Betalactamasas: la evolución del problema

Astocondor-Salazar, Lilian

doi:10.35839/repis.2.2.224

Cited by 4 publications

(3 citation statements)

References 17 publications

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“…On the other hand, the acquisition of antibiotic resistance plasmids or insertion of resistant mobile genetic elements into the hvKp plasmid turns them into superbugs that can be termed hyper-resistant hvKp strains [ 8 – 10 ]. Some K. pneumoniae clones are characterized as high-risk clones that play an important role in the spread of antibiotic-resistant strains [ 11 , 12 ]. The association of the porin ompK36 with clonal relatedness of K. pneumoniae isolates has been described in several studies [ 13 , 14 ].…”

Section: Introductionmentioning

confidence: 99%

The face of hypervirulent Klebsiella pneumoniae isolated from clinical samples of two Iranian teaching hospitals

Sanikhani

Moeinirad

Solgi

et al. 2021

Ann Clin Microbiol Antimicrob

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Hypervirulent Klebsiella pneumoniae (hvKp) has emerged as a pathogen of global concern. In this study, both phenotypic and genotypic tests were used to detect hvKp. Antimicrobial resistance profiles and clonal relatedness of clinical isolates were also determined. We found that 34.2% (163/477) of the isolates were tellurite resistant, and among them 102 hvKp isolates detected with iucA or iutA or peg-344 as molecular markers. The blaSHV (80.4%), followed by blaCTX-M-15 (76.5%) and blaTEM (67.6%), blaOXA-48 (53.9%), and blaNDM-1 (32.3%) were detected, while blaKPC-1 was not present in any hvKp isolates. It was found that the majority of hvKp isolates belonged to capsular serotype K20 and ompK36 group C, which is related to clonal group (CG) 23 (e.g. ST23). A high percentage of multidrug-resistant hvKp (76.6%) and high resistance to imipenem (67%) indicated a serious problem that should be addressed in the clinical setting.

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Section: Introductionmentioning

confidence: 99%

The face of hypervirulent Klebsiella pneumoniae isolated from clinical samples of two Iranian teaching hospitals

Sanikhani

Moeinirad

Solgi

et al. 2021

Ann Clin Microbiol Antimicrob

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“…Las betalactamasas se diferencian por su espectro de resistencia, teniendo así las de espectro extendido (BLEE), las que se han descrito fundamentalmente en cepas de Escherichia coli, Klebsiella spp. y Enterobacter spp, aunque también en microorganismos no fermentadores como Pseudomonas aeruginosa la cual confiere resistencia a todos los antibióticos β-lactámicos con la excepción de las carbapenemas, las cefamicinas y las combinaciones de β-lactámicos con inhibidores de β-lactamasas (10,11).…”

Section: Introductionunclassified

Susceptibilidad antimicrobiana de bacterias causantes de infecciones del tracto urinario en mujeres y niñas

Barbecho Quizhpi,

Andrade Campoverde

2024

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Introducción. La infección del tracto urinario forma parte del grupo de las infecciones más frecuentes en los seres humanos, por lo que se la considera un problema de salud pública al presentar altas tasas de incidencia y morbilidad en la población pediátrica y adulta en diferentes regiones del mundo, siendo la mayor causa de estas de origen bacteriano, por lo cual los tratamientos se basan en la antibioticoterapia. Sin embargo, con el paso de los años se ha observado a nivel global el fracaso en los tratamientos empíricos debido a la resistencia bacteriana, presentando mayor incidencia países subdesarrollados. Objetivo. Caracterizar las diferentes especies bacterianas causantes de infección del tracto urinario en mujeres que acuden a consulta en la Fundación Humanitaria Pablo Jaramillo Crespo durante el año 2022, además identificar al agente causal más común y su clasificación dependiendo de si estos presentan o no la enzima betalactamasa de espectro extendido (BLEE). Metodología. Estudio descriptivo de cohorte transversal, documental secundario de muestreo no probabilístico por cobertura total. Resultados. De 304 urocultivos positivos se identificó un 62.5% casos de infección del tracto urinario en mujeres adultas y 37.5% en niñas, presentándose a Escherichia coli como el agente etiológico más común 82.2%, seguido de Proteus mirabilis 11.5%, Klebsiella pneumoniae 1.6% y 4.7% de otros microorganismos granmenegativos. Un 11.5% de casos positivos para la presencia de BLEE, siendo Escherichia coli el agente etiológico con más casos positivos para este mecanismo de resistencia con un mayor número de casos en la población infantil siendo de 21 casos. Conclusión. EL agente etiológico más común causante de Infección del tracto urinario en la población de esta casa de salud es Escherichia coli, presentándose un mayor porcentaje de casos de betalactamasa de espectro extendido en la población infantil, lo que demuestra la importancia de realizar un urocultivo y antibiograma para plantear un correcto esquema de tratamiento en los pacientes y así evitar que los microorganismos generen mecanismos de resistencia bacteriana. Área de estudio: Salud, Laboratorio clínico

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“…Carbapenemase production is an important mechanism underpinning bacterial resistance to carbapenem antibiotics. Carbapenemases are divided into three categories; A, B, and D Ambler classes [ 5 , 6 ]. Ambler class B carbapenemases are also called metallo-β-lactamases (MBLs).…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of a novel B1-β-lactamase gene, blaAFM-1 using a loop-mediated isothermal amplification (LAMP) assay

Qin

Duan

Peng

et al. 2021

Ann Clin Microbiol Antimicrob

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Background BlaAFM-1 (GenBank Accession No. 143105.1) is a new B1 subclass metallo-β-lactamase gene discovered by our group, and isolated from an Alcaligenes faecalis plasmid that renders carbapenem antibiotics ineffective. In this study, we generated a fast and reliable assay for blaAFM-1 detection. Methods We designed optimum loop-mediated isothermal amplification (LAMP) primers and constructed a recombinant plasmid AFM-1 to specifically detect blaAFM-1. Optimal LAMP primers were used to assess sensitivity of the recombinant plasmid AFM-1 and blaAFM-1-supplemented samples (simulated sputum and simulated feces). Fifty two samples, without blaAFM-1, were used to assess LAMP real-time assay specificity; these samples were verified by conventional PCR and sequencing for the absence of blaAFM-1. Three hundred clinical Gram-negative carbapenem-resistant strains were tested by LAMP assay for strains carrying blaAFM-1, which were confirmed by conventional PCR and Sanger sequencing. We calculated the sensitivity and its 95% confidence interval (95% CI), specificity and its 95% CI, and predictive values of the LAMP assay and conventional PCR/sequencing by investigating positive and negative clinical strains. Results The lowest limit of detection for the recombinant plasmid AFM-1 and blaAFM-1-supplemented samples (in both simulated sputum and simulated feces) was 101 copies/reaction. All amplification curves of the 52 blaAFM-1-free bacteria strains were negative, suggesting the LAMP assay had excellent specificity for detecting blaAFM-1. Among the 300 clinical strains, eight were positive for blaAFM-1 using LAMP. These LAMP results were consistent with conventional PCR and Sanger sequencing data. As with conventional PCR/sequencing, the LAMP method exhibits 100% sensitivity (95% CI 59.8–100%) and 100% specificity (95% CI 98.4–100%) for blaAFM-1 detection. The LAMP assay is also time-efficient (1 h) for blaAFM-1 detection. Conclusions We established a new LAMP assay with high sensitivity and specificity to detect the novel B1-β-lactamase gene, blaAFM-1.

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Betalactamasas: la evolución del problema

Cited by 4 publications

References 17 publications

The face of hypervirulent Klebsiella pneumoniae isolated from clinical samples of two Iranian teaching hospitals

The face of hypervirulent Klebsiella pneumoniae isolated from clinical samples of two Iranian teaching hospitals

Susceptibilidad antimicrobiana de bacterias causantes de infecciones del tracto urinario en mujeres y niñas

Rapid detection of a novel B1-β-lactamase gene, blaAFM-1 using a loop-mediated isothermal amplification (LAMP) assay

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