Beryllium Metal Solubility in the Lung, Comparison of Metal and Hot-pressed Forms by in vivo and in vitro Dissolution Bioassays

Sonntag, A.; Métivier, Henri; Lantenois, G.; Boyer, M.; Nolibé, D.; Masse, R.

doi:10.1177/096032718700600311

Cited by 34 publications

(13 citation statements)

References 14 publications

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“…However, with two compounds ofberyllium, the translocation rates in rats and baboons were not comparable with the in vitro dissolution rates in lung fluid simulants (29). Similarly, the model of particle dissolution in lung fluid simulants did not hold for moderately soluble, uniform Co304 particles (14,(30)(31)(32).…”

Section: Introductionmentioning

confidence: 86%

Intracellular Particle Dissolution in Alveolar Macrophages

Kreyling¹

1992

Environmental Health Perspectives

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Aerosol particles deposited in the lungs that are not reaolily soluble in the epithelial lining fluid will be phagocytized by alveolar macrophages (AM). Inside the phagolysomal vacuole, the constituents ofthe plam allow dissolution ofavaiety ofcompounds at a higher rate than dissolutionietracelular lung fluids. Cielator concentration and a pH value ofabout 5 were found to control intracellular partice dissolution (IPD). Hence, IPD is the initial step oftranslocation ofdissolved material to blood, which is an important lung clearance nism for particles retined long term. IPD rtes of uniform test particles determined in human, baboon, and canine AM cultures were imilar to initialtrnslocation rates determined in lung clearnce studies ofthe same species after inhalation ofthe same test particles. IPD rate in cultured AM proved to be a sensitive functional parameter ofAM, which was used to identify changes in the clearance mechanism oftrnslocation during different exposure conditions.

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Section: Introductionmentioning

confidence: 86%

Intracellular Particle Dissolution in Alveolar Macrophages

Kreyling¹

1992

Environmental Health Perspectives

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“…However, alveolar macrophages can efficiently dissolve many inorganic particles with low solubility in water (5)(6)(7)(8)(9).…”

Section: Introductionmentioning

confidence: 99%

Phagolysosomal pH in alveolar macrophages.

Nyberg

Johansson

et al. 1992

Environ Health Perspect

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VW tdi phagolysm pH in veobr macrophags (AM) using fluorescein-labeledyead (FYP) amn silica particles (FSP) as probes. Fluorescence intensities from the ingested test particles were measured on populations of AM using fluorescence spectometry and on individual phagolysosomes using fluorescence microscopy. Measurements were performed on rabbit AM, which had been incubated with FYPorFSP (in vitot procedure). ealso insd FYPor FSP via the trachea into rabbit lungs and after 1 day, 1 week, 1 month, and 3 months lavaged thelungs and measured the pH in AM (in vivo procedure). Phagolysosomal pH was independent of the number and size of the fluorescent particles. Measurementsof uadons of AM with fluorescence spec y and of hd ual phagolysosomes with fluorescence microscopy gave similar average pH. For the FYP, pH decreased during the first day after lavage both in the in vit and the in vivo procedures. For the FSP, pH was unchaneddurngthe same period. After 1 daypH was sinilar for both particles. Electron microscopy showed a larger number of lysosomes in contact with phagosomes and a higher pementage ofvacuolated phagosomesforFYPthanforFSP. Inthein uwoprocedure, pH was unchanged at least upto 1 month, and this pH was lower than that in the in vitrv procedure. The difference was probably due to conditions at the time of phagocyosis Particles retained in the lung parenchyma were within AM, and their location within the AM appeared unchanged from 1 week up to 3 months.

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“…These studies emphasize the difference between the phagosome compartment of macrophages and epithelial cells, in particular their pH. Certain in vitro studies (45,46) have shown a good correlation with in vivo studies. However, in one study, using an acellular dissolution system (45) Certain factors have to be considered for the development of in vitro cell systemsthe particle dimensions, the nature of culture media, and the cell viability.…”

Section: Chemical Biopersisencementioning

confidence: 80%

In Vitro Assessment of Biopersistence Using Mammalian Cell Systems

Jaurand¹

1994

Environmental Health Perspectives

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Biopersistence of fibers in the respiratory airways is a concept including both the physical durability of the fibers and their chemical stability. Physical durability results from several events of diverse origins: fiber epuration by the lung clearance mechanisms, internalization by scavenger cells and fiber splitting. Fibers residing in the lung milieu will be attacked and modified chemically, structurally, and physically (size and shape). Fiber toxicity, which is very likely to be dependent on physical fiber characteristics, will also be dependent on the duration of the fiber's stay in the tissue. Biopersistence, therefore, will be a key issue in determining fiber toxicity. So far, few in vitro systems have been used to study parameters involved in biopersistence. However, examples exist of investigations of fiber phagocytosis by mammalian cells in culture, either by macrophages, or epithelial or mesothelial cells, and studies have also been reported of the fate of internalized fibers in relation to fiber dimensions and chemical stability, especially within macrophages and mesothelial cells. The methods will be presented and discussed to determine to what extent the development of in vitro biophysical models could help in determining those parameters, known or thought to be relevant to fiber persistence. -Environ Health Perspect 102(Suppl 5): 55-59 (1994)

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Beryllium Metal Solubility in the Lung, Comparison of Metal and Hot-pressed Forms by in vivo and in vitro Dissolution Bioassays

Cited by 34 publications

References 14 publications

Intracellular Particle Dissolution in Alveolar Macrophages

Intracellular Particle Dissolution in Alveolar Macrophages

Phagolysosomal pH in alveolar macrophages.

In Vitro Assessment of Biopersistence Using Mammalian Cell Systems

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