Beta3-Adrenoceptor in the eel (<i>Anguilla anguilla</i>) heart:negative inotropy and NO-cGMP-dependent mechanism

Imbrogno, Sandra; Angelone, Tommaso; Adamo, C.; Pulerà, E.; Tota, Bruno; Cerra, Maria Carmela

doi:10.1242/jeb.02595

Cited by 49 publications

(50 citation statements)

References 44 publications

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“…We have previously shown that also in the working eel heart, intracardiac NO exerts an important modulation of mechanical performance via sGC signalling (see Imbrogno et al 2001Imbrogno et al , 2003Imbrogno et al , 2004Imbrogno et al , 2006. Of note, treatments with either a specific inhibitor of sGC ODQ (10 25 M), or with the cGMP-activated PKG inhibitor KT 5823 (10 26 M), or with cGMP analogue 8-Br-cGMP (10 26 M), or with KT 5720 (10 26 M) had no effects on the Frank -Starling response.…”

Section: Results (A) Effects Of S-nitroso-n-acetyl Penicillamine and mentioning

confidence: 99%

“…In the working eel heart, the NO-induced cGMP-PKG pathway remarkably modulates mechanical performance (see, for references, Tota et al 2005). For example, it tonically decreases SV either under basal condition (Imbrogno et al 2001) or following endoluminal chemical stimulations of AT 1 angiotensin II receptors (Imbrogno et al 2003), or b 3 adrenergic receptors (Imbrogno et al 2006), or exposure to the anti-adrenergic cardio-inhibitory peptide vasostatin I (Imbrogno et al 2004). Importantly, however, the present data strongly suggest that the cGMP-PKG mediated mechanisms are not involved in the eel heart under loading conditions, since the Frank-Starling response is not influenced by pre-treatment with either the sGC inhibitor ODQ or the cGMP analogue 8-Br-cGMP or the PKG antagonist KT 5823 .…”

Section: Discussionmentioning

confidence: 99%

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Phospholamban S-nitrosylation modulates Starling response in fish heart

et al. 2009

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The Frank -Starling mechanism is a fundamental property of the vertebrate heart, which allows the myocardium to respond to increased filling pressure with a more vigorous contraction of its lengthened fibres. In mammals, myocardial stretch increases cardiac nitric oxide (NO) release from both vascular endothelium and cardiomyocytes. This facilitates myocardial relaxation and ventricular diastolic distensibility, thus influencing the Frank -Starling mechanism.In the in vitro working heart of the eel Anguilla anguilla, we previously showed that an endogenous NO release affects the Frank -Starling response making the heart more sensitive to preload. Using the same bioassay, we now demonstrate that this effect is confirmed in the presence of the exogenous NO donor S-nitroso-N-acetyl penicillamine, is independent from endocardial endothelium and guanylate cyclase/ cGMP/protein kinase G and cAMP/protein kinase A pathways, involves a PI(3)kinase-mediated activation of endothelial NO synthase and a modulation of the SR-CA 2þ ATPase (SERCA2a) pumps. Furthermore, we show that NO influences cardiac response to preload through S-nitrosylation of phospholamban and consequent activation of SERCA2a. This suggests that in the fish heart NO modulates the Frank -Starling response through a beat-to-beat regulation of calcium reuptake and thus of myocardial relaxation.We propose that this mechanism represents an important evolutionary step for the stretch-induced intrinsic regulation of the vertebrate heart, providing, at the same time, a stimulus for mammalian-oriented studies.

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Section: Results (A) Effects Of S-nitroso-n-acetyl Penicillamine and mentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Phospholamban S-nitrosylation modulates Starling response in fish heart

et al. 2009

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“…In the eel, its role in mediating inotropic effects induced by cardioactive agents has been extensively demonstrated (Imbrogno et al, 2003;Imbrogno et al, 2004;Imbrogno et al, 2006). To analyse whether the CST-induced cardiac response involves a NO-cGMP pathway, the heart preparations were pretreated with either L-NMMA (10M; a NOS inhibitor) or ODQ (10M; a guanylyl cyclase blocker).…”

Section: G I/o Proteins and No-cgmp Signal Transduction Pathwaymentioning

confidence: 99%

“…) causes the classical positive inotropism in 70% of preparations and a negative inotropic effect in 30% of preparations (Imbrogno et al, 2006). The effect induced by ISO was studied after pretreating the hearts with CST.…”

Section: Cardioinhibitory Activity Of Cst On Adrenergic-stimulated Prmentioning

confidence: 99%

“…We previously reported that in the eel several humoral and neuroendocrine factors exert a cardio-inhibitory protection (Imbrogno et al, 2003;Imbrogno et al, 2004;Imbrogno et al, 2006). Among others, the NH 2 -terminal fragment of CgA, VS-1, has been shown to exert cardio-suppressive inotropic influences under both basal and beta-adrenergic-stimulated conditions (Imbrogno et al, 2004).…”

Section: Introductionmentioning

confidence: 99%

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The catecholamine release-inhibitory peptide catestatin (chromogranin A344-364) modulates myocardial function in fish

Imbrogno

Garofalo

Cerra

et al. 2010

Journal of Experimental Biology

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ISO-and endothelin-1-induced positive inotropism and coronary constriction . Thus, besides its antihypertensive properties, CST is now emerging as a novel cardiac modulator, which would protect the heart against excessive systemic and/or intra-cardiac excitatory stimuli (e.g. catecholamines and endothelin-1). Accepted 4 August 2010 SUMMARY Catestatin (CST), the 21-amino acid, cationic and hydrophobic peptide proteolytically derived from the ubiquitous chromogranin A (CgA), is an endogenous inhibitor of catecholamine release, a potent vasodilator in vivo and an anti-hypertensive agent in mammals, including humans. Recently, we discovered that CST also functions as an important negative modulator of heart performance in frog and rat. To gain an evolutionary perspective on CST cardiotropism in fish, we analysed the influence of bovine CST (CgA 344-364 ) on the eel heart, as well as the eventual species-specific mechanisms of its myocardial action. Experiments were carried out on fresh-water eels (Anguilla anguilla L.) using an electrically paced isolated working heart preparation.

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Fish Heart Growth and Function: From Gross Morphology to Cell Signaling and Back

Tota

Garofalo

2012

Ontogeny and Phylogeny of the Vertebrate Heart

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Beta3-Adrenoceptor in the eel (Anguilla anguilla) heart:negative inotropy and NO-cGMP-dependent mechanism

Abstract: Taken together, our findings provide functional evidence for the presence of ␤ ␤3-like adrenoceptors in the eel Anguilla anguilla heart identifying, for the first time in a working fish heart, the ␤ ␤3-AR-dependent negative inotropy discovered in mammals.

Cited by 49 publications

References 44 publications

Phospholamban S-nitrosylation modulates Starling response in fish heart

Phospholamban S-nitrosylation modulates Starling response in fish heart

The catecholamine release-inhibitory peptide catestatin (chromogranin A344-364) modulates myocardial function in fish

Fish Heart Growth and Function: From Gross Morphology to Cell Signaling and Back

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