Beyond plant defense: insights on the potential of salicylic and methylsalicylic acid to contain growth of the phytopathogen Botrytis cinerea

Abstract

Using Botrytis cinerea we confirmed in the present work several previous studies showing that salicylic acid, a main plant hormone, inhibits fungal growth in vitro. Such an inhibitory effect was also observed for the two salicylic acid derivatives, methylsalicylic and acetylsalicylic acid. In marked contrast, 5-sulfosalicylic acid was totally inactive. Comparative proteomics from treated vs. control mycelia showed that both the intracellular and extracellular proteomes were affected in the presence of salicylic acid or methylsalicylic acid. These data suggest several mechanisms that could potentially account for the observed fungal growth inhibition, notably pH regulation, metal homeostasis, mitochondrial respiration, ROS accumulation and cell wall remodeling. The present observations support a role played by the phytohormone SA and derivatives in directly containing the pathogen. Data are available via ProteomeXchange with identifier PXD002873.

Salicylic acid as an effective elicitor for improved taxol production in endophytic fungus Pestalotiopsis microspora

“…The protein bands obtained from SA treated mycelia of P . microspora were similar to 2.5 mM SA treated protein spots previously reported in the fungus Botrytis cinerea [61] with clear matches of protein bands at 14, 19, 35, 44, and 56 KDa obtained in this study [61]. Further, we observed that SA induced protein expression related to the lipid metabolism and tricarboxylic acid (TCA) pathway (Fig 8).…”

“…Moreover, BLAST search against B . cinerea peptide sequence confirmed the presence of biosynthetic enzymes involved in the function of virulence factor (19-kDa protein), immunity and defense (19-kDa protein), TCA pathway (34.6-kDa protein), disease/defence (34.5-kDa protein), lipid metabolism (35.2-kDa and 56.1-kDa proteins), carbohydrate metabolism (44.1 kDa), nucleic acid metabolism (45.7-kDa protein), protein metabolism and modification (55.2-kDa protein) [61].…”

The Multiple Facets of Plant–Fungal Interactions Revealed Through Plant and Fungal Secretomics

“…Together, these findings show that a central regulator of the plant immune system, largely uncharacterized in roots, directly influences the root microbiota composition (Lebeis et al, 2015). Consistent with this, exogenous SA and derivatives proved able to inhibit the growth of B. cinerea in vitro (Dieryckx et al, 2015). Similarly, several studies showed that ABA exhibits antifungal activity in plants (Khedr et al, 2018).…”

Proteomic Analysis of Kiwifruit in Response to the Postharvest Pathogen, Botrytis cinerea

“…Proteomics has emerged as a powerful tool for understanding the molecular mechanism of plant-pathogen interactions (Imam et al, 2017 ). Using proteomics, the response of B. cinerea to plant-based elicitors and hormones (Dieryckx et al, 2015 ; Liñeiro et al, 2016 ), and the in vitro secretome of B. cinerea related to pathogenesis (González-Fernández et al, 2015 ) have been characterized. In general, proteomic analyses of plant hosts in response to fungal pathogens have been widely reported in recent years.…”