were substantially higher than those of the CA/SA group (p < 0.05, n = 3). Besides, in this study showed that the collagen in CSL group arranged regularly, and the new epidermis was much closer in appearance to that of normal skin, which indicated that CSL can accelerate re-epithelization and wound repairing [57]. These results were consistent with those of the in vitro experiments, indicating that LAP could help to enhance wound healing through promoting cell proliferation, migration, and collagen secretion.…”

Section: Histological Evaluationsupporting

confidence: 87%

An injectable cellulose acetate/sodium alginate hydrogels-loaded laponite microsphere as a potential wound healing in nursing care in perioperative period

Wang¹,

Yang

Zhao

2022

Mater. Res. Express

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The aim of this study was developed by a simple method including solution mixing of cellulose acetate/sodium alginate containing laponite (CA/SA-LAP) hydrogel composites with flexible mechanical, physical, and biological properties for wound healing application. The surface properties of the as-prepared CA/SA hydrogel and CA/SA-LAP hydrogel composites were characterized by Fourier transform infrared spectroscopy (FT-IR) and X-Ray Diffraction (XRD). The influence of LAP content (0.1, 0.2, 0.5 wt%), the swelling ratio (171.0 ± 7.0, 143.0 ± 4.0, and 96.6 ± 2.5), degradation rate (65.7 ± 4.0%, 49.0 ± 5.5%, and 19.4 ± 1.4%), mechanical properties elongation at break from 68.3 ± 6.0% to 148.3 ± 5.6%, and the morphology of the CA/SA-LAP was investigated. By increasing the LAP concentration with an average pore size diameter decreased from 300  μm to 225  μm, 150  μm, and 75 μm. The as-prepared CA/SA-LAP dressing has designated good antimicrobial activity against Staphylococcus aureus and Escherichia coli bacteria at 95.9 ± 4.0% and 98.4 ± 1.5% for 24 h and 92.3 ± 4.5% and 96.4 ± 3.5% for 48 h. Furthermore, CA/SA-LAP revealed admirable biocompatibility against human osteosarcoma cell line MG-63. Noticeably, the MTT assay demonstrated that fibroblast proliferation significantly enhanced on 0.5 wt.% LAP in CA/SA-LAP compared to CS/SA hydrogel at 92.6 ± 4.2% and 96.4 ± 3.5% for 24 and 48 h. Systematic in vivo research of the CA/SA-LAP was conducted in the rat bone defect model. The in vivo results proved that the CA/SA-loaded LAP significantly promoted bone healing in rat defects, compared to the CA/SA hydrogels. These results demonstrated the great potential of CA/SA-loaded LAP in wound healing material in nursing care application.

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Section: Histological Evaluationsupporting

confidence: 87%

An injectable cellulose acetate/sodium alginate hydrogels-loaded laponite microsphere as a potential wound healing in nursing care in perioperative period

Wang¹,

Yang

Zhao

2022

Mater. Res. Express

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“…Also, collagen hydrogels provided a prolonged antibacterial activity when they involved simvastatin, and findings revealed that these materials could stimulate macrophages which is useful in wound healing processes [26]. e MTT assay of Chakrabarti et al study showed that bFGF and collagen matrix hydrogel had minimal toxicity on the L929 mouse fibroblast cell line, did not inhibit cell proliferation, and could be further investigated in burn wounds [27]. In the present study, the antibacterial evaluations showed that the number reduction of E. coli was more than S. aureus.…”

Section: Discussionmentioning

confidence: 99%

The Wound Healing Effect of Nanoclay, Collagen, and Tadalafil in Diabetic Rats: An In Vivo Study

Ordeghan

Khayatan

Saki

et al. 2022

Advances in Materials Science and Engineering

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The diabetic wound is the most challenging one to manage, which is associated with microvascular and macrovascular dysfunction, and novel strategies such as using hydrogels demonstrate their promising prospect in treatment and management approaches of the diabetic wound. This study aimed to investigate the effect of collagen/nanoclay/tadalafil hydrogel on wound healing in diabetic rats under HIIT exercise. Hydrogel was synthesized, and then biocompatibility and antibacterial tests were performed. The therapeutic effect of collagen/nanoclay/tadalafil hydrogel was assessed after induction of diabetes in the rat model, and wound healing was evaluated with macroscopic and microscopic tests. The result of the MTT test showed no significant cytotoxicity of collagen/nanoclay/tadalafil hydrogel. Furthermore, the inhibitory effect of hydrogel was detected on E. coli and S. aureus. The macroscopic results demonstrated that the wound contraction was considerable in the hydrogel/HIIT exercise and hydrogel groups compared with the HIIT exercise and control groups during 21 days. The microscopic results showed that the presence of fibroblasts, the amount of collagen, the epidermis density, and the formation of hair follicles were increased in the hydrogel/HIIT exercise group compared with other groups in the diabetic rate model. It can be concluded that collagen/nanoclay/tadalafil hydrogel with HIIT exercise could accelerate diabetic wound healing and can be an appropriate candidate for skin regeneration in medical applications.

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“…bFGF stimulated migration of fibroblasts and synthesis of collagen, as well as promoted angiogenesis. It also promoted wound healing by pathways for nerve growth factor (NGF), tropomyosin-receptor kinase A (TrkA), p-TrkA, extracellular regulated kinase 1 and 2 (ERK1/2), p-ERK1/2, NF-kβ, and p-NF-kβ [ 55 ].…”

Section: Promotion Of Wound Healingmentioning

confidence: 99%

Hydrogels in Burn Wound Management—A Review

Surowiecka¹,

Strużyna

Winiarska³

et al. 2022

Gels

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Inert hydrogels are of a great importance in burn first aid. Hydrogel dressings may be an alternative to cooling burn wounds with streaming water, especially in cases of mass casualty events, lack of clean water, hypothermia, or large extent of burns. Hydrogels that contain mostly water evacuate the heat cumulating in the skin by evaporation. They not only cool the burn wound, but also reduce pain and protect the wound area from contamination and further injuries. Hydrogels are ideally used during the first hours after injury, but as they do not have antimicrobial properties per se, they might not prevent wound infection. The hydrogel matrix enables incorporating active substances into the dressing. The active forms may contain ammonium salts, nanocrystal silver, zinc, growth factor, cytokines, or cells, as well as natural agents, such as honey or herbs. Active dressings may have antimicrobial activity or stimulate wound healing. Numerous experiments on animal models proved their safety and efficiency. Hydrogels are a new dressing type that are still in development.

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bFGF and collagen matrix hydrogel attenuates burn wound inflammation through activation of ERK and TRK pathway

Cited by 34 publications

References 53 publications

An injectable cellulose acetate/sodium alginate hydrogels-loaded laponite microsphere as a potential wound healing in nursing care in perioperative period

An injectable cellulose acetate/sodium alginate hydrogels-loaded laponite microsphere as a potential wound healing in nursing care in perioperative period

The Wound Healing Effect of Nanoclay, Collagen, and Tadalafil in Diabetic Rats: An In Vivo Study

Hydrogels in Burn Wound Management—A Review

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