BFA‐induced compartments from the Golgi apparatus and <i>trans</i>‐Golgi network/early endosome are distinct in plant cells

Lam, Sheung Kwan; Cai, Yi; Tse, Yu Chung; Wang, Juan; Law, Angus Ho Yin; Pimpl, Peter; Chan, Ho Yin Edwin; Xia, Jun; Jiang, Liwen

doi:10.1111/j.1365-313x.2009.04007.x

Cited by 108 publications

(109 citation statements)

References 60 publications

(113 reference statements)

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“…Furthermore, during this early stage, cell plate formation is prevented when ER-toGolgi transport is inhibited by low BFA concentration (Ritzenthaler et al, 2002;Lam et al, 2009;Langhans et al, 2011) or when CHX is administered prior to DNA condensation to interfere with de novo protein synthesis. When CHX was applied after DNA segregation, the cell plate was initiated unhindered.…”

Section: Discussionmentioning

confidence: 99%

“…5). In tobacco cells used at low concentration (50 mM), the ARF-GEF inhibitor brefeldin A (BFA) blocks secretory vesicle trafficking (Grebe et al, 2003;Dhonukshe et al, 2006;Tse et al, 2006;Reichardt et al, 2007;Lam et al, 2009) and induces the formation of a hybrid endoplasmic reticulum (ER)-Golgi compartment as well as a BFA compartment consisting of a mixture of the TGN vesicles and endosomal vesicles. However, it does not affect the late endosomal compartment labeled with YFP-ARA7 (Ueda et al, 2004;Dhonukshe et al, 2006; Fig.…”

Section: Endocytic Pathways Are Involved In the Expansion Of The Cellmentioning

confidence: 99%

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Vesicle Dynamics during Plant Cell Cytokinesis Reveals Distinct Developmental Phases

et al. 2017

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Cell division in plant cells requires the deposition of a new cell wall between the two daughter cells. The assembly of this plate requires the coordinated movement of cargo vesicles whose size is below the diffraction-limited resolution of the optical microscope. We combined high spatial and temporal resolution confocal laser scanning microscopy with advanced imageprocessing tools and fluorescence fluctuation methods and distinguished three distinct phases during cell plate expansion in tobacco (Nicotiana tabacum) 'Bright Yellow-2' cells: massive delivery of preexisting vesicles to a disk-shaped region at the equatorial plane precedes a primary rapid expansion phase followed by a secondary, slow expansion phase during which the extremity of the circular plate seeks contact with the mother wall and brings about the separation of the two portions of cytoplasm. Different effects of pharmacological inhibition emphasize the distinct nature of the assembly and expansion mechanisms characterizing these phases.

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Section: Discussionmentioning

confidence: 99%

Section: Endocytic Pathways Are Involved In the Expansion Of The Cellmentioning

confidence: 99%

Vesicle Dynamics during Plant Cell Cytokinesis Reveals Distinct Developmental Phases

et al. 2017

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“…In Arabidopsis, the trafficking inhibitor BFA targets the Arf-GEF GNOM and induces the formation of BFA compartments (Geldner et al, 2001), most likely by homotypic fusion of the TGN (which is also an early endosome) and heterotypic fusion between the TGN and other endosomes (Dettmer et al, 2006;Lam et al, 2009;Viotti et al, 2010). Therefore, SNAREs at the TGN mayfunction in these fusion events upon BFA treatment.…”

Section: Involvement Of Tno1 In Vesicle Fusionmentioning

confidence: 99%

TNO1 Is Involved in Salt Tolerance and Vacuolar Trafficking in Arabidopsis

Kim

Bassham

2011

Plant Physiology

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The Arabidopsis (Arabidopsis thaliana) soluble N-ethylmaleimide-sensitive factor attachment protein receptor SYP41 is involved in vesicle fusion at the trans-Golgi network (TGN) and interacts with AtVPS45, SYP61, and VTI12. These proteins are involved in diverse cellular processes, including vacuole biogenesis and stress tolerance. A previously uncharacterized protein, named TNO1 (for TGN-localized SYP41-interacting protein), was identified by coimmunoprecipitation as a SYP41-interacting protein.TNO1 was found to localize to the TGN by immunofluorescence microscopy. A tno1 mutant showed increased sensitivity to high concentrations of NaCl, KCl, and LiCl and also to mannitol-induced osmotic stress. Localization of SYP61, which is involved in the salt stress response, was disrupted in the tno1 mutant. Vacuolar proteins were partially secreted to the apoplast in the tno1 mutant, suggesting that TNO1 is required for efficient protein trafficking to the vacuole. The tno1 mutant had delayed formation of the brefeldin A (BFA) compartment in cotyledons upon application of BFA, suggesting less efficient membrane fusion processes in the mutant. Unlike most TGN proteins, TNO1 does not relocate to the BFA compartment upon BFA treatment. These data demonstrate that TNO1 is involved in vacuolar trafficking and salt tolerance, potentially via roles in vesicle fusion and in maintaining TGN structure or identity.

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“…BFA treatment resulted in the accumulation of most PAT10-positive signals into a circle surrounding the FM4-64-labeled BFA cores (Fig. 2, B and D), indicative of its Golgi identity (Lam et al, 2009). …”

Section: Targeting Of Pat10 To the Tonoplast Is Independent Of Rab5-mmentioning

confidence: 99%

Adaptor Protein-3-Dependent Vacuolar Trafficking Involves a Subpopulation of COPII and HOPS Tethering Proteins

Feng

Song

et al. 2017

Plant Physiol.

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ORCID IDs: 0000-0001-6345-6855 (J.-G.W.); 0000-0002-3501-5857 (Y.Z.).Plant vacuoles are versatile organelles critical for plant growth and responses to environment. Vacuolar proteins are transported from the endoplasmic reticulum via multiple routes in plants. Two classic routes bear great similarity to other phyla with major regulators known, such as COPII and Rab5 GTPases. By contrast, vacuolar trafficking mediated by adaptor protein-3 (AP-3) or that independent of the Golgi has few recognized cargos and none of the regulators. In search of novel regulators for vacuolar trafficking routes and by using a fluorescence-based forward genetic screen, we demonstrated that the multispan transmembrane protein, Arabidopsis (Arabidopsis thaliana) PROTEIN S-ACYL TRANSFERASE10 (PAT10), is an AP-3-mediated vacuolar cargo. We show that the tonoplast targeting of PAT10 is mediated by the AP-3 complex but independent of the Rab5-mediated post-Golgi trafficking route. We also report that AP-3-mediated vacuolar trafficking involves a subpopulation of COPII and requires the vacuolar tethering complex HOPS. In addition, we have identified two novel mutant alleles of AP-3d, whose point mutations interfered with the formation of the AP-3 complex as well as its membrane targeting. The results presented here shed new light on the vacuolar trafficking route mediated by AP-3 in plant cells.

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BFA‐induced compartments from the Golgi apparatus and trans‐Golgi network/early endosome are distinct in plant cells

Cited by 108 publications

References 60 publications

Vesicle Dynamics during Plant Cell Cytokinesis Reveals Distinct Developmental Phases

Vesicle Dynamics during Plant Cell Cytokinesis Reveals Distinct Developmental Phases

TNO1 Is Involved in Salt Tolerance and Vacuolar Trafficking in Arabidopsis

Adaptor Protein-3-Dependent Vacuolar Trafficking Involves a Subpopulation of COPII and HOPS Tethering Proteins

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