Betulin prevents high fat diet-induced non-alcoholic fatty liver disease by mitigating oxidative stress and upregulating Nrf2 and SIRT1 in rats

Farage, Amira E.; Abdo, Walied; Osman, Amira; Abdel-Kareem, Mona A.; Hakami, Zaki H.; Alsulimani, Ahmad; Bin-Ammar, Albandari; Alanazi, Ashwag S.; Alsuwayt, Bader; Alanazi, Mohammed M.; Antar, Samar A.; Kamel, Emadeldin M.; Mahmoud, Ayman M.

doi:10.1016/j.lfs.2023.121688

Cited by 5 publications

(1 citation statement)

References 65 publications

(96 reference statements)

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“…Under normal physiological conditions, Nrf2 binds to Keap1 in the cytoplasm and is maintained at low level by Keap1mediated ubiquitination and 26S proteasome-mediated degradation. When the body is stimulated by oxidative stress, Nrf2 will dissociate from Keap1, translocate to the nucleus, and bind with antioxidant response elements to promote the transcription of a variety of cellular defense molecules (27). In this study, western blot results showed that BFP-TA could increase the protein levels of SIRT1, Nrf2 and Keap1 in CSE-induced Beas-2B cells, suggesting that BFP-TA may attenuate the oxidative damage in CSE-induced Beas-2B cells by regulating the STRT1/ Nrf2/Keap1 signaling pathway.…”

Section: Discussionmentioning

confidence: 99%

Protective effect of the total alkaloid extract from Bulbus Fritillariae Pallidiflorae on cigarette smoke-induced Beas-2B cell injury model and transcriptomic analysis

Wang,

Liu,

AGA

et al. 2024

Food & Nutrition Research

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Background: Bulbus Fritillariae Pallidiflorae (BFP) is a traditional Chinese medicine that has long been used to treat lung diseases, but the active components and mechanism are still unclear. Objective: This study aimed to investigate the effect and mechanism of the total alkaloid extract from BFP (BFP-TA) on cigarette smoke extract (CSE)-induced Beas-2B cells injury. Design: The Beas-2B cells injury model was induced by 2% CSE, then the effect of BFP-TA on the levels of total antioxidant capacity (T-AOC), superoxide dismutase (SOD) and malondialdehyde (MDA) was detected according to the instructions of the T-AOC assay kit, the SOD detection kit and the MDA detection kit, and the production of ROS was detected by fluorescence microscopy. The effect of BFP-TA on Beas-2B cells apoptosis was detected by flow cytometry, and the effect of BFP-TA on related protein expression was detected by western blot. Subsequently, the effect of BFP-TA on differentially expressed genes (DEGs) in CSE-induced Beas-2B cells was studied by transcriptomic sequencing, and the expression of DEGs was verified by quantitative real-time polymerase chain reaction (qPCR). Results: The results showed that BFP-TA could attenuate CSE-induced oxidative damage in Beas-2B cells by elevating T-AOC and SOD levels while inhibiting ROS and MDA levels, and the mechanism was potentially related to the SIRT1/Nrf2/Keap1 signaling pathway. Furthermore, BFP-TA could inhibit CSE-induced apoptosis by inhibiting the protein expression of Bax, MST1 and FOXO3a, and exert anti-inflammatory effect by inhibiting the activation of MAPK signaling pathway. Subsequently, transcriptome analysis and qPCR validation showed that BFP-TA could alleviate inflammation, oxidative stress, apoptosis and lipid metabolism disorders by regulating the expression of DEGs in PPAR and PI3K-Akt signaling pathways, thereby exerting a protective effect against CSE-induced Beas-2B cell injury. Conclusion: This study is the first to demonstrate that BFP-TA could exert a protective effect on CSE-induced Beas-2B cell injury by exerting anti-inflammatory, antioxidant, anti-apoptotic and regulate lipid metabolism disorders.

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Section: Discussionmentioning

confidence: 99%

Protective effect of the total alkaloid extract from Bulbus Fritillariae Pallidiflorae on cigarette smoke-induced Beas-2B cell injury model and transcriptomic analysis

Wang,

Liu,

AGA

et al. 2024

Food & Nutrition Research

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Phytochemical Analysis and Anti‐dyslipidemia and Antioxidant Activities of Pluchea dioscoridis: In Vitro, In Silico and In Vivo Studies

Sultan,

Mahmoud,

Ahmed

et al. 2024

Chemistry & Biodiversity

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Pluchea dioscoridis is a flowering wild plant used traditionally in the treatment of rhematic disorders. This study investigated the phytochemical and in vitro radical scavenging activity (RSA), and in vivo anti‐hyperlipidemic, antioxidant and anti‐inflammatory properties of P. dioscoridis. The antihyperlipidemic efficacy was determined in a rat model of dyslipidemia. The extract and fractions of P. dioscoridis showed RSA with the EA fraction, exhibiting the most potent activity. The Phytochemical analysis of P. dioscoridis EA fraction (PDEAF) led to the isolation of five compounds (lupeol, quercetin, lupeol acetate, stigmasterol, and syringic acid). To evaluate its anti‐hyperlipidemic effect, dyslipidemia three doses of PDEAF were supplemented to rats for 14 days and poloxamer‐407 was administered on day 15 to induce dyslipidemia. All doses of PDEAF decreased plasma triglycerides, cholesterol, LDL and vLDL, and increased plasma LPL. PDEAF upregulated hepatic LDL receptor and suppressed HMG‐CoA reductase, decreased lipid peroxidation and TNF‐α and enhanced GSH and enzymatic antioxidants in dyslipidmeic rats. In silico findings revealed the binding affinity of the isolated compounds towards LPL, HMG‐CoA reductase, and LDL receptor. In conclusion, P. dioscoridis is rich in phytoconstituents, exhibited RSA and its EA fraction effectively prevented acute dyslipidemia and its associated oxidative stress and inflammatory response.

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miR-128-3p is involved in aluminum-induced cognitive impairment by regulating the Sirt1-Keap1/Nrf2 pathway

Li,

Liu,

Wang

et al. 2024

Ecotoxicology and Environmental Safety

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Betulin prevents high fat diet-induced non-alcoholic fatty liver disease by mitigating oxidative stress and upregulating Nrf2 and SIRT1 in rats

Cited by 5 publications

References 65 publications

Protective effect of the total alkaloid extract from Bulbus Fritillariae Pallidiflorae on cigarette smoke-induced Beas-2B cell injury model and transcriptomic analysis

Protective effect of the total alkaloid extract from Bulbus Fritillariae Pallidiflorae on cigarette smoke-induced Beas-2B cell injury model and transcriptomic analysis

Phytochemical Analysis and Anti‐dyslipidemia and Antioxidant Activities of Pluchea dioscoridis: In Vitro, In Silico and In Vivo Studies

miR-128-3p is involved in aluminum-induced cognitive impairment by regulating the Sirt1-Keap1/Nrf2 pathway

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