Berberine prevents stress-induced gut inflammation and visceral hypersensitivity and reduces intestinal motility in rats

Yu, Zhichao; Cen, Yong-Xin; Wu, Ben-Hua; Wei, Cheng; Xiong, Feng; Li, Defeng; Liu, Tingting; Luo, Ming‐Han; Guo, Liliangzi; Li, Yingxue; Wang, Lisheng; Wang, Jianyao; Yao, Jun

doi:10.3748/wjg.v25.i29.3956

Cited by 34 publications

(27 citation statements)

References 63 publications

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“…The reason for these targeted reductions is likely multi-layered. Psychological stress increases gut motility 84,85 , which may increase shear stress on mucosal-adhered microbial groups. In addition, given the fact that the microbiota exist in climax communities, alterations in mucosal immune function, nutrient availability, or epithelial barrier stability could all have drastic effects on microbiome structure 86,87 .…”

Section: Discussionmentioning

confidence: 99%

Unique maternal immune and functional microbial profiles during prenatal stress

Antonson

Evans

Galley

et al. 2020

Sci Rep

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Maternal stress during pregnancy is widespread and is associated with poor offspring outcomes, including long-term mental health issues. Prenatal stress-induced fetal neuroinflammation is thought to underlie aberrant neurodevelopment and to derive from a disruption in intrauterine immune homeostasis, though the exact origins are incompletely defined. We aimed to identify divergent immune and microbial metagenome profiles of stressed gestating mice that may trigger detrimental inflammatory signaling at the maternal–fetal interface. In response to stress, maternal glucocorticoid circuit activation corresponded with indicators of systemic immunosuppression. At the maternal–fetal interface, density of placental mononuclear leukocytes decreased with stress, yet maternal whole blood leukocyte analysis indicated monocytosis and classical M1 phenotypic shifts. Genome-resolved microbial metagenomic analyses revealed reductions in genes, microbial strains, and metabolic pathways in stressed dams that are primarily associated with pro-inflammatory function. In particular, disrupted Parasutterella excrementihominis appears to be integral to inflammatory and metabolic dysregulation during prenatal stress. Overall, these perturbations in maternal immunological and microbial regulation during pregnancy may displace immune equilibrium at the maternal–fetal interface. Notably, the absence of and reduction in overt maternal inflammation during stress indicates that the signaling patterns driving fetal outcomes in this context are more nuanced and complex than originally anticipated.

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Section: Discussionmentioning

confidence: 99%

Unique maternal immune and functional microbial profiles during prenatal stress

Antonson

Evans

Galley

et al. 2020

Sci Rep

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“…Previous studies have shown that BBH has potential anti-inflammatory, antibacterial, analgesic, and antidiarrheal effects and a wide range of biological activities, especially in regulating the release of some neurotransmitters, which are involved in the pathological process of various neurological diseases. A recent study ( Yu et al, 2019 ) has shown that intestinal immune disorders are closely related to the pathogenesis of IBS. Cytokines are a part of intestinal immune regulation.…”

Section: Discussionmentioning

confidence: 99%

Therapeutic Effects of Berberine Hydrochloride on Stress-Induced Diarrhea-Predominant Irritable Bowel Syndrome Rats by Inhibiting Neurotransmission in Colonic Smooth Muscle

Huang

Zhang

et al. 2021

Front. Pharmacol.

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The etiology of diarrhea-predominant irritable bowel syndrome (IBS-D) is complicated and closely related to neurotransmission in the gastrointestinal (GI) tract. Developing new strategies for treating this disease is a major challenge for IBS-D research. Berberine hydrochloride (BBH), the derivative of berberine, is a herbal constituent used to treat IBS. Previous studies have shown that BBH has potential anti-inflammatory, antibacterial, analgesic, and antidiarrheal effects and a wide range of biological activities, especially in regulating the release of some neurotransmitters. A modified IBS-D rat model induced by chronic restraint stress was used in all experiments to study the effects of BBH on the GI tract. This study measured the abdominal withdrawal reflex (AWR) response to graded colorectal distention (CRD; 20, 40, 60, and 80 mmHg) and observed the fecal areas of stress-induced IBS-D model. Experiments were conducted using organ bath techniques, which were performed in vitro using strips of colonic longitudinal smooth muscle. Inhibitory and excitatory neurotransmitter agents were added to each organ bath to observe contractile responses on the strips and the treatment effect exerted by BBH. The IBS-D rat model was successfully induced by chronic restraint stress, which resulted in an increased defecation frequency and visceral hypersensitivity similar to that of humans. BBH could reduce 4-h fecal areas and AWR response to CRD in IBS-D. The stress-induced IBS-D model showed upregulated colonic mRNA expression levels of 5-hydroxytryptamine-3A receptor and downregulated expression levels of neuronal nitric oxide synthase. Meanwhile, BBH could reverse this outcome. The responses of substances that regulate the contraction induced by related neurotransmission in the longitudinal smooth muscle of IBS-D colon (including the agonist of acetylcholine, carbachol; NOS inhibitor, L-NAME; and P2Y1 receptor antagonist, MRS2500) can be inhibited by BBH. In summary, BBH promotes defecation frequency and visceral hypersensitivity in IBS-D and exerts inhibitory effects on contractile responses in colonic longitudinal smooth muscle. Thus, BBH may represent a new therapeutic approach for treating IBS-D.

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“…The reason for these targeted reductions is likely multi-layered. Psychological stress increases gut motility 82,83 , which may increase shear stress on mucosal-adhered microbial groups. In addition, given the fact that the microbiota exist in climax communities, alterations in mucosal immune function, nutrient availability, or epithelial barrier stability could all have drastic effects on microbiome structure 84,85 .…”

Section: Discussionmentioning

confidence: 99%

Unique maternal immune and functional microbial profiles during prenatal stress

Antonson

Evans

Galley

et al. 2020

Preprint

View full text Add to dashboard Cite

Maternal stress during pregnancy is widespread and stress-induced fetal neuroinflammation is thought to derive from a disruption in intrauterine immune homeostasis, though the exact origins are incompletely defined. We aimed to identify divergent immune and microbial metagenome profiles of stressed gestating mice that may underlie detrimental inflammatory signaling at the maternal-fetal interface. In response to stress, maternal glucocorticoid circuit activation corresponded with diminished spleen mass and IL-1β production, reflecting systemic immunosuppression. At the maternal-fetal interface, density of placental mononuclear leukocytes decreased with stress. Yet maternal whole blood leukocyte analysis indicated monocytosis and classical M1 phenotypic shifts. Genome-resolved microbial metagenomic analyses revealed reductions in genes, microbial strains, and metabolic pathways in stressed dams that are primarily associated with pro-inflammatory function. Overall, these data indicate that stress disrupts maternal immunological and microbial regulation during pregnancy, characterized by concurrent anti-and pro-inflammatory signatures, which may displace immune equilibrium at the maternal-fetal interface.

show abstract

Berberine prevents stress-induced gut inflammation and visceral hypersensitivity and reduces intestinal motility in rats

Cited by 34 publications

References 63 publications

Unique maternal immune and functional microbial profiles during prenatal stress

Unique maternal immune and functional microbial profiles during prenatal stress

Therapeutic Effects of Berberine Hydrochloride on Stress-Induced Diarrhea-Predominant Irritable Bowel Syndrome Rats by Inhibiting Neurotransmission in Colonic Smooth Muscle

Unique maternal immune and functional microbial profiles during prenatal stress

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