Berberine restored nitrergic and adrenergic function in mesenteric and iliac arteries from streptozotocin-induced diabetic rats

Zhao, Lili; Liu, Shuai; Wang, Man; Zhi, Minghua; Geng, Xufang; Hou, Cheng; Wang, Wei; Zhao, Dan

doi:10.1016/j.jep.2019.112140

Cited by 13 publications

(8 citation statements)

References 28 publications

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“…We have reported that BBR has protective effects in HUVECs damage induced by vanadium compounds, which is not mediated by eNOS, but related to reduced intracellular ROS [13]. And we also reported that in the STZ-induced early diabetic rats, neural control of mesenteric and iliac vasomotor tone are altered differently, the diminished nitrergic nerve on superior mesenteric artery and enhanced adrenergic nerve on iliac artery both contributed to increased vasoconstrictor responses; all these changes in diabetic rats were reversed by BBR [14]. Many studies have shown that metformin and BBR shares many aspects in actions and mechanisms [15,16].…”

supporting

confidence: 53%

“…After two weeks, the rats fed with HFD with obesity degree over 20% were determined to be successful in the obesity model (control group n=8, obesity model group n=8, BBR administration group n=8, metformin administration group n=8); then rats were concurrently treated with corresponding agents intragastrically as follows for two weeks: Control and obesity rats received equivalent amounts of saline solution, BBR administration group (200 mg/kg BBR), metformin administration group (100 mg/kg metformin). The dosages of BBR and metformin were adopted based on our laboratory and others previous studies [14,18,19]. All animals were handled in accordance with our institutional guidelines for animal care, as well as with the National Institutes of Health guidelines regarding the care and use of animals for experimental procedures.…”

Section: Methodsmentioning

confidence: 99%

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Berberine ameliorate mesenteric vascular dysfunction by perivascular adipose tissue on diet-induced obesity rats

Wang

Geng

et al. 2022

Preprint

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Background Berberine has been found to have anti-obesity effects, and obesity can lead to adipose tissue degeneration. As a special adipose tissue, perivascular adipose tissue (PVAT) is closely related to vascular function. What happens to PVAT in diet-induced obesity and how it affects vascular function are the focus of our experimental study. Methods Sprague-Dawley rats were fed with high fat diet (fat 34% kcal) for 4 weeks, simulated early obesity model. Obesity rats were treated with BBR (200 mg/kg) or metformin (100 mg/kg) by gavage for 2 weeks. The mesenteric arterioles were studied by atomic force microscopy (AFM), the Force vs time curves were observed and analyzed to indicate vascular function. Nitric oxide (NO) and noradrenaline (NA) released were quantified using organ bath with fluorescence assays and ELISA, respectively. Using network pharmacology to analyze the overlapping targets related of BBR and obesity-related diseases, and further analyzes the expression of NOS in mesenteric perivascular adipose tissue (PVAT) with immunohistochemistry and real-time PCR. The serum inflammatory factors levels were tested. Results BBR could significantly reduce the levels of blood glucose, blood lipid and inflammatory factors in serum, and effectively improved the abnormal mesenteric vasoconstriction and relaxation in obesity rats. There was no significant change in mesenteric vascular structure in obesity rats, but collagen fibers in mesenteric tissue were increased, and NO production and eNOS expression in mesenteric PVAT were increase, NA was decrease. All these changes in obesity rats on mesenteric tissue and PVAT were reversed by treated with BBR and metformin. Conclusions In diet-induced obesity rats, the function of vasoconstriction and relaxation in mesenteric arterioles are altered, NO is increased but NA is decreased in mesenteric PVAT. All these changes are reversed by BBR, suggesting a novel effects of BBR ameliorate mesenteric vascular dysfunction through regulating the PVAT, which may closely relate with anti-inflammatory and against oxidative stress effects.

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confidence: 53%

Section: Methodsmentioning

confidence: 99%

Berberine ameliorate mesenteric vascular dysfunction by perivascular adipose tissue on diet-induced obesity rats

Wang

Geng

et al. 2022

Preprint

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“…Both of these sites are predisposed to large vascular lesions in diabetes. Oral administration of 200 mg/kg berberine markedly enhanced the nitrergic neural activity in the superior mesenteric artery and diminished the adrenergic function in the iliac artery, resulting in vasodilatation in diabetic rats ( Geng et al, 2016 ; Zhao et al, 2019b ).…”

Section: Effects Of Berberine On Vascular Diseasesmentioning

confidence: 99%

Berberine: A Review of its Pharmacokinetics Properties and Therapeutic Potentials in Diverse Vascular Diseases

Peng

et al. 2021

Front. Pharmacol.

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Traditional Chinese medicine plays a significant role in the treatment of various diseases and has attracted increasing attention for clinical applications. Vascular diseases affecting vasculature in the heart, cerebrovascular disease, atherosclerosis, and diabetic complications have compromised quality of life for affected individuals and increase the burden on health care services. Berberine, a naturally occurring isoquinoline alkaloid form Rhizoma coptidis, is widely used in China as a folk medicine for its antibacterial and anti-inflammatory properties. Promisingly, an increasing number of studies have identified several cellular and molecular targets for berberine, indicating its potential as an alternative therapeutic strategy for vascular diseases, as well as providing novel evidence that supports the therapeutic potential of berberine to combat vascular diseases. The purpose of this review is to comprehensively and systematically describe the evidence for berberine as a therapeutic agent in vascular diseases, including its pharmacological effects, molecular mechanisms, and pharmacokinetics. According to data published so far, berberine shows remarkable anti-inflammatory, antioxidant, antiapoptotic, and antiautophagic activity via the regulation of multiple signaling pathways, including AMP-activated protein kinase (AMPK), nuclear factor κB (NF-κB), mitogen-activated protein kinase silent information regulator 1 (SIRT-1), hypoxia-inducible factor 1α (HIF-1α), vascular endothelial growth factor phosphoinositide 3-kinase (PI3K), protein kinase B (Akt), janus kinase 2 (JAK-2), Ca2+ channels, and endoplasmic reticulum stress. Moreover, we discuss the existing limitations of berberine in the treatment of vascular diseases, and give corresponding measures. In addition, we propose some research perspectives and challenges, and provide a solid evidence base from which further studies can excavate novel effective drugs from Chinese medicine monomers.

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“…Then, the rats were concurrently treated with the corresponding agents intragastrically as follows for two weeks: the control and obese rats received equivalent amounts of saline solution, the BBR administration group was administered 200 mg/kg BBR (25 mg/mL, Sichuan Xieli Pharmaceutical Co., Ltd), and the MET administration group was administered 100 mg/kg MET (12.5 mg/mL, Shouguang Fukang Pharmaceutical Co., Ltd). The dosages of BBR and MET were adopted based on reports from our laboratory and other previous studies [ 15 , 20 , 21 ]. All animals were handled in accordance with our institutional guidelines for animal care, as well as with the National Institutes of Health guidelines regarding the care and use of animals for experimental procedures.…”

Section: Methodsmentioning

confidence: 99%

“…We also reported that in the early stages of STZ-induced diabetes in rats, neural control of mesenteric and iliac vasomotor tone are altered differently, and the diminished nitrergic nerve on the superior mesenteric artery and enhanced adrenergic nerve on the iliac artery both contributed to increased vasoconstrictor responses. All these changes in diabetic rats were reversed by BBR [ 15 ]. Many studies have shown that MET and BBR share many aspects in their actions and mechanisms [ 16 – 18 ].…”

Section: Introductionmentioning

confidence: 99%

Berberine ameliorates mesenteric vascular dysfunction by modulating perivascular adipose tissue in diet-induced obese in rats

Wang

Geng

et al. 2022

BMC Complement Med Ther

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Background Berberine (BBR) has been found to have antiobesity effects, and obesity can lead to adipose tissue degeneration. As a special adipose tissue, perivascular adipose tissue (PVAT) is closely related to vascular function and affects vasoconstriction and relaxation. What happens to PVAT in the early stages of diet-induced obesity and how BBR affects vascular function is the focus of our experimental study. Methods Sprague–Dawley rats were fed a high-fat diet (fat 34% kcal) for 4 weeks to simulate early obesity. Obese rats were treated with BBR (200 mg/kg) or metformin (MET, 100 mg/kg) by gavage for 2 weeks. The mesenteric arterioles were studied by atomic force microscopy (AFM). The force vs. time curves were observed and analysed to indicate vascular function. Nitric oxide (NO) and noradrenaline (NA) release was quantified using an organ bath with fluorescence assays and ELISA, respectively. Network pharmacology was used to analyse the overlapping targets related to BBR and obesity-related diseases, and the expression of NOS in mesenteric PVAT was further analysed with immunohistochemistry and real-time PCR. The serum inflammatory factor levels were tested. Results BBR significantly reduced the levels of blood glucose, blood lipids and inflammatory factors in serum. It also effectively improved abnormal mesenteric vasoconstriction and relaxation in obese rats. There was no significant change in mesenteric vascular structure, but NO production and eNOS expression were significantly increased in mesenteric PVAT (P < 0.01), and NA was decreased (P < 0.05) in obese rats. All these changes in the mesenteric arterioles and PVAT of obese rats were reversed by treatment with BBR and MET. Conclusions In diet-induced obesity in rats, the function of vasoconstriction and relaxation in mesenteric arterioles is altered, NO is increased, and NA is decreased in mesenteric PVAT. All these changes were reversed by BBR, suggesting a novel effect of BBR in ameliorating mesenteric vascular dysfunction by regulating PVAT.

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Berberine restored nitrergic and adrenergic function in mesenteric and iliac arteries from streptozotocin-induced diabetic rats

Cited by 13 publications

References 28 publications

Berberine ameliorate mesenteric vascular dysfunction by perivascular adipose tissue on diet-induced obesity rats

Berberine ameliorate mesenteric vascular dysfunction by perivascular adipose tissue on diet-induced obesity rats

Berberine: A Review of its Pharmacokinetics Properties and Therapeutic Potentials in Diverse Vascular Diseases

Berberine ameliorates mesenteric vascular dysfunction by modulating perivascular adipose tissue in diet-induced obese in rats

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