Betacyanin synthesis in red beet (Beta vulgaris) leaves induced by wounding and bacterial infiltration is preceded by an oxidative burst

Sepúlveda-Jiménez, Gabriela; Rueda-Benítez, Patricia; Porta, Helena; Rocha-Sosa, Mario

doi:10.1016/j.pmpp.2004.08.003

Cited by 76 publications

(44 citation statements)

References 39 publications

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“…Although some evidences suggest that betacyanins are able to provide protection against photoinhibition induced by high "white" and UV-A irradiances (Vogt et al 1999), little attention has been paid to their involvement in the defence against photo-damage caused by low temperature. The antioxidant properties of betacyanins have also been reported in some systems (Butera et al 2002, Cai et al 2003, Sepúlveda-Jiménez et al 2004, 2005. The production of betacyanins in S. salsa in response to H 2 O 2 treatments suggests that the pigment may function as a ROS scavenger, limiting the oxidative stress caused by environmental stressors (Wang et al 2006a).…”

Section: Discussionmentioning

confidence: 91%

Involvement of betacyanin in chilling-induced photoinhibition in leaves of Suaeda salsa

Wang¹,

Liu²

2007

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Seeds of Suaeda salsa were cultured in dark for 3 d and betacyanin accumulation in seedlings was promoted significantly. Then the seedlings with accumulated betacyanin (C+B) were transferred to 14/10 h light/dark and used for chilling treatment 15 d later. Photosystem 2 (PS2) photochemistry, D1 protein content, and xanthophyll cycle during the chilling-induced photoinhibition (exposed to 5 ºC at a moderate photon flux density of 500 µmol m -2 s -1 for 3 h) and the subsequent restoration were compared between the C+B seedlings and the control (C) ones. The maximal efficiency of PS2 photochemistry (F v /F m ), the efficiency of excitation energy capture by open PS2 centres (F v´/ F m´) , and the yield of PS2 electron transport (Ф PS2 ) of the C+B and C leaves both decreased during photoinhibition. However, smaller decreases in F v /F m , F v´/ F m´, and Ф PS2 were observed in the C+B leaves than in C ones. At the same time, the deepoxidation state of xanthophyll cycle, indicated by (A+Z)/(V+A+Z) ratio, increased rapidly but the D1 protein content decreased considerably during the photoinhibition. The increase in rate of (A+Z)/(V+A+Z) was higher but the D1 protein turnover was slower in C+B than C leaves. After photoinhibition treatment, the plants were transferred to a dim irradiation (10 μmol m -2 s -1 ) at 25 ºC for restoration. During restoration, the chlorophyll (Chl) fluorescence parameters, D1 protein content, and xanthophyll cycle components relaxed gradually, but the rate and level of restoration in the C+B leaves was greater than those in the C leaves. The addition of betacyanins to the thylakoid solution in vitro resulted in similar changes of F v /F m , D1 protein content, and (A+Z)/(V+A+Z) ratio during the chilling process. Therefore, betacyanin accumulation in S. salsa seedlings may result in higher resistance to photoinhibition, larger slowing down of D1 protein turnover, and enhancement of non-radiative energy dissipation associated with xanthophyll cycle, as well as in greater restoration after photoinhibition than in the control when subjected to chilling at moderate irradiance.Additional key words: chlorophyll fluorescence; D1 protein; hydrogen peroxide (H 2 O 2 ); photosystem 2; xanthophyll cycle.

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Section: Discussionmentioning

confidence: 91%

Involvement of betacyanin in chilling-induced photoinhibition in leaves of Suaeda salsa

Wang¹,

Liu²

2007

Photosynt.

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“…To our best knowledge, there are few reports on physiological roles of betalains in leaves of plants under environmental stresses. Previous studies indicated that the synthesis of betalains can be induced or stimulated by low temperature (Wang and Liu 2007), darkness, high salinity (Wang et al 2006), high UV radiation (Vogt et al 1999), exogenous H 2 O 2 treatment , and pathogen attack (Sepúlveda-Jiménez et al 2004). Betalains are proposed as the screening pigments that offer protection from UV-damage and pathogen attack, as an osmotic adjuster, as well as ROS-scavenger.…”

Section: Introductionmentioning

confidence: 99%

Comparison of thermostability of PSII between the chromatic and green leaf cultivars of Amaranthus tricolor L.

Zhan¹,

Shao²,

Huang³

et al. 2009

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In the present study, we investigated the antioxidative potential in leaves of the chromatic (CC) versus green (GC) Amaranthus tricolor L. under moderate high-temperature stress at 45°C. Before heat stress, CC had significantly higher levels of betacyanins [about 3.2 mg g -1 (FM)] than the green [1.8 mg g -1 (FM) (p<0.01), while similar chlorophyll (Chl) content [about 2 mg g -1 (FM)] was observed between both cultivars. After exposure to high temperature (45°C) for 6 days, betacyanins in leaves of CC were remarkably increased (about 2 times of that in control samples grown at 30°C). In contrast, betacyanins in GC significantly decreased by 56% in comparison with that of the control. Chl level in CC was higher than that in GC after heat stress for 6 days. Flavonoids and total phenolics in both cultivars were increased, but much more in CC. Significantly less H 2 O 2 accumulation was observed in the leaves and stems of CC than in those of GC under heat stress. Interestingly, much stronger circadian oscillation in fluorescence was observed in both cultivars after treatment at 45°C, which suggested that heat stress stimulates endogenous rhythms of photosystem II (PSII). Under moderate high-temperature stress, Chl fluorescence parameters F v /F m (maximum quantum yield of PSII), q P (coefficient of photochemical quenching), Φ PSII (effective PSII quantum yield), and ETR (electron transport rate) exhibited a gradual decrease, NPQ (nonphotochemical quenching) showed a slight increase followed by a gradual decline, whereas F o (minimum fluorescence of a dark-adapted leaf) increased continuously. In contrast to GC, after 120 h of high-temperature treatment, CC exhibited significantly lower F o level, and higher levels of F v /F m and NPQ. It is clear that PSII in CC was more stable than that in GC. The results indicate that betacyanins are an effective antioxidant, and probably contribute greatly to the higher thermal stability of PSII and higher tolerance to heat stress.Additional key words: Amaranthus tricolor L.; betacyanins accumulation; circadian oscillation; high temperature; thermal stability of PSII.

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“…The production of anthocyanins can be induced under abiotic stresses conditions such as high irradiance, temperature extremes, UV radiation, nutrient defi ciencies, dehydration and mechanical injury, as well as biotic stresses such as herbivory and pathogen attack (Gould, 2004). The production of betacyanin has also been reported to be induced under UV-A light exposure (Vogt et al, 1999), mechanical lesion and inoculation of pathogenic fungi (Sepúlveda-Jiménez et al, 2004). These same stressors lead to the generation of reactive oxygen species (ROS), indicating that betacyanin serves as an antioxidant to alleviate oxidative damage in the cells of S. japonica.…”

mentioning

confidence: 99%

Physiological Roles of Betacyanin in a Halophyte,Suaeda japonicaMakino

Hayakawa

Agarie

2010

Plant Production Science

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Betacyanin synthesis in red beet (Beta vulgaris) leaves induced by wounding and bacterial infiltration is preceded by an oxidative burst

Cited by 76 publications

References 39 publications

Involvement of betacyanin in chilling-induced photoinhibition in leaves of Suaeda salsa

Involvement of betacyanin in chilling-induced photoinhibition in leaves of Suaeda salsa

Comparison of thermostability of PSII between the chromatic and green leaf cultivars of Amaranthus tricolor L.

Physiological Roles of Betacyanin in a Halophyte,Suaeda japonicaMakino

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