Beyond Dried Blood Spots—Application of Dried Matrix Spots

Needham, Shane

doi:10.1002/9781118890837.ch21

Cited by 2 publications

(2 citation statements)

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“…However, reports describe significant differences between punctures performed in the center and in the peripheral area of the paper . One of the main described limitations of dried spot applied to colorless matrices is the possibility of performing a partial puncture of the material due to the difficulty in identifying the precise place where the application was made . However, data obtained after evaluation of spot homogeneity corroborate good homogeneity, since the results obtained from the puncture done in peripheral and central regions of the paper in triplicate showed satisfactory accuracy, varying less than 15% of the nominal concentration.…”

Section: Resultsmentioning

confidence: 99%

“…Filter paper can retain major components of the matrix, allowing the easy extraction of the target analyte . In recent years, alternative matrices have also been prominent, with an increasing number of publications using other matrices rather than blood as dried spots and showing promising results in several different fields of analysis . Dried spot sampling technique involves the collection of a small volume of biological fluid directly onto filter paper, followed by drying, puncture of the spot deposited on the paper, an extraction procedure and analysis.…”

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confidence: 99%

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Development and Validation of a Method Using Dried Oral Fluid Spot to Determine Drugs of Abuse

Jacques

Santos

Limberger

2019

Journal of Forensic Sciences

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A liquid chromatography–mass spectrometry method using dried oral fluid spots was developed and validated for the simultaneous quantification of cocaine, benzoylecgonine, cocaethylene, amphetamine, and 3,4‐methylenedioxymethamphetamine. The oral fluid was applied to a Whatman 903 grade paper and submitted to a drying time of 2.5 h. The extraction procedure was optimized by chemometric approach using simplex centroid design. Spots were extracted with a mixture of acetonitrile, buffer, and methanol. Calibration curves covered a linear concentration range of 40–500 ng/mL. Validation parameters of linearity, precision, accuracy, selectivity, carryover, matrix effects, and stability were evaluated and showed satisfactory results. Spot homogeneity was also satisfactory, with less than 15% of deviation from nominal concentration. Spot volume did not influence accuracy when less than 100 μL of the sample was applied to the spot. The validation of the proposed method suggests a potential application in different scenarios in toxicology.

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Section: Resultsmentioning

confidence: 99%

mentioning

confidence: 99%

Development and Validation of a Method Using Dried Oral Fluid Spot to Determine Drugs of Abuse

Jacques

Santos

Limberger

2019

Journal of Forensic Sciences

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The Development of Patient-Centric Sampling as an Enabling Technology for Clinical Trials

Bateman¹

2020

Bioanalysis

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Accessing patient samples using a whenever/wherever paradigm is needed to enable a better understanding of human biology and disease. The technology for convenient self-collection of blood samples by patients at home is quickly becoming available. The potential benefits of patient-centric sampling far outweigh the short-term challenges associated with implementation of this disruptive approach. This is especially true given we are amid a global pandemic and enabling patients to sample at home would help not only clinical trials, but healthcare in general. This perspective article aims to convince the reader that patient-centric sampling is a reality and that we are on the cusp of an information revolution in clinical trials that will be enabled by patient-centric (e.g., at home) sampling.

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Beyond Dried Blood Spots—Application of Dried Matrix Spots

Cited by 2 publications

References 25 publications

Development and Validation of a Method Using Dried Oral Fluid Spot to Determine Drugs of Abuse

Development and Validation of a Method Using Dried Oral Fluid Spot to Determine Drugs of Abuse

The Development of Patient-Centric Sampling as an Enabling Technology for Clinical Trials

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