Bestimmung von Glykolyse-Metaboliten in isolierten Zellkernen

Metabolic Enzymes Moonlighting in the Nucleus: Metabolic Regulation of Gene Transcription

“…The initial identification of lactate in the nucleus [9] was followed by studies showing that phosphorylation of LDH [ 2 2 _ T D $ D I F F ] on Tyr238 is important for its nuclear translocation [42,55]. Nuclear LDH binds to DNA [56] and interacts with DNA polymerases /, d and e, stimulating DNA synthesis and post-UV radiation DNA repair [42].…”

“…The first reports of glycolytic enzymes present in the nucleus date to the late 1950s [8][9][10], but all essential glycolytic enzymes have now been observed in the nucleus [11] (Figure 1). Glycolysis produces pyruvate from glucose using the oxidative potential of NAD + [ 2 4 _ T D $ D I F F ] which is converted to NADH.…”