Bex1 is essential for ciliogenesis and harbours biomolecular condensate-forming capacity

Hibino, Emi; Ichiyama, Yusuke; Tsukamura, Atsushi; Senju, Yosuke; Morimune, Takao; Ohji, Masahito; Maruo, Yoshihiro; Nakagawa, Masaki; Mori, Masaki

doi:10.1186/s12915-022-01246-x

Cited by 6 publications

(2 citation statements)

References 70 publications

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“…Brain-expressed X-linked 1 ( BEX1 ) belongs to the BEX gene family, which consists of BEX1 , BEX2 , BEX3 , BEX4 , Tceal1 , Tceal3 , Tceal5 , Tceal6 , Tceal7 , Tceal8 , and Tceal9 [ 16 ]. In agreement with our findings, AronowBJ et al found that BEX1 showed upregulated expression levels in the hearts of mice with heart failure [ 17 ].…”

Section: Discussionmentioning

confidence: 99%

Identification of Signature Genes of Dilated Cardiomyopathy Using Integrated Bioinformatics Analysis

Wang

Liang

et al. 2023

IJMS

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Dilated cardiomyopathy (DCM) is characterized by left ventricular or biventricular enlargement with systolic dysfunction. To date, the underlying molecular mechanisms of dilated cardiomyopathy pathogenesis have not been fully elucidated, although some insights have been presented. In this study, we combined public database resources and a doxorubicin-induced DCM mouse model to explore the significant genes of DCM in full depth. We first retrieved six DCM-related microarray datasets from the GEO database using several keywords. Then we used the “LIMMA” (linear model for microarray data) R package to filter each microarray for differentially expressed genes (DEGs). Robust rank aggregation (RRA), an extremely robust rank aggregation method based on sequential statistics, was then used to integrate the results of the six microarray datasets to filter out the reliable differential genes. To further improve the reliability of our results, we established a doxorubicin-induced DCM model in C57BL/6N mice, using the “DESeq2” software package to identify DEGs in the sequencing data. We cross-validated the results of RRA analysis with those of animal experiments by taking intersections and identified three key differential genes (including BEX1, RGCC and VSIG4) associated with DCM as well as many important biological processes (extracellular matrix organisation, extracellular structural organisation, sulphur compound binding, and extracellular matrix structural components) and a signalling pathway (HIF-1 signalling pathway). In addition, we confirmed the significant effect of these three genes in DCM using binary logistic regression analysis. These findings will help us to better understand the pathogenesis of DCM and may be key targets for future clinical management.

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Section: Discussionmentioning

confidence: 99%

Identification of Signature Genes of Dilated Cardiomyopathy Using Integrated Bioinformatics Analysis

Wang

Liang

et al. 2023

IJMS

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“…Differential expression analysis across GE-SCJ epithelial cell clusters unraveled the gene expression signature associated with embryonic precursor epithelial cells (Sox11, Igf2, H19, Cldn6, Vcan, and Bex1) [18][19][20][21][22][23][24] committing to either the squamous (Trp63, Col17a1, Krt5, Krt15, Krt13, Lgals7) or columnar (Muc5b, Furin, Pgc, Muc6, Agr2) epithelial lineages (Fig. 1f, g, and Supplementary Data 2).…”

Section: Single-cell Map Of Epithelial Lineage Development At the Ge-scjmentioning

confidence: 99%

Decoding spatiotemporal transcriptional dynamics and epithelial fibroblast crosstalk during gastroesophageal junction development through single cell analysis

Kumar,

Prakash,

Wentland

et al. 2024

Nat Commun

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The gastroesophageal squamocolumnar junction (GE-SCJ) is a critical tissue interface between the esophagus and stomach, with significant relevance in the pathophysiology of gastrointestinal diseases. Despite this, the molecular mechanisms underlying GE-SCJ development remain unclear. Using single-cell transcriptomics, organoids, and spatial analysis, we examine the cellular heterogeneity and spatiotemporal dynamics of GE-SCJ development from embryonic to adult mice. We identify distinct transcriptional states and signaling pathways in the epithelial and mesenchymal compartments of the esophagus and stomach during development. Fibroblast-epithelial interactions are mediated by various signaling pathways, including WNT, BMP, TGF-β, FGF, EGF, and PDGF. Our results suggest that fibroblasts predominantly send FGF and TGF-β signals to the epithelia, while epithelial cells mainly send PDGF and EGF signals to fibroblasts. We observe differences in the ligands and receptors involved in cell-cell communication between the esophagus and stomach. Our findings provide insights into the molecular mechanisms underlying GE-SCJ development and fibroblast-epithelial crosstalk involved, paving the way to elucidate mechanisms during adaptive metaplasia development and carcinogenesis.

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Known types of membrane-less organelles and biomolecular condensates

Darling¹,

Uversky²

2023

Droplets of Life

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Bex1 is essential for ciliogenesis and harbours biomolecular condensate-forming capacity

Cited by 6 publications

References 70 publications

Identification of Signature Genes of Dilated Cardiomyopathy Using Integrated Bioinformatics Analysis

Identification of Signature Genes of Dilated Cardiomyopathy Using Integrated Bioinformatics Analysis

Decoding spatiotemporal transcriptional dynamics and epithelial fibroblast crosstalk during gastroesophageal junction development through single cell analysis

Known types of membrane-less organelles and biomolecular condensates

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