Beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae have retained an intact right part of a Tn3-like transposon

Fayet, Olivier; Froment, Yvette; Piffaretti, Jean-Claude

doi:10.1128/jb.149.1.136-144.1982

Cited by 25 publications

(5 citation statements)

References 26 publications

(36 reference statements)

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“…This clearly shows that the Amp r phenotype of the three gonococcal strains PJ201, PJ202, and PJ203 is due to the presence of the 7.4 kb R plasmids. This was confirmed by further analysis which showed that these plasmids contain a 1.6 kb fragment closely related to the /3-1actamase coding region of the Tn 3-like transposon Tn2301 [14,15]. The 7.4 kb plasmids reported here are probably identical to the 7.1 kb plasmids described by others [1,3].…”

Section: Plasmid Content Of the Three Gonococcal Strains And Identifisupporting

confidence: 86%

Analysis of the plasmid content of three β-lactamase-producing Neisseria gonorrhoeae strains

Fayet¹

1982

FEMS Microbiology Letters

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Section: Plasmid Content Of the Three Gonococcal Strains And Identifisupporting

confidence: 86%

Analysis of the plasmid content of three β-lactamase-producing Neisseria gonorrhoeae strains

Fayet¹

1982

FEMS Microbiology Letters

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“…The prototypical 7.4 kb plasmid, often referred to as the Asia variant [14], contains nine loci that can be functionally grouped into Tn 2 -derived, replication, and mobilisation regions (). The plasmid contains 34 % of Tn 2 [44], including bla TEM , 84 % of the tnpR resolvase gene, and the right inverted repeat [45, 46].…”

Section: Resultsmentioning

confidence: 99%

“…1a). The plasmid contains 34 % of Tn2 [44], including bla TEM , 84 % of the tnpR resolvase gene, and the right inverted repeat [45,46].…”

Section: Characterisation Of Gonococcal Pblamentioning

confidence: 99%

There are three major Neisseria gonorrhoeae β-lactamase plasmid variants which are associated with specific lineages and carry distinct TEM alleles

et al. 2023

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Neisseria gonorrhoeae is a significant threat to global health with an estimated incidence of over 80 million cases each year and high levels of antimicrobial resistance. The gonococcal β-lactamase plasmid, pbla, carries the TEM β-lactamase, which requires only one or two amino acid changes to become an extended-spectrum β-lactamase (ESBL); this would render last resort treatments for gonorrhoea ineffective. Although pbla is not mobile, it can be transferred by the conjugative plasmid, pConj, found in N. gonorrhoeae . Seven variants of pbla have been described previously, but little is known about their frequency or distribution in the gonococcal population. We characterised sequences of pbla variants and devised a typing scheme, Ng_pblaST that allows their identification from whole genome short-read sequences. We implemented Ng_pblaST to assess the distribution of pbla variants in 15 532 gonococcal isolates. This demonstrated that only three pbla variants commonly circulate in gonococci, which together account for >99 % of sequences. The pbla variants carry different TEM alleles and are prevalent in distinct gonococcal lineages. Analysis of 2758 pbla-containing isolates revealed the co-occurrence of pbla with certain pConj types, indicating co-operativity between pbla and pConj variants in the spread of plasmid-mediated AMR in N. gonorrhoeae . Understanding the variation and distribution of pbla is essential for monitoring and predicting the spread of plasmid-mediated β-lactam resistance in N. gonorrhoeae .

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“…Restriction Maps of B-plusmids ( Fig. 3 and Table 2B) A comparison of the restriction endonuclease maps from the smaller (pSBOIB, pTorB) and the larger B-plasmids (pBenB, pRosB, p66 1 B, pBumB), shows that the smaller and the larger ones are homologous, except for a deletion of about 1.8 kb in the smaller opposite the TnAderived area (12,27). A region containing a DdeI fragment of 960 bp and a Hinfl site nearby is definitely missing in the smaller plasmids.…”

Section: Resultsmentioning

confidence: 99%

MOLECULAR CHARACTERIZATION AND COMPARISON OF PLASMID CONTENT IN SEVEN DIFFERENT STRAINS OF NEISSERIA GONORRHOEAE

Aalen

Gundersen

1987

Acta Pathologica Microbiologica Scandinavica Series B: Microbio

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The plasmid content of one penicillin sensitive and six penicillin resistant strains of Neisseria gonorrhoeae has been examined. All strains harbour a small, phenotypically cryptic plasmid of 4.1 kilo base pairs (kb). Four of the penicillin resistant strains carry a beta‐lactamase‐producing plasmid of 7.3 kb. One of these also carries a large plasmid of about 40 kb. The two remaining penicillin resistant strains harbour a smaller beta‐lactamase‐producing plasmid of 5.5 kb. The plasmids have been subjected to digestion with a number of restriction endonucleases, and their restriction maps have been compared. Judging by the maps, the cryptic (C‐) plasmids show great similarities. Except that two of them have 54 additional basepairs (bp), and two have a Hpall site instead of a Ddel site, no differences were found. The larger beta‐lactamase‐producing (B‐) plasmids have identical maps. The smaller seem to be homologous with the larger, except for a deletion of 1.8 kb. There is no correlation between the variant of C‐plasmid and type of B‐plasmid harboured in the penicillin resistant strains. The evolutionary implications suggested by this finding are discussed.

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Beta-lactamase-specifying plasmids isolated from Neisseria gonorrhoeae have retained an intact right part of a Tn3-like transposon

Cited by 25 publications

References 26 publications

Analysis of the plasmid content of three β-lactamase-producing Neisseria gonorrhoeae strains

Analysis of the plasmid content of three β-lactamase-producing Neisseria gonorrhoeae strains

There are three major Neisseria gonorrhoeae β-lactamase plasmid variants which are associated with specific lineages and carry distinct TEM alleles

MOLECULAR CHARACTERIZATION AND COMPARISON OF PLASMID CONTENT IN SEVEN DIFFERENT STRAINS OF NEISSERIA GONORRHOEAE

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