Beta Cell Replacement Therapy

SoRelle, Jeffrey A.; Naziruddin, Bashoo

doi:10.5772/22283

Cited by 2 publications

(1 citation statement)

References 73 publications

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“…T1D requires life-long insulin therapy; however, several complications are associated with insulin injections, including hypoglycemic events and the persistence of macrovascular complications [ 2 , 3 ]. Therefore, beta cell replacement therapy is a promising treatment for T1D [ 4 ]. However, the success of this strategy is affected by a decrease in cell mass due to mechanical stress, a lack of oxygen, vascularization, an immediate blood-mediated immune response (IBMIR), and a lack of islet availability [ 5 ].…”

Section: Introductionmentioning

confidence: 99%

Functionalized Collagen/Poly(ethylene glycol) Diacrylate Interpenetrating Network Hydrogel Enhances Beta Pancreatic Cell Sustenance

Moreno-Castellanos

Cuartas-Gómez

Vargas-Ceballos

2023

Gels

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Three-dimensional matrices are a new strategy used to tackle type I diabetes, a chronic metabolic disease characterized by the destruction of beta pancreatic cells. Type I collagen is an abundant extracellular matrix (ECM), a component that has been used to support cell growth. However, pure collagen possesses some difficulties, including a low stiffness and strength and a high susceptibility to cell-mediated contraction. Therefore, we developed a collagen hydrogel with a poly (ethylene glycol) diacrylate (PEGDA) interpenetrating network (IPN), functionalized with vascular endothelial growth factor (VEGF) to mimic the pancreatic environment for the sustenance of beta pancreatic cells. We analyzed the physicochemical characteristics of the hydrogels and found that they were successfully synthesized. The mechanical behavior of the hydrogels improved with the addition of VEGF, and the swelling degree and the degradation were stable over time. In addition, it was found that 5 ng/mL VEGF-functionalized collagen/PEGDA IPN hydrogels sustained and enhanced the viability, proliferation, respiratory capacity, and functionality of beta pancreatic cells. Hence, this is a potential candidate for future preclinical evaluation, which may be favorable for diabetes treatment.

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