Berberine protects against ischemia/reperfusion injury after orthotopic liver transplantation via activating Sirt1/FoxO3α induced autophagy

Lin, Yuanbang; Sheng, Mingwei; Weng, Yiqi; Xu, Rubin; Lü, Ning; Du, Hongyin; Yu, Wenli

doi:10.1016/j.bbrc.2017.01.028

Cited by 52 publications

(47 citation statements)

References 30 publications

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“…Inhibiting mitochondrial fission protects the liver against I/R injury. Other studies have shown that inhibiting mitochondrial fission protects the heart against I/R injury . Continuous mitochondrial division and fusion require a constant and well‐regulated phospholipid supply to ensure membrane integrity.…”

Section: Discussionmentioning

confidence: 99%

Ursodeoxycholyl lysophosphatidylethanolamide protects against hepatic ischemia/reperfusion injury via phospholipid metabolism‐mediated mitochondrial quality control

Zhang

Guo

et al. 2020

FASEB j.

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Mitochondrial dysfunction is the leading cause of reactive oxygen species (ROS) burst and apoptosis in hepatic ischemia/reperfusion (I/R) injury. Ursodeoxycholyl lysophosphatidylethanolamide (UDCA-LPE) is a hepatotargeted agent that exerts hepatoprotective roles by regulating lipid metabolism. Our previous studies have shown that UDCA-LPE improves hepatic I/R injury by inhibiting apoptosis and inflammation. However, the role of UDCA-LPE in lipid metabolism and mitochondrial function in hepatic I/R remains unknown. In the present study, we investigated the role of UDCA-LPE in hepatic I/R by focusing on the interface of phospholipid metabolism and mitochondrial homeostasis. Livers from 28-week-old mice, primary hepatocytes and HepG2 cells were subjected to in vivo and in vitro I/R, respectively.Analyses of oxidative stress, imaging, ATP generation, genetics, and lipidomics showed that I/R was associated with mitochondrial dysfunction and a reduction in phospholipids. UDCA-LPE alleviated mitochondria-dependent oxidative stress and apoptosis and prevented the decrease of phospholipid levels. Our study found that cytosolic phospholipase A 2 (cPLA 2 ), a phospholipase that is activated during I/R, hydrolyzed mitochondrial membrane phospholipids and led to mitochondria-mediated oxidative stress and apoptosis. UDCA-LPE inhibited the interaction between cPLA 2 and mitochondria and reduced phospholipid hydrolysis-mediated injury. UDCA-LPE might regulate the crosstalk between the phospholipid metabolism and the mitochondria, restore mitochondrial function and ameliorate I/R injury. K E Y W O R D ScPLA 2 , defect mitochondria, mitochondrial membrane, oxidative stress, phospholipid metabolism disorders, reperfusion injury | 6199 GU et al.

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Section: Discussionmentioning

confidence: 99%

Ursodeoxycholyl lysophosphatidylethanolamide protects against hepatic ischemia/reperfusion injury via phospholipid metabolism‐mediated mitochondrial quality control

Zhang

Guo

et al. 2020

FASEB j.

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“…Mitochondria constantly undergo fusion and fission for the maintenance of organelle fidelity . Mitochondrial fission and ensuing fragmentation are triggers for apoptosis, a critical pathogenic mechanism of IRI .…”

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confidence: 99%

“…Therefore, developing effective therapeutic strategies for I/R-induced liver injury is urgently required. (1) Recent evidence suggests that apoptosis is a major mode of hepatocyte death caused by IRI, (2,3) where abnormal mitochondrial fission is greatly involved. (4)(5)(6) Mitochondria constantly undergo fusion and fission for the maintenance of organelle fidelity.…”

mentioning

confidence: 99%

“…(4)(5)(6) Mitochondria constantly undergo fusion and fission for the maintenance of organelle fidelity. (3,7,8) Mitochondrial fission and ensuing fragmentation are triggers for apoptosis, a critical pathogenic mechanism of IRI. (9) Current research on mitochondrial involvement in I/R damage has focused mainly on the relationship between mitochondrial morphology and metabolic function rather than on apoptosis.…”

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confidence: 99%

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Hepatic stimulator substance resists hepatic ischemia/reperfusion injury by regulating Drp1 translocation and activation

Zhang

Huang

2017

Hepatology

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“…11 Besides the function of anti-inflammatory, berberine has the functions of hepatoprotection, improving cognitive impairment, treating atherosclerosis, protecting against neurotoxicity, anti-diabetic nephropathy effect. [12][13][14][15][16][17][18][19][20] Palmatine also has broad biological activities, such as hepatoprotective effect, anti-breast cancer, chondroprotective and cardioprotective effect, etc. [21][22][23][24][25] Previously, isoquinoline alkaloids from Phellodendri Cortex were isolated and purified by conventional techniques, such as polyamide chromatography, sephadex LH-20, SI and silica gel column chromatography, which were a waste of organic solvent, tedious, time-consuming.…”

Section: Introductionmentioning

confidence: 99%

Preparative Separation of Isoquinoline Alkaloids From Phellodendri Cortex by Ph‑zone-Refining Counter-Current Chromatography

Tian¹,

Cui²,

Yan³

et al. 2018

Quim. Nova

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publicado na web em 18/01/2018 pH-Zone-refining counter-current chromatography (PZRCCC) was successfully used for preparative separation isoquinoline alkaloids from Phellodendri Cortex. Two target compounds of berberine and palmatine were completely resolved by a two-phase solvent system chloroform/methanol/water (4:2:2, v/v), with 10 mmol L -1 HCl in upper stationary phase and 10 mmol L -1 TEA in lower organic phase. From 2.0 g of the crude alkaloids, palmatine (448 mg, 95.1%) and berberine (159 mg, 95.4%) were obtained in one-step PZRCCC separation. The chemical structures were identified by ESI-MS and NMR data. The results indicated that PZRCCC separation is an excellent preparative separation method for isoquinoline alkaloids from Phellodendri Cortex.

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Berberine protects against ischemia/reperfusion injury after orthotopic liver transplantation via activating Sirt1/FoxO3α induced autophagy

Cited by 52 publications

References 30 publications

Ursodeoxycholyl lysophosphatidylethanolamide protects against hepatic ischemia/reperfusion injury via phospholipid metabolism‐mediated mitochondrial quality control

Ursodeoxycholyl lysophosphatidylethanolamide protects against hepatic ischemia/reperfusion injury via phospholipid metabolism‐mediated mitochondrial quality control

Hepatic stimulator substance resists hepatic ischemia/reperfusion injury by regulating Drp1 translocation and activation

Preparative Separation of Isoquinoline Alkaloids From Phellodendri Cortex by Ph‑zone-Refining Counter-Current Chromatography

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