Betaine ameliorates impaired steroidogenesis and apoptosis in mice granulosa cells induced by high glucose concentration

Samie, Kosar Abbasi; Tabandeh, Mohammad Reza; Afrough, Mahsa

doi:10.1080/19396368.2020.1811423

Cited by 15 publications

(11 citation statements)

References 34 publications

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“…In another study, berberine have also been found to prevent apoptosis of letrozole‐stimulated PCOS rat GCs in vivo and in vitro; optimize the balance of proapoptotic/antiapoptotic proteins, such as Bax, Bad, and Bcl‐2; and increase activation of PI3K/AKT signaling pathway (Yu et al, 2021). Betaine was reported to increase expression of antiapoptotic Bcl‐2 and decrease that of proapoptotic Bax and caspase‐3 in apoptotic GCs induced by high glucose concentrations (Samie et al, 2020). Furthermore, evodiamine is an indoloquinazoline alkaloid isolated from the fruits of Evodia rutaecarpa , which was reported to suppress the reduction of layers of GCs in PCOS rats at 4 and 40 mg/kg (Du & Yao, 2013).…”

Section: Natural Products and Bioactive Ingredients Of Tcm Inhibit Ap...mentioning

confidence: 99%

“…Betaine was reported to increase expression of antiapoptotic Bcl-2 and decrease that of proapoptotic Bax and caspase-3 in apoptotic GCs induced by high glucose concentrations (Samie et al, 2020). Furthermore, evodiamine is an indoloquinazoline alkaloid isolated from the fruits of Evodia rutaecarpa, which was reported to suppress the reduction of layers of GCs in PCOS rats at 4 and 40 mg/kg (Du & Yao, 2013).…”

Section: Alkaloidsmentioning

confidence: 99%

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The effects of natural products and bioactive ingredients of traditional Chinese medicine on apoptosis of ovarian granulosa cells

Zhong

Luo

Zhou

et al. 2022

J of Applied Toxicology

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Abnormal ovarian function is the main manifestation of female reproductive toxicity. Granulosa cells (GCs) play an important role in determining the fate of follicles and are the main effector cells of the female reproductive system. Excessive apoptosis of GCs leads to pathological folliculogenesis and further reproductive damage. However, drugs available for treatment of female reproductive toxicity are limited. Recent studies have confirmed that various natural products and bioactive ingredients of traditional Chinese medicine (TCM) can inhibit apoptosis of GCs and protect ovarian function. In this review, the mechanisms underlying the proapoptotic and antiapoptotic effects of natural products and bioactive ingredients of TCM on the proliferation, function, and apoptosis of GCs are summarized based on the findings of reports published over the past 10 years as reference for the treatment of female reproductive toxicity.

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Section: Natural Products and Bioactive Ingredients Of Tcm Inhibit Ap...mentioning

confidence: 99%

Section: Alkaloidsmentioning

confidence: 99%

The effects of natural products and bioactive ingredients of traditional Chinese medicine on apoptosis of ovarian granulosa cells

Zhong

Luo

Zhou

et al. 2022

J of Applied Toxicology

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“…9 Previous studies have revealed the inhibitory effects of elevated glucose on steroidogenesis of GCs, but the underlying mechanism remains unclear. 10,11 Recently, high glucose (HG) has been proved to activate NLR family PYRIN domain containing 3 (NLRP3) inflammasome and pyroptosis in various metabolic diseases, including diabetes and obesity-induced insulin resistance. 12,13 The NLRP3 inflammasome is a set of protein complex formed after recognizing a diverse group of stimuli, such as cell disruption, extracellular ATP, and pathogen-associated molecular patterns (PAMPs).…”

Section: Introductionmentioning

confidence: 99%

“…Higher glucose level in the follicular fluid (FF) was observed in such patients given the positive relationship between BMI and FF glucose concentration 9 . Previous studies have revealed the inhibitory effects of elevated glucose on steroidogenesis of GCs, but the underlying mechanism remains unclear 10,11 …”

Section: Introductionmentioning

confidence: 99%

Local glucose elevation activates pyroptosis via NLRP3 inflammasome in ovarian granulosa cells of overweight patients

Zhao

Jia

et al. 2023

The FASEB Journal

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Overweight, with an increasing prevalence worldwide, significantly impairs the clinical outcomes following in vitro fertilization (IVF). Hyperglycemia, hyperlipidemia, and metabolic disorders are always accompanied by the majority of overweight patients. The association between granulosa cell function and metabolic alterations in follicular fluid including lipids, proteins, and growth factors has been extensively documented. However, the effects of higher glucose level on ovarian granulosa cells (GCs), remain largely unknown. In this study, we identified that overweight women had elevated follicular glucose level which profoundly activated NLRP3 inflammasome and pyroptosis. An in vitro correlation between follicular high glucose, NLRP3 inflammasome and pyroptosis was also established. More importantly, in granulosa cells of overweight patients, the activation of the NLRP3 inflammasome and pyroptosis induced by high glucose was involved in the dysregulation of estradiol synthesis. Our study may provide new options to interpretate and improve IVF outcomes in overweight women.

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“…Exposure to hyperandrogen activated the NLRP3 inflammasome and derived ovarian dysfunction and fibrosis in mice [ 10 ]. High glucose induced NLRP3 expression in mouse GCs [ 11 ]. Non-esterified fatty acid induced NLRP3 inflammasome activation in GCs [ 12 ].…”

Section: Introductionmentioning

confidence: 99%

MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 1

Gao

et al. 2021

Bioengineered

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Polycystic ovary syndrome (PCOS) is a common endocrine disorder that poses a great threat to women's health. MiR-1224-5p is downregulated in the follicular fluid of patients with PCOS, but its role remains largely unknown. In this study, mice were treated with dehydroepiandrosterone (DHEA) to establish an in vivo model of PCOS. We found that enhanced activation of NLRP3 inflammasome was accompanied by downregulation of miR-1224-5p in ovarian tissue of PCOS mice. The effect of miR-1224-5p was further explored in TNF-α-treated human granulosa-like tumor (KGN) cells. Upregulation of miR-1224-5p suppressed TNF-α-induced secretion of DHEA and testosterone. MiR-1224-5p attenuated TNF-α-induced inflammation by inhibiting NLRP3 inflammasome activation, IL-1β synthesis, and nuclear factor kappa B (NF-κB) p65 nuclear translocation. Notably, miR-1224-5p decreased the expression of Forkhead box O 1 (FOXO1) and its downstream gene thioredoxin interaction protein (TXNIP). Luciferase reporter assay confirmed FOXO1 as a target of miR-1224-5p. Upregulation of FOXO1 abolished miR-1224-5p-induced activation of NLRP3 inflammasome, demonstrating that miR-1224-5p might inhibit NLRP3 inflammasome activation through regulating FOXO1. This study provided novel insights into the pathogenesis of PCOS and suggested that miR-1224-5p might be a promising target for treating PCOS.

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Betaine ameliorates impaired steroidogenesis and apoptosis in mice granulosa cells induced by high glucose concentration

Cited by 15 publications

References 34 publications

The effects of natural products and bioactive ingredients of traditional Chinese medicine on apoptosis of ovarian granulosa cells

The effects of natural products and bioactive ingredients of traditional Chinese medicine on apoptosis of ovarian granulosa cells

Local glucose elevation activates pyroptosis via NLRP3 inflammasome in ovarian granulosa cells of overweight patients

MiR-1224-5p attenuates polycystic ovary syndrome through inhibiting NOD-like receptor protein 3 inflammasome activation via targeting Forkhead box O 1

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