Best Practices for Characterization of Magnetic Nanoparticles for Biomedical Applications

Sandler, Sarah E.; Fellows, Benjamin D.; Mefford, O. Thompson

doi:10.1021/acs.analchem.9b03518

Cited by 107 publications

(57 citation statements)

References 114 publications

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“…Hence, SOPs aim to homogenize procedures for characterizing physicochemical parameters of magnetic suspensions as the first step towards International Standards. So far, efforts with good results have been done to characterize and harmonize analysis methods for both suspended and immobilized MNPs [15,16,33,34]. Here we spotlight selected essential methods for MH application.…”

Section: Establishing Standard Operational Procedures For Structural mentioning

confidence: 99%

Whither Magnetic Hyperthermia? A Tentative Roadmap

et al. 2021

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The scientific community has made great efforts in advancing magnetic hyperthermia for the last two decades after going through a sizeable research lapse from its establishment. All the progress made in various topics ranging from nanoparticle synthesis to biocompatibilization and in vivo testing have been seeking to push the forefront towards some new clinical trials. As many, they did not go at the expected pace. Today, fruitful international cooperation and the wisdom gain after a careful analysis of the lessons learned from seminal clinical trials allow us to have a future with better guarantees for a more definitive takeoff of this genuine nanotherapy against cancer. Deliberately giving prominence to a number of critical aspects, this opinion review offers a blend of state-of-the-art hints and glimpses into the future of the therapy, considering the expected evolution of science and technology behind magnetic hyperthermia.

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Section: Establishing Standard Operational Procedures For Structural mentioning

confidence: 99%

Whither Magnetic Hyperthermia? A Tentative Roadmap

et al. 2021

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“…TEM is the most popular technique to measure nanoparticles' shape, size, and homogeneity because it provides direct images of the nanoparticles. Recent advances in TEM imaging, such as liquid-phase TEM, not only directly characterizes magnetic nanoparticles size and morphology but also characterizes the interparticle distances in a solution [79,80], which was shown to be a critical parameter for the magnetic response of the magnetic nanoparticles. Interestingly, TEM facilitates the real-time imaging for demonstrating the dynamic transformation in nanoparticles over time [81].…”

Section: Transmission Electron Microscopy (Tem)mentioning

confidence: 99%

A Guideline for Effectively Synthesizing and Characterizing Magnetic Nanoparticles for Advancing Nanobiotechnology: A Review

Kouhpanji

Stadler

2020

Sensors

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The remarkable multimodal functionalities of magnetic nanoparticles, conferred by their size and morphology, are very important in resolving challenges slowing the progression of nanobiotechnology. The rapid and revolutionary expansion of magnetic nanoparticles in nanobiotechnology, especially in nanomedicine and therapeutics, demands an overview of the current state of the art for synthesizing and characterizing magnetic nanoparticles. In this review, we explain the synthesis routes for tailoring the size, morphology, composition, and magnetic properties of the magnetic nanoparticles. The pros and cons of the most popularly used characterization techniques for determining the aforementioned parameters, with particular focus on nanomedicine and biosensing applications, are discussed. Moreover, we provide numerous biomedical applications and highlight their challenges and requirements that must be met using the magnetic nanoparticles to achieve the most effective outcomes. Finally, we conclude this review by providing an insight towards resolving the persisting challenges and the future directions. This review should be an excellent source of information for beginners in this field who are looking for a groundbreaking start but they have been overwhelmed by the volume of literature.

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“…The crystallinity and crystallite size of MNPs are important properties that interact strongly with the crystallization process itself as well as having a great influence on the end-use applications [51,52]. In this regard, XRD was employed to study the crystalline structure of MNPs before and after the immobilization process of N450D.…”

Section: Physiochemical Properties Of Mpns Aptes-coated Mnps and N45mentioning

confidence: 99%

Magnetic Cross-Linked Enzyme Aggregates of a Transpeptidase-Specialized Variant (N450D) of Bacillus licheniformis γ-Glutamyl Transpeptidase: An Efficient and Stable Biocatalyst for l-Theanine Synthesis

Chi

Huang

et al. 2021

Catalysts

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γ-Glutamyl transpeptidase (GGT) catalyzes the transfer of glutathione’s γ-glutamyl group and related γ-glutamyl amides to water, amino acids or peptides, and utilizes a conserved Thr residue to process its own polypeptide chain into a large and a small subunit that then assemble to produce a catalytically competent enzyme. In this study, the magnetic cross-linked enzyme aggregates (mCLEAs) of a transpeptidase-specialized variant (N450D) of Bacillus licheniformis GGT were successfully prepared with optimized process parameters viz.1.25:1 (v/v) of isopropanol to N450D (0.3 mg/mL) ratio/0.02:1 (w/w) of enzyme to 3-aminopropyl triethoxysilane (APTES)-coated magnetic nanoparticle ratio/20 mM of glutaraldehyde. The prepared magnetic nanoparticles and immobilized enzyme (N450D-mCLEAs) were characterized by X-ray diffraction (XRD) and Fourier transform infrared (FTIR) spectroscopy, field-emission scanning electron microscope integrated with energy dispersive X-ray spectroscopy (FESEM/EDS), and superparamagnetic analysis. As compared with free enzyme, N450D-mCLEAs displayed significantly higher heat resistance at temperatures of 55 and 60 °C, and had a greater stability over a storage period of one month. The immobilized enzyme could also be reused for 10 consecutive biocatalytic cycles with no significant reduction in the percent yield of l-theanine. Conclusively, this immobilization strategy surely provides a meaningful glance of developing N450D-mediated biocatalysis for the production of physiologically important γ-glutamyl compounds.

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Best Practices for Characterization of Magnetic Nanoparticles for Biomedical Applications

Cited by 107 publications

References 114 publications

Whither Magnetic Hyperthermia? A Tentative Roadmap

Whither Magnetic Hyperthermia? A Tentative Roadmap

A Guideline for Effectively Synthesizing and Characterizing Magnetic Nanoparticles for Advancing Nanobiotechnology: A Review

Magnetic Cross-Linked Enzyme Aggregates of a Transpeptidase-Specialized Variant (N450D) of Bacillus licheniformis γ-Glutamyl Transpeptidase: An Efficient and Stable Biocatalyst for l-Theanine Synthesis

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