Betanodavirus infection in golden pompano, Trachinotus blochii, fingerlings cultured in deep-sea cage culture facility in Langkawi, Malaysia

Ransangan, Julian; Manin, Benny Obrain; Abdullah, Azila; Roli, Zuraidah; Sharudin, Erni F.

doi:10.1016/j.aquaculture.2011.02.040

Cited by 33 publications

(19 citation statements)

References 41 publications

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“…Thus, in 228 shellfish samples imported from both countries, a higher rate of BFNNV genotype was also detected compared to that of RGNNV. Some sporadic cases of VNN in farmed fish likely associated with RGNNV have been reported in the Asian countries (Gomez et al., , ; Ransangan, Manin, Abdullah, Roli, & Sharudin, ), but thus far, no laboratory has had confirmed cases of BFNNV. Nevertheless, the epidemiological investigations performed in this study clearly demonstrated the high prevalence rate of BFNNV in various shellfish species.…”

Section: Discussionmentioning

confidence: 99%

High prevalence of betanodavirus barfin flounder nervous necrosis virus as well as red‐spotted grouper nervous necrosis virus genotype in shellfish

Kim

Kwon

Kim

et al. 2017

Journal of Fish Diseases

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Using two serially executed PCRs, the discriminative multiplex two-step RT-PCR (DMT-2 RT-PCR) following the detection seminested two-step RT-PCR (DSN-2 RT-PCR), we found a high frequency presence of BFNNV genotype as well as RGNNV in various domestic and imported shellfish. This was definitely different from the previous reports of outbreaks and asymptomatic infection only by the RGNNV genotype in cultured finfish in Korea. Cultivation of NNV entrapped in shellfish was performed successfully by a blind passage. Thus, in an attempt to elucidate the epidemiology of betanodavirus, experiments conducted on 969 shellfish samples concluded that (i) distribution of NNV genotype, especially BFNNV, in shellfish is clearly different from that found in finfish of the world; (ii) unlike RGNNV, which showed a high rate in summer, BFNNV showed no seasonal variation and this result suggests BFNNVs in the marine environment remain fairly constant throughout the year; and (iii) the entrapped virus in shellfish was alive and culturable in vitro. These results are the first report of high level prevalence of in vitro culturable NNV in shellfish, for both BFNNV and RGNNV, which may present a potential risk in transmitting nodaviruses to host species in a marine environment.

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Section: Discussionmentioning

confidence: 99%

High prevalence of betanodavirus barfin flounder nervous necrosis virus as well as red‐spotted grouper nervous necrosis virus genotype in shellfish

Kim

Kwon

Kim

et al. 2017

Journal of Fish Diseases

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“…More recently, around 40 host species have been reported (22 families, 8 orders) which includes Atlantic cod (Gadus morhua); thread-sail filefish (Stephanolepis cirrhifer); spotted wolf fish (Anarhichas minor) sturgeon Acipenser sp., turbot (S. maximus); Chinese catfish (Parasilurus asotus), guppy (Poecilia reticulate), dragon grouper (Epinephelus lanceolatus), Japanese tilefish (Branchiostegus japonicas), firespot snapper (Lutjanus erythropterus), bluefin tuna (Thunnus thynnus); sevenband grouper (Epinephelus septemfasciatus), Golden pompano, (Trachinotus blochii) (E. septemfasciatus) and redspotted grouper (E. akaara) [46,54,69,86,98,100,103]. Larval or juvenile stages are more prone to this viral infection, but significant mortality can also occur in older fish up to production-size as in European seabass, sevenband grouper, humpback grouper Cromileptes altivelis and Atlantic halibut Hippoglossus hippoglossus [4,37,71,83,86,125].…”

Section: Occurrence and Distributionmentioning

confidence: 99%

“…Classical signs of disease are most commonly observed in larval and juvenile fishes since they are commonly affected [82] and sometimes in adult fish [37,71,103]. Diseased fish show various clinical symptoms which include reduced appetite, emaciation, colour change (darkening), abnormal (whirling) swimming pattern, neurological dysfunction, exophthalmia, swim bladder hyperinflation, floating belly up with inflation of swim bladder, anorexia and gas accumulation and extensive mortality ( Fig.…”

Section: Clinical Signsmentioning

confidence: 99%

Betanodavirus of Marine and Freshwater Fish: Distribution, Genomic Organization, Diagnosis and Control Measures

et al. 2012

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“…VNN in fish causes necrosis and vacuolation of the brain, spinal cord, and eyes [1], which leads to abnormal swimming behavior, and ultimately death. Massive disease outbreaks in Malaysia caused by VNN-associated viral infections have been reported in groupers ( Epinephelus fuscoguttatus ), Asian sea bass ( Lates calcarifer ), red snappers ( Lutjanus campechanus ), cobia ( Rachycentron canadum ), and golden pompano ( Trachinotus blochii ) [2-4]. Fishes in other Asian countries, such as Japan, Indonesia, Thailand, China, Singapore, and Taiwan, have also been affected by VNN [1].…”

Section: Introductionmentioning

confidence: 99%

Risk factors associated with viral nervous necrosis in hybrid groupers in Malaysia and the high similarity of its causative agent nervous necrosis virus to reassortant red-spotted grouper nervous necrosis virus/striped jack nervous necrosis virus strains

Ariff

Abdullah²,

Azmai

et al. 2019

Vet World

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Background and Aim: Viral nervous necrosis (VNN) is a serious disease of several marine fish species. VNN causes 100% mortality in the larval stages, while lower losses have been reported in juvenile and adult fish. This study aimed to detect the occurrence of VNN while identifying its associated risk factors and the genotypes of its causative agent in a hybrid grouper hatchery in Malaysia. Materials and Methods: A batch of newly hatched hybrid grouper fry (Epinephelus fuscoguttatus X Epinephelus lanceolatus) were followed from the larval stage to market size. Samples of the hybrid groupers, water, live feed, and artificial fish pellets were collected periodically from day 0 to 180 in the hybrid grouper hatchery. Reverse transcription-polymerase chain reaction (RT-PCR) and nested PCR amplifications were carried out on VNN-related sequences. The phylogenetic tree including the sampled causative agent of VNN was inferred from the coat protein genes from all known Betanodavirus species using Molecular Evolutionary Genetics Analysis (MEGA). Pearson's correlation coefficient values were calculated to determine the strength of the correlation between the presence of VNN in hybrid grouper samples and its associated risk factors. Results: A total of 113 out of 146 pooled and individual samples, including hybrid grouper, water, and artificial fish pellet samples, demonstrated positive results in tests for the presence of VNN-associated viruses. The clinical signs of infection observed in the samples included darkened skin, deformation of the backbone, abdominal distension, skin lesions, and fin erosion. VNN was present throughout the life stages of the hybrid groupers, with the first detection occurring at day 10. VNN-associated risk factors included water temperature, dissolved oxygen content, salinity, ammonia level, fish size (adults more at risk than younger stages), and life stage (age). Detection of VNN-associated viruses in water samples demonstrated evidence of horizontal transmission of the disease. All the nucleotide sequences found in this study had high nucleotide identities of 88% to 100% to each other, striped jack nervous necrosis virus (SJNNV), and the reassortant strain red-spotted grouper NNV/SJNNV (RGNNV/SJNNV) isolate 430.2004 (GenBank accession number JN189932.1) (n=26). The phylogenetic analysis showed that quasispecies was present in each VNN-causing virus-positive sample, which differed based on the type of sample and life stage. Conclusion: This study was the first to confirm the existence of a reassortant strain (RGNNV/SJNNV) in hybrid groupers from Malaysia and Southeast Asia. However, the association between the mode of transmission and the risk factors of this virus needs to be investigated further to understand the evolution and potential new host species of the reassortant strain. Keywords: epidemiology, quasispecies, reassortant, red-spotted grouper nervous necrosis virus-striped jack nervous necrosis virus, viral nervous necrosis.

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Betanodavirus infection in golden pompano, Trachinotus blochii, fingerlings cultured in deep-sea cage culture facility in Langkawi, Malaysia

Cited by 33 publications

References 41 publications

High prevalence of betanodavirus barfin flounder nervous necrosis virus as well as red‐spotted grouper nervous necrosis virus genotype in shellfish

High prevalence of betanodavirus barfin flounder nervous necrosis virus as well as red‐spotted grouper nervous necrosis virus genotype in shellfish

Betanodavirus of Marine and Freshwater Fish: Distribution, Genomic Organization, Diagnosis and Control Measures

Risk factors associated with viral nervous necrosis in hybrid groupers in Malaysia and the high similarity of its causative agent nervous necrosis virus to reassortant red-spotted grouper nervous necrosis virus/striped jack nervous necrosis virus strains

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