Best practices for evaluation of bone marrow in nonclinical toxicity studies

Reagan, William J.; Irizarry-Rovira, Armando R.; Poitout-Belissent, Florence; Bolliger, Anne Provencher; Ramaiah, Shashi K.; Travlos, Greg; Walker, Dana B.; Bounous, Denise I.; Walter, Gail L.

doi:10.1111/j.1939-165x.2011.00323.x

Cited by 47 publications

(53 citation statements)

References 55 publications

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“…During histopathological examination, the proportions of granulocytic to erythroid cells (or Myeloid to Erythroid ratio) were assessed (Wakenell, 2010;Reagan et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

Influence of dietary artemisinin supplementation on productive performance and haematological parameters of broiler chickens

et al. 2017

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-In the present study, we aimed to assess the toxicity of artemisinin on the haematological system and its effect on the productive performance of broiler chickens. Eighteen-day-old chickens were randomly divided into four groups of 30 chickens (three replicates of 10 broilers/group): control group and three experimental groups: ART5 -diet with 5 ppm of artemisinin; ART50 -diet with 50 ppm of artemisinin; and ART500 -diet with 500 ppm of artemisinin. Artemisinin enhanced the productive performances of broiler chickens at the lowest concentration (5 ppm), but at the highest concentration (500 ppm), it negatively affected weight gain and the feed conversion ratio. The performance characteristics of the chickens whose diets were supplemented with 50 ppm artemisinin were similar to those of the control group. Additionally, 5 ppm artemisinin in feed did not significantly affect the haematological parameters of the chickens, but 50 and 500 ppm artemisinin induced a gradual decline of the total leukocytes, lymphopenia, monocytosis, and eosinopenia, and the highest concentration caused anaemia. Artemisinin at a low concentration could be used as a feed additive in the poultry industry to improve organic broiler production performance with no serious side effects.

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“…During histopathological examination, the proportions of granulocytic to erythroid cells (or Myeloid to Erythroid ratio) were assessed (Wakenell, 2010;Reagan et al, 2011).…”

Section: Methodsmentioning

confidence: 99%

Influence of dietary artemisinin supplementation on productive performance and haematological parameters of broiler chickens

et al. 2017

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“…The studies are scrutinized by the Food and Drug Administration (FDA) and other agencies, and best practices are reviewed regularly. 117,164,184,251 Methods and data are accessible from the NTP Web site (http://ntp.niehs. nih.gov/) or from PubMed (http://www.ncbi.nlm.nih.gov/pubmed).…”

Section: Experimental Designmentioning

confidence: 99%

Pathobiology of Aging Mice and GEM

2012

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The use of induced and spontaneous mutant mice and genetically engineered mice (and combinations thereof) to study cancers and other aging phenotypes to advance improved functional human life spans will involve studies of aging mice. Genetic background contributes to pathology phenotypes and to causes of death as well as to longevity. Increased recognition of expected phenotypes, experimental variables that influence phenotypes and research outcomes, and experimental design options and rationales can maximize the utility of genetically engineered mice (GEM) models to translational research on aging. This review aims to provide resources to enhance the design and practice of chronic and longevity studies involving GEM. C57BL6, 129, and FVB/N strains are emphasized because of their widespread use in the generation of knockout, transgenic, and conditional mutant GEM. Resources are included also for pathology of other inbred strain families, including A, AKR, BALB/c, C3H, C57L, C58, CBA, DBA, GR, NOD.scid, SAMP, and SJL/J, and non-inbred mice, including 4WC, AB6F1, Ames dwarf, B6, 129, B6C3F1, BALB/c,129, Het3, nude, SENCAR, and several Swiss stocks. Experimental strategies for long-term cross-sectional and longitudinal studies to assess causes of or contributors to death, disease burden, spectrum of pathology phenotypes, longevity, and functional healthy life spans (health spans) are compared and discussed.

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“…(5) A comparison of the study type I sampling protocols with established recommendations for histopathological examinations in routine toxicity studies in rodents. For each organ/tissue, the similarities and differences between the type I study sampling protocols for porcine models, standard guidelines for organ sampling and trimming in rats and mice Morawietz et al 2004;Ruehl-Fehlert et al 2003), and applicable ''Best Practices'' guides of Working Groups of the STP are discussed (Bolon et al 2013;Haley et al 2005;Reagan et al 2011). (6) The recommended cross grid sizes for systematic random sampling procedures.…”

Section: Sampling Protocol Instructions and Illustrationsmentioning

confidence: 99%

Tissue Sampling Guides for Porcine Biomedical Models

et al. 2016

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This article provides guidelines for organ and tissue sampling adapted to porcine animal models in translational medical research. Detailed protocols for the determination of sampling locations and numbers as well as recommendations on the orientation, size, and trimming direction of samples from *50 different porcine organs and tissues are provided in the Supplementary Material. The proposed sampling protocols include the generation of samples suitable for subsequent qualitative and quantitative analyses, including cryohistology, paraffin, and plastic histology; immunohistochemistry; in situ hybridization; electron microscopy; and quantitative stereology as well as molecular analyses of DNA, RNA, proteins, metabolites, and electrolytes. With regard to the planned extent of sampling efforts, time, and personnel expenses, and dependent upon the scheduled analyses, different protocols are provided. These protocols are adjusted for (I) routine screenings, as used in general toxicity studies or in analyses of gene expression patterns or histopathological organ alterations, (II) advanced analyses of single organs/tissues, and (III) large-scale sampling procedures to be applied in biobank projects. Providing a robust reference for studies of porcine models, the described protocols will ensure the efficiency of sampling, the systematic recovery of high-quality samples representing the entire organ or tissue as well as the intra-/interstudy comparability and reproducibility of results.

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Best practices for evaluation of bone marrow in nonclinical toxicity studies

Cited by 47 publications

References 55 publications

Influence of dietary artemisinin supplementation on productive performance and haematological parameters of broiler chickens

Influence of dietary artemisinin supplementation on productive performance and haematological parameters of broiler chickens

Pathobiology of Aging Mice and GEM

Tissue Sampling Guides for Porcine Biomedical Models

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