BestCyte® Cell Sorter Imaging System: Primary and adjudicative whole slide image rescreening review times of 500 ThinPrep Pap test thin-layers - An intra-observer, time-surrogate analysis of diagnostic confidence potentialities

BestCyte® primary screening of 500 ThinPrep Pap Test thin-layers: 3 Cytologists’ Interobserver diagnostic concordance with predicate manual microscopy relative to Truth Reference diagnoses defining NILM, ASCUS+, LSIL+, and ASCH+ thresholds for specificity, sensitivity, and equivalency grading

“… 1 Equally, innovative systems as the BestCyte® Cell Sorter Imaging System (BestCyte) (CellSolutions LLC, Greensboro, NC, USA) may facilitate efficient discrimination of abnormal epithelial cells in Pap test gynecological cytopathology. 2 , 3 , 4 …”

“…As digital imaging systems in cytopathology benefit directly from advancements in whole slide image (WSI) scanning technology and the availability of cost-efficient processing power, memory capacity, and networking capabilities, they may be primed for deployment in Pap test cervical cancer screening practice. 4 , 5 As BestCyte maps the cellular milieus on glass slides with precise conversion into digitized slides (i.e., WSI), it supports complex algorithmic computations on clinically relevant cells in fields of view (FOVs) to classify and strategically project images of cells in galleries based on cytomorphology. 3 , 4 BestCyte may therefore standardize objectivity between cytologists and inversely decrease interpretive subjectivity, hence diagnostic variance; thus, may prove superior to conventional manual microscopy (MM).…”

“… 4 , 5 As BestCyte maps the cellular milieus on glass slides with precise conversion into digitized slides (i.e., WSI), it supports complex algorithmic computations on clinically relevant cells in fields of view (FOVs) to classify and strategically project images of cells in galleries based on cytomorphology. 3 , 4 BestCyte may therefore standardize objectivity between cytologists and inversely decrease interpretive subjectivity, hence diagnostic variance; thus, may prove superior to conventional manual microscopy (MM). 3 , 4 , 6 Raised awareness of the advantages potentially availed from BestCyte technology is warranted as measured through interobserver diagnostic concordance, specificity, and sensitivity differentials, and cytologists’ equivalency grading relative to MM.…”

“… 3 , 4 BestCyte may therefore standardize objectivity between cytologists and inversely decrease interpretive subjectivity, hence diagnostic variance; thus, may prove superior to conventional manual microscopy (MM). 3 , 4 , 6 Raised awareness of the advantages potentially availed from BestCyte technology is warranted as measured through interobserver diagnostic concordance, specificity, and sensitivity differentials, and cytologists’ equivalency grading relative to MM.…”

“…In 2022, Chantziantoniou 4 explored assertions raised by Delga et al 3 by investigating BestCyte intraobserver primary screening outcomes as challenged by adjudicative WSI rescreening, whereby screening time formed a surrogate indicator for diagnostic confidence in overall system functionality. Chantziantoniou 4 likewise concluded BestCyte may be an effective screening device relative to MM based on its innovative design fostering favorable user learning curves and substantially reduced screening review time expenditures meanwhile maintaining diagnostic confidence. Likewise, that small dysplastic cells, as those arising from HISIL, may be efficiently detected and effectively differentiated from other cells in galleries as demonstrated by exceptional reproducibility kappa coefficients.…”