Betulinic acid attenuates renal oxidative stress and inflammation in experimental model of murine polymicrobial sepsis

Lingaraju, Madhu Cholenahalli; Pathak, N.N.; Begum, Jubeda; Balaganur, Venkanna; Ramachandra, Harish Darasaguppe; Bhat, Rafia Ahmad; Ram, Mahendra; Singh, Vishal; Kandasamy, Kannan; Kumar, Dhirendra; Kumar, Dinesh; Tandan, Surendra K.

doi:10.1016/j.ejps.2015.01.001

Cited by 40 publications

(26 citation statements)

References 71 publications

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“…The samples were then read at 540 nm against a standard sodium nitrite curve. The amount of nitrite in the samples was calculated from a sodium nitrite standard curve (Lingaraju et al, ).…”

Section: Methodsmentioning

confidence: 99%

Diarylheptanoids Rich Fraction ofAlnus nepalensisAttenuates Malaria Pathogenesis:In-vitroandIn-vivoStudy

et al. 2016

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Diarylheptanoids from Alnus nepalensis leaves have been reported for promising activity against filariasis, a mosquito-borne disease, and this has prompted us to investigate its anti-malarial and safety profile using in-vitro and in-vivo bioassays. A. nepalensis leaf extracts were tested in-vitro against chloroquine-sensitive Plasmodium falciparum NF54 by measuring the parasite specific lactate dehydrogenase activity. Among all, the chloroform extract (ANC) has shown promising anti-plasmodial activity (IC50 8.06 ± 0.26 µg/mL). HPLC analysis of ANC showed the presence of diarylheptanoids. Efficacy and safety of ANC were further validated in in-vivo system using Plasmodium berghei-induced malaria model and acute oral toxicity in mice. Malaria was induced by intra-peritoneal injection of P. berghei infected red blood cells to the female Balb/c mice. ANC was administered orally at doses of 100 and 300 mg/kg/day following Peter's 4 day suppression test. Oral administration of ANC showed significant reduction of parasitaemia and increase in mean survival time. It also attributed to inhibition of the parasite induced pro-inflammatory cytokines as well as afford to significant increase in the blood glucose and haemoglobin level when compared with vehicle-treated infected mice. In-vivo safety evaluation study revealed that ANC is non-toxic at higher concentration. Copyright © 2016 John Wiley & Sons, Ltd.

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“…The samples were then read at 540 nm against a standard sodium nitrite curve. The amount of nitrite in the samples was calculated from a sodium nitrite standard curve (Lingaraju et al, ).…”

Section: Methodsmentioning

confidence: 99%

Diarylheptanoids Rich Fraction ofAlnus nepalensisAttenuates Malaria Pathogenesis:In-vitroandIn-vivoStudy

et al. 2016

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“… 2015 ; Lingaraju et al. 2015 ). Therefore, this study assessed the healing effect of different extracts from the fruit of D. indica standardized to betulinic acid on ultraviolet (UV) radiation-induced psoriasis-like wounds in rats.…”

Section: Introductionmentioning

confidence: 99%

Healing effect of Dillenia indica fruit extracts standardized to betulinic acid on ultraviolet radiation-induced psoriasis-like wounds in rats

Kviecinski

David

Fernandes

et al. 2016

Pharmaceutical Biology

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Context: Dillenia indica Linn. (Dilleniaceae) is traditionally used to treat skin inflammation. Objective: This study evaluated the healing effect of Dillenia indica fruit extracts on induced psoriasis-like wounds in Wistar rats. Materials and methods: Extracts were standardized to betulinic acid, including an aqueous ethanolic extract (AEE), ethyl acetate extract (EAE) and petroleum ether extract. Effects against lipid peroxidation were assessed in vitro. Wounds were created at rat tails (n = 12). Topical treatments were applied once daily for 7 days (1 mL of AEE or EAE at 5 or 50 mg/mL). Maximal dose was defined by the extract solubility. A 10-fold lower dose was also tested. Positive and negative controls were treated with clobetasol (0.5 mg/mL) or excipient. Half of each group was euthanized for histology. The remaining animals were observed for 20 days for wound measurements. Results: Yields of AEE and EAE were 4.3 and 0.7%, respectively. Betulinic acid concentrations in AEE and EAE were 4.6 and 107.6 mg/g. Extracts neutralized lipid peroxidation in vitro at 0.02 μg/mL, accelerating healing at 50 mg/mL. Complete healing in mice treated with AEE occurred 16 days after wound induction. This time was 14 and 12 days in mice treated with EAE and clobetasol. Compared to orthokeratosis, parakeratosis was reduced by AEE (25%), EAE (45%) and clobetasol (55%). EAE caused superior protection against biomolecules oxidation of skin compared to AEE. Discussion and conclusion: EAE exhibited activity closer to that of clobetasol. Betulinic acid may be an active constituent, which should be assessed in future studies.

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“…As Jheng et al (25) study showed a remarkable loss of brush border or tubular dilation, the present results revealed a significant increase in brush border loss, tubular dilation, vasodilatation and infiltration of leukocytes after induction of diabetic nephropathy by STZ-NA. The study of Lingaraju et al indicated that BA utilization was successful in ameliorating the renal histopathological changes induced by an experimental model of murine polymicrobial sepsis and one of the proposed influence mechanisms of BA in these changes were its antioxidant activity (6). Hence, due to the similarity of the present results with the study of Lingaraju et al, it could be suggested that renal protective effect of BA has been occurred through its antioxidant properties, but future studies are required to clarify the exact mechanism of this event.…”

Section: Discussionmentioning

confidence: 99%

“…Moreover, hepatoprotective effects of BA were found in HepG2 and hepatic stellate cells through its anti-inflammatory and antioxidant activity (5). Acute renal injury is associated with higher plasma Cr levels and widespread damage of tubules or epithelial necrosis that BA can attenuate them in pretreated mice (6). Additionally, renal ischemia reperfusion (I/R) increases serum levels of BUN and Cr in rats.…”

Section: Introductionmentioning

confidence: 99%

Preventive effects of betulinic acid on streptozotocinnicotinamide induced diabetic nephropathy in male mouse

et al. 2016

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Betulinic acid attenuates renal oxidative stress and inflammation in experimental model of murine polymicrobial sepsis

Cited by 40 publications

References 71 publications

Diarylheptanoids Rich Fraction ofAlnus nepalensisAttenuates Malaria Pathogenesis:In-vitroandIn-vivoStudy

Diarylheptanoids Rich Fraction ofAlnus nepalensisAttenuates Malaria Pathogenesis:In-vitroandIn-vivoStudy

Healing effect of Dillenia indica fruit extracts standardized to betulinic acid on ultraviolet radiation-induced psoriasis-like wounds in rats

Preventive effects of betulinic acid on streptozotocinnicotinamide induced diabetic nephropathy in male mouse

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