Beta-lactamase resistance genes in Enterobacteriaceae from Nigeria

Awosile, Babafela; Agbaje, Michael; Adebowale, Oluwawemimo; Kehinde, O. O.; Omoshaba, Ezekiel

doi:10.4102/ajlm.v11i1.1371

Cited by 6 publications

(8 citation statements)

References 82 publications

(125 reference statements)

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“…Studies point to a high prevalence among Enterobacteriaceae of the accumulation of genes from the bla family, mainly genes belonging to ESBL, OXA beta-lactamases and carbapenemases such as blaKPC and blaNDM . This phenomenon is more evident in hospital environments where selective pressure favors microorganisms carrying resistance genes and where there is a high rate of sharing of these genes [46, 47].…”

Section: Discussionmentioning

confidence: 99%

Molecular Insights into β-Lactams Resistance inKlebsiella pneumoniaeClinical Isolates with a Focus on Multidrug Resistance and Virulence

Nogueira,

Maia,

Clementino

et al. 2023

Preprint

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Klebsiella pneumoniae is associated with high resistance to antimicrobials and is common in isolates from colonization and nosocomial infections. The study aims to detect resistance genes belonging to the bla family and investigate metabolic pathways in K. pneumoniae isolates. Genes from the subfamilies included: blaSHV, blaTEM, blaNDM, blaKPC, blaGES, blaCTX-M and relevant variants of the blaOXA subfamily. Mass spectrometry data were acquired on the Orbitrap IDX spectrometer (Thermo) connected to the Vanquish UPLC system. Isolates from 122 K. pneumoniae samples were collected from 04/23/2019 to 05/29/2021. A high prevalence of resistance to penicillins, cephalosporins and carbapenems was found among the isolates. The identified genotypic profile showed a high prevalence of genes belonging to Ambler's classes of beta-lactamases A, B and D. In the metabolomic study, the N-fructosyl isoleucine metabolite was identified increased in multidrug-resistant (MDR) strains of K pneumoniae compared to strains susceptible to antimicrobials. In conclusion, the assays developed were efficient in detecting the main genes of the bla family of resistance in K. pneumoniae. The use of the pentose phosphate metabolic pathway suggests the regulation of bacterial growth, virulence, and colonization in MDR K. pneumoniae strains.

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Section: Discussionmentioning

confidence: 99%

Molecular Insights into β-Lactams Resistance inKlebsiella pneumoniaeClinical Isolates with a Focus on Multidrug Resistance and Virulence

Nogueira,

Maia,

Clementino

et al. 2023

Preprint

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“…The literature suggests that the CTX-M-15 gene variants are widely distributed in countries in Europe, North and South America as well as Asia [ 17 ]. It is important to note that the prevalence of CTX-M-producing E. coli varies between regions [ 10 , 18 ]. The prevalence rates of CTX-M-producing E. coli are at least 60% in Asia [ 10 ] while a lower rate of 34% has been reported in West Africa [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

“…It is important to note that the prevalence of CTX-M-producing E. coli varies between regions [ 10 , 18 ]. The prevalence rates of CTX-M-producing E. coli are at least 60% in Asia [ 10 ] while a lower rate of 34% has been reported in West Africa [ 18 ].…”

Section: Introductionmentioning

confidence: 99%

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Epidemiology of Cefotaxime-Hydrolysing β-Lactamase-Producing Escherichia coli in Children with Diarrhoea Reported Globally between 2012 and 2022

Karambwe,

Traoré,

Potgieter

2024

Microorganisms

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The global spread of cefotaxime-hydrolysing β-lactamase (CTX-M)-producing Escherichia coli (E. coli) and its associated impact on paediatric diarrhoeal treatment and management has become a public health concern. This review assessed surveillance studies on CTX-M-producing E. coli associated with diarrhoea in children published between 2012 and 2022 globally. A total of thirty-eight studies were included for data analysis, categorised into continental regions, and tabulated. The majority (68%) of studies were conducted in Asian countries while few studies were conducted in Europe (11%) and Africa (18%), respectively. On the African continent, the majority (11%) of studies were conducted in Northern Africa while no studies were reported in East Africa. On the American continent, 3% of the studies were reported from South America. The studies included were classified into diarrheagenic E. coli (74%; 28/38) and faecal carriage (26%; 10/38). Of all the E. coli pathotypes associated with CTX-M production, EPEC was frequently reported. The prevalence of CTX-M-producing E. coli including the CTX-M-15-producing variants ranged between 1% and 94%. About 37% of the studies generalised the report as blaCTX-M-positive E. coli. The use of sequencing in characterising the CTX-M-producing E. coli was reported in only 32% of all the studies. This review provides information on the epidemiology of CTX-M-15-producing E. coli in paediatric diarrhoea and the extent to which surveillance is being performed. This is relevant in informing clinical practice for the management of diarrhoea as well as the design of future surveillance studies.

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“…Most Escherichia coli and Pseudomonas aeruginosa resistance to beta-lactams is mainly due to the production of beta-lactamases enzymes, which are often encoded either on the chromosome or plasmid 2, 3, 4, (Ogba et al, 2022;Nomeh et al, 2023;Egwu et al, 2023). Beta-lactamases, such as carbapenemase betalactamases and extended-spectrum beta-lactamases (ESBLs), are increasingly being identified in food and companion animals, wildlife, humans, and the environment around the world 3,4,7,8 Joseph et al, 2023). The spread and convergence of multiple beta-lactamase genes across distinct resistant bacterial populations from various hosts and settings demonstrates that antimicrobial resistance (AMR) is a One Health issue 9 .…”

Section: Introductionmentioning

confidence: 99%

Characterization of VIM, VEB and CTX-M Beta-lactamase Gene in Escherichia coli and Pseudomonas aeruginosa Isolated from Urine Samples of Patients Visiting a Tertiary Hospital in Abakaliki

Ilang,

Peter,

Iroha

2023

Int J Med Sci Pharm Res

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The spread and convergence of multiple beta-lactamase genes across distinct resistant bacterial populations from various hosts and settings demonstrates increased risk of morbidity and mortality in humans. This study was undertaken to characterize blaVIM, blaVEB and blaCTX-M beta-lactamase gene in Escherichia coli and P. aeruginosa isolates from patients visiting a tertiary hospital in Abakaliki. A total of three hundred (300) urine samples were collected from patients and were subjected to bacteriological examination using culture, Gram staining and biochemical technique, for routine microbiological identification and further confirmed using the VITEK-2 Automated System (Biomerieux, France). Antimicrobial susceptibility studies were determined using the Kirby–Bauer disk diffusion method. All isolate were further screen for various beta-lactamase resistant gene by PCR using specific primer. Of the 300 urine samples collected, prevalence rate of 187 (62.3%) and 91 (30.3 %) E. coli and P. aeruginosa were recorded. The isolates exhibited 50.0-100% percentage of resistance to Amoxycillin-Clavulanic acid, Azetronam, Cefoxitin, Ceftriaxone and Piperacillin/tazobactam. The proportion of beta-lactamase gene in E. coli were as follows (VEB 143/76.5 %; CTX-M 175/93.5 %; VIM 77/41.2 %) while beta-lactamase gene in P. aeruginosa were as follows (VEB 91/100 %; CTX-M 63/69.2%; VIM 48/52.7 %). The presence of these gene in our study indicates the possibility of therapeutic failure, serious consequences for infection control and increased risk of morbidity and mortality in patients. Hence, continuous effort in hospital surveillance, infection control, and clinical audits must be conducted to fight against the rapid development and spread of antibiotic-resistant bacteria pathogens. Keywords: Beta-lactamase, Escherichia coli, Pseudomonas aeruginosa, VIM, VEB, CTX-M

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Beta-lactamase resistance genes in Enterobacteriaceae from Nigeria

Cited by 6 publications

References 82 publications

Molecular Insights into β-Lactams Resistance inKlebsiella pneumoniaeClinical Isolates with a Focus on Multidrug Resistance and Virulence

Molecular Insights into β-Lactams Resistance inKlebsiella pneumoniaeClinical Isolates with a Focus on Multidrug Resistance and Virulence

Epidemiology of Cefotaxime-Hydrolysing β-Lactamase-Producing Escherichia coli in Children with Diarrhoea Reported Globally between 2012 and 2022

Characterization of VIM, VEB and CTX-M Beta-lactamase Gene in Escherichia coli and Pseudomonas aeruginosa Isolated from Urine Samples of Patients Visiting a Tertiary Hospital in Abakaliki

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