Beta-caryophyllene attenuates short-term recurrent seizure activity and blood-brain-barrier breakdown after pilocarpine-induced status epilepticus in rats

Mallmann, Michele Pereira; Mello, Fernanda Kulinski; Neuberger, Bruna; Sobral, Karine Gabriela da Costa; Fighera, Michele Rechia; Royes, Luiz Fernando Freire; Furian, Ana Flávia; Oliveira, Mauro Schneider

doi:10.1016/j.brainres.2022.147883

Cited by 6 publications

(5 citation statements)

References 42 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Animals were implanted with epidural electrodes following the protocol described in detail elsewhere. 29 After a 72-h recovery period, animals were subjected to pilocarpine-induced SE 29 and intranasal treatment. To this purpose, animals were treated with DRRSAb 2 μg/nostril or vehicle (NaCl 0.9%) 5 μL/nostril.…”

Section: Methodsmentioning

confidence: 99%

“…Animals were implanted with epidural electrodes following the protocol described in detail elsewhere . After a 72-h recovery period, animals were subjected to pilocarpine-induced SE and intranasal treatment.…”

Section: Methodsmentioning

confidence: 99%

“…Starting 7 days after the SE protocol, animals were assessed in a battery of behavioral tests. At day 7, animals were subjected to open field, neuroscore, and pick-up test to determine neuromotor performance, locomotion, and anxiety-like behavior. During the days 8 and 9, object recognition test, was carried out to evaluate the short-term and long-term memory.…”

Section: Methodsmentioning

confidence: 99%

“…One vehicle-and two DRRSAb-treated animals with artifactprone EEG recordings were excluded from quantitative EEG analysis. 29 to determine neuromotor performance, locomotion, and anxiety-like behavior. During the days 8 and 9, object recognition test, 31 was carried out to evaluate the short-term and long-term memory.…”

Section: Methodsmentioning

confidence: 99%

See 3 more Smart Citations

Electroencephalographic and Behavioral Effects of Intranasal Administration of a Na⁺, K⁺-ATPase-Activating Antibody after Status Epilepticus

Mello,

Sampaio,

Neuberger

et al. 2024

ACS Chem. Neurosci.

Self Cite

View full text Add to dashboard Cite

Status epilepticus (SE) is a medical emergency associated with high mortality and morbidity. Na + , K + -ATPase, is a promising therapeutic target for SE, given its critical role in regulation of neuron excitability and cellular homeostasis. We investigated the effects of a Na + , K + -ATPase-activating antibody (DRRSAb) on short-term electrophysiological and behavioral consequences of pilocarpine-induced SE. Rats were submitted to pilocarpine-induced SE, followed by intranasal administration (2 μg/nostril). The antibody increased EEG activity following SE, namely, EEG power in theta, beta, and gamma frequency bands, assessed by quantitative analysis of EEG power spectra. One week later, DRRSAb-treated animals displayed less behavioral hyperreactivity in pick-up tests and better performance in novel object recognition tests, indicating that the intranasal administration of this Na + , K + -ATPase activator immediately after SE improves behavioral outcomes at a later time point. These results suggest that Na + , K + -ATPase activation warrants further investigation as an adjunctive therapeutic strategy for SE.

show abstract

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

Electroencephalographic and Behavioral Effects of Intranasal Administration of a Na⁺, K⁺-ATPase-Activating Antibody after Status Epilepticus

Mello,

Sampaio,

Neuberger

et al. 2024

ACS Chem. Neurosci.

Self Cite

View full text Add to dashboard Cite

show abstract

“…Thus, like typical BBB-containing capillaries, fenestrated capillaries of the CVOs prevent parenchyma access to HMW molecules beyond the inner basement membrane. Blood-derived albumin in the brain parenchyma causes neurodegeneration ( Mallmann et al, 2022 ), demonstrating the neurotoxicity of blood-derived HMW molecules. However, this evidence does not proscribe all HMW molecule access to the parenchyma, as recent studies found that active transport of blood-derived HMW molecules into the brain parenchyma occurs by ligand-specific receptor-mediated and non-specific caveolar transcytosis ( Ellinger and Fuchs, 2010 ; Yang et al, 2020 ; Gonçalves and De Felice, 2021 ).…”

Section: General Introductionmentioning

confidence: 99%

Glial functions in the blood-brain communication at the circumventricular organs

Miyata

2022

Front. Neurosci.

View full text Add to dashboard Cite

The circumventricular organs (CVOs) are located around the brain ventricles, lack a blood-brain barrier (BBB) and sense blood-derived molecules. This review discusses recent advances in the importance of CVO functions, especially glial cells transferring periphery inflammation signals to the brain. The CVOs show size-limited vascular permeability, allowing the passage of molecules with molecular weight <10,000. This indicates that the lack of an endothelial cell barrier does not mean the free movement of blood-derived molecules into the CVO parenchyma. Astrocytes and tanycytes constitute a dense barrier at the distal CVO subdivision, preventing the free diffusion of blood-derived molecules into neighboring brain regions. Tanycytes in the CVOs mediate communication between cerebrospinal fluid and brain parenchyma via transcytosis. Microglia and macrophages of the CVOs are essential for transmitting peripheral information to other brain regions via toll-like receptor 2 (TLR2). Inhibition of TLR2 signaling or depletion of microglia and macrophages in the brain eliminates TLR2-dependent inflammatory responses. In contrast to TLR2, astrocytes and tanycytes in the CVOs of the brain are crucial for initiating lipopolysaccharide (LPS)-induced inflammatory responses via TLR4. Depletion of microglia and macrophages augments LPS-induced fever and chronic sickness responses. Microglia and macrophages in the CVOs are continuously activated, even under normal physiological conditions, as they exhibit activated morphology and express the M1/M2 marker proteins. Moreover, the microglial proliferation occurs in various regions, such as the hypothalamus, medulla oblongata, and telencephalon, with a marked increase in the CVOs, due to low-dose LPS administration, and after high-dose LPS administration, proliferation is seen in most brain regions, except for the cerebral cortex and hippocampus. A transient increase in the microglial population is beneficial during LPS-induced inflammation for attenuating sickness response. Transient receptor potential receptor vanilloid 1 expressed in astrocytes and tanycytes of the CVOs is responsible for thermoregulation upon exposure to a warm environment less than 37°C. Alternatively, Nax expressed in astrocytes and tanycytes of the CVOs is crucial for maintaining body fluid homeostasis. Thus, recent findings indicate that glial cells in the brain CVOs are essential for initiating neuroinflammatory responses and maintaining body fluid and thermal homeostasis.

show abstract

Beta-caryophyllene in psychiatric and neurological diseases: Role of blood-brain barrier

Mallmann,

Oliveira

2024

Blood-Brain Barrier

View full text Add to dashboard Cite

Beta-caryophyllene attenuates short-term recurrent seizure activity and blood-brain-barrier breakdown after pilocarpine-induced status epilepticus in rats

Cited by 6 publications

References 42 publications

Electroencephalographic and Behavioral Effects of Intranasal Administration of a Na⁺, K⁺-ATPase-Activating Antibody after Status Epilepticus

Electroencephalographic and Behavioral Effects of Intranasal Administration of a Na⁺, K⁺-ATPase-Activating Antibody after Status Epilepticus

Glial functions in the blood-brain communication at the circumventricular organs

Beta-caryophyllene in psychiatric and neurological diseases: Role of blood-brain barrier

Contact Info

Product

Resources

About

Beta-caryophyllene attenuates short-term recurrent seizure activity and blood-brain-barrier breakdown after pilocarpine-induced status epilepticus in rats

Cited by 6 publications

References 42 publications

Electroencephalographic and Behavioral Effects of Intranasal Administration of a Na+, K+-ATPase-Activating Antibody after Status Epilepticus

Electroencephalographic and Behavioral Effects of Intranasal Administration of a Na+, K+-ATPase-Activating Antibody after Status Epilepticus

Glial functions in the blood-brain communication at the circumventricular organs

Beta-caryophyllene in psychiatric and neurological diseases: Role of blood-brain barrier

Contact Info

Product

Resources

About

Electroencephalographic and Behavioral Effects of Intranasal Administration of a Na⁺, K⁺-ATPase-Activating Antibody after Status Epilepticus

Electroencephalographic and Behavioral Effects of Intranasal Administration of a Na⁺, K⁺-ATPase-Activating Antibody after Status Epilepticus