Betaine-rich sugar beet molasses protects from homocysteine-induced reduction of survival in Caenorhabditis elegans

Drobny, Alice; Meloh, Hedda; Wächtershäuser, Eike; Hellmann, Bernhard; Mueller, Andreas S.; Klis, J.D. van der; Fitzenberger, Elena; Wenzel, U.

doi:10.1007/s00394-019-01944-3

Cited by 6 publications

(5 citation statements)

References 27 publications

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“…[10,11] Betaine (N,N,N-trimethylglycine) is widely supplemented as a source of nutrition, which abundantly exists in sugar beets. [12] As an important methyl donor for methylation modification, betaine plays an important role in a variety of physiological activities. [13,14] High betaine intake during lactation increased milk betaine content and decreased adiposity in mouse offspring, which were accompanied by an incremental abundance of probiotic Akkermansia in the gut of mammalian neonates and an enduring increase in intestinal goblet cell number.…”

Section: Introductionmentioning

confidence: 99%

Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis

Chen

Liu

Mao

et al. 2022

Molecular Nutrition Food Res

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Scope: Betaine rich in beet is used as an important source of human nutrition. Here this study aims to explore whether betaine supplementation can protect against acute sever ulcerative colitis (ASUC) and the underlying mechanism Methods and results: ASUC model is induced by dextran sulfate sodium (DSS), and effects of betaine as a methyl donor on ASUC are evaluated. Betaine mitigates the changes, for example, elevated DAI, weight loss, spleen enlargement, colon shortening, and disordered colonic mucosa. The protective effects of betaine on colonic barrier integrity in ASUC are then verified through examining tight junction proteins by western blot and immunofluorescence. Spectrophotometry method and western blot confirm that betaine can decrease levels of oxidative markers (MDA, MPO, NOS, and COX2), and promote expressions of antioxidant proteins (GSH, NRF2, CAT, and SOD1). Further, betaine prevents colonic inflammatory pyroptosis by blocking expressions of NLRP3 inflammasome complex (NLRP3, ASC, and cleaved-caspase 1), N terminal-GSDMD, and release of relevant inflammatory factors. Conclusion: Betaine inhibits colonic oxidative stress induced inflammatory pyroptosis to alleviate ASUC, which shows therapeutic potential against colitis and other acute inflammatory disorder.

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Section: Introductionmentioning

confidence: 99%

Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis

Chen

Liu

Mao

et al. 2022

Molecular Nutrition Food Res

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“…Sugar beet ( Beta vulgaris L.) is a biennial plant that originated from the Mediterranean region and belongs to the Chenopodiacea family. The root of sugar beet is an abundant source of bioactive compounds, minerals, and vitamins (Filipčev et al, 2010 ; Koprivica et al, 2014 ; Mikołajczyk‐Bator et al, 2016 ), which provides health‐promoting effects such as the inhibition of tumor cell growth (Valli et al, 2012 ), protection from the oxidative damage (Filipčev et al, 2010 ; Mohdaly et al, 2010 ), and prevention of the age‐related diseases (Drobny et al, 2020 ). In Iranian traditional medicine, sugar beet has been considered as a medicinal root and is used to treat the psychiatric problems and strengthen the human immune system (El‐Gendy et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

“…

Mikołajczyk-Bator et al, 2016), which provides health-promoting effects such as the inhibition of tumor cell growth (Valli et al, 2012), protection from the oxidative damage (Filipčev et al, 2010;Mohdaly et al, 2010), and prevention of the age-related diseases (Drobny et al, 2020). In Iranian traditional medicine, sugar beet has been considered as a medicinal root and is used to treat the psychiatric problems and strengthen the human immune system (El-Gendy et al, 2022).

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confidence: 99%

Phenolic compounds of sugar beet (Beta vulgaris L.): Separation method, chemical characterization, and biological properties

Arjeh

Khodaei

Barzegar

et al. 2022

Food Science & Nutrition

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Sugar beet (Beta vulgaris L.) is a good source of bioactive compounds. However, information on the biological properties of sugar beet root is limited and its beneficial effects have not been completely understood. In this work, 10 phenolic compounds have been separated and identified in various parts of sugar beet for the first time, including the most abundant epicatechin (31.16 ± 1.89 mg/100 g), gallic acid (30.57 ± 2.69 mg/100 g), and quercetin‐3‐O‐rutinoside (30.14 ± 3.63 mg/100 g). The biological activity tests indicated that sugar beet peel potently scavenged the nitric oxide and DPPH (2,2‐diphenyl‐1‐picrylhydrazyl) free radicals with IC50 values of 88.17 ± 05.14 and 28.77 ± 0.62 μg/ml, respectively. In addition, sugar beet peel exhibited the highest reducing power, IC50 values of 11.98 ± 1.20 μg/ml, and the highest ion‐chelating activity, IC50 values of 48.52% and 55.21% for cupric and ferrous ions at 250 μg/ml, respectively. Compared to synthetic antioxidants, sugar beet showed promising biological activities, which could be considered further in future studies.

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“…2 ), Smek2 is probably involved in Sardh regulation. The Smek2 protein can regulate protein phosphatase 4 (PP4) 6 , which is required for expression of the stress response transcriptional regulator, DAF-16 29 , which in turn is required for high-homocysteine-stress resistance in the nematode C. elegans 30 . Another study has shown that Smek suppresses the expression of Wnt signaling genes by promoting histone deacetylase 31 .…”

Section: Discussionmentioning

confidence: 99%

Mutation in Smek2 regulating hepatic glucose metabolism causes hypersarcosinemia and hyperhomocysteinemia in rats

Tanaka

Kawano

Nakashima

et al. 2023

Sci Rep

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Suppressor of mek1 (Dictyostelium) homolog 2 (Smek2), was identified as one of the responsible genes for diet-induced hypercholesterolemia (DIHC) of exogenously hypercholesterolemic (ExHC) rats. A deletion mutation in Smek2 leads to DIHC via impaired glycolysis in the livers of ExHC rats. The intracellular role of Smek2 remains obscure. We used microarrays to investigate Smek2 functions with ExHC and ExHC.BN-Dihc2BN congenic rats that harbor a non-pathological Smek2 allele from Brown-Norway rats on an ExHC background. Microarray analysis revealed that Smek2 dysfunction leads to extremely low sarcosine dehydrogenase (Sardh) expression in the liver of ExHC rats. Sarcosine dehydrogenase demethylates sarcosine, a byproduct of homocysteine metabolism. The ExHC rats with dysfunctional Sardh developed hypersarcosinemia and homocysteinemia, a risk factor for atherosclerosis, with or without dietary cholesterol. The mRNA expression of Bhmt, a homocysteine metabolic enzyme and the hepatic content of betaine (trimethylglycine), a methyl donor for homocysteine methylation were low in ExHC rats. Results suggest that homocysteine metabolism rendered fragile by a shortage of betaine results in homocysteinemia, and that Smek2 dysfunction causes abnormalities in sarcosine and homocysteine metabolism.

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Betaine-rich sugar beet molasses protects from homocysteine-induced reduction of survival in Caenorhabditis elegans

Cited by 6 publications

References 27 publications

Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis

Betaine Ameliorates Acute Sever Ulcerative Colitis by Inhibiting Oxidative Stress Induced Inflammatory Pyroptosis

Phenolic compounds of sugar beet (Beta vulgaris L.): Separation method, chemical characterization, and biological properties

Mutation in Smek2 regulating hepatic glucose metabolism causes hypersarcosinemia and hyperhomocysteinemia in rats

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