Berberine-mediated up-regulation of surfactant protein D facilitates cartilage repair by modulating immune responses via the inhibition of TLR4/NF-ĸB signaling

Zhou, Yan; Jiang, Ming; Deng, Ming; Li, Yaming; Li, Bochun; Li, Jia; Ma, Yonggang; Chen, Zhonghui; Liu, Shiqing

doi:10.1016/j.phrs.2020.104690

Cited by 22 publications

(12 citation statements)

References 37 publications

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“…NF-κB regulates the expression of genes involved in inflammatory responses, and several studies have shown that inhibition of NF-κB pathway can decrease the pathogenesis of OA and RA [25,26,61,62]. Recently, several TCM compounds including VA have shown potential in inhibiting inflammatory response through inhibition of different parts of the NF-κB pathway [48,[63][64][65][66][67][68][69][70]. An in vitro study on lipopolysaccharide (LPS)-stimulated mouse peritoneal macrophages showed that VA had anti-inflammatory effects that were mediated by the inhibition of LPS-induced NF-κB activation and IκB-α degradation [71].…”

Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Chondroprotective Effects of Vanillic Acid and Epimedin C in Human Osteoarthritic Chondrocytes

et al. 2020

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In osteoarthritis (OA), inhibition of excessively expressed pro-inflammatory cytokines in the OA joint and increasing the anabolism for cartilage regeneration are necessary. In this ex-vivo study, we used an inflammatory model of human OA chondrocytes microtissues, consisting of treatment with cytokines (interleukin 1β (IL-1β)/tumor necrosis factor α (TNF-α)) with or without supplementation of six herbal compounds with previously identified chondroprotective effect. The compounds were assessed for their capacity to modulate the key catabolic and anabolic factors using several molecular analyses. We selectively investigated the mechanism of action of the two most potent compounds Vanillic acid (VA) and Epimedin C (Epi C). After identification of the anti-inflammatory and anabolic properties of VA and Epi C, the Ingenuity Pathway Analysis showed that in both treatment groups, osteoarthritic signaling pathways were inhibited. In the treatment group with VA, nuclear factor kappa-light-chain-enhancer of activated B cells (NF-κB) signaling was inhibited by attenuation of the nuclear factor of kappa light polypeptide gene enhancer in B-cells inhibitor alpha (IκBα) phosphorylation. Epi C showed a significant anabolic effect by increasing the expression of collagenous and non-collagenous matrix proteins. In conclusion, VA, through inhibition of phosphorylation in NF-κB signaling pathway and Epi C, by increasing the expression of extracellular matrix components, showed significant anti-inflammatory and anabolic properties and might be potentially used in combination to treat or prevent joint OA.

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Section: Discussionmentioning

confidence: 99%

Anti-Inflammatory and Chondroprotective Effects of Vanillic Acid and Epimedin C in Human Osteoarthritic Chondrocytes

et al. 2020

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“…Zhang et al revealed that mesenchymal stem cell exosomes mediate cartilage repair by enhancing proliferation, a t t e n u a t i n g a p o p t o s i s a n d m o d u l a t i n g i m m u n e reactivity (29). Zhou et al reported that Berberine-mediated release of surfactant protein D (SP-D) promoted cartilage repair by modulating immune responses via the inhibition of TLR4/NF-ĸB signaling (30). Bone remodeling is closely related to osteoblast differentiation and osteoclast resorption, which are important processes in the treatment of osteoporosis.…”

Section: Discussionmentioning

confidence: 99%

AEG-1 deletion promotes cartilage repair and modulates bone remodeling-related cytokines via TLR4/MyD88/NF-κB inhibition in ovariectomized rats with osteoporosis

Zhang¹,

Zhao²

2020

Ann Transl Med

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Background: Osteoporosis is a systemic skeletal disorder that can impact a variety of bones throughout the body. Astrocyte-elevated gene-1 (AEG-1) is involved in multiple pro-tumorigenic functions and participates in various inflammatory reactions. However, whether it has an impact on osteoporosis-related cartilage repair and bone remodeling remains unknown. Methods:We utilized an ovariectomy mouse model with AEG-1 deletion to investigate the role of AEG-1 in osteoporosis. The mRNA level of AEG-1 was detected by RT-PCR, bone markers, bone volume/total volume (BV/TV), trabecular bone surface/bone volume (BSA/BV) and trabecular bone thickness (Tb. Th) were detected by micro computed tomography (μCT), bone injury was observed by HE and alcian blue staining.The contents of IL-6, IL-17, iNOS and IL-10 in peripheral blood of the three groups were detected by ELISA.The expression of OSX, coi1a1, OC, TLR4, MyD88 and NF-κB were detected by Western Blot.Results: μCT revealed increased bone volume in the AEG-1 knockout (KO) ovariectomy (OVX) group compared to the wildtype (WT) OVX group 4 weeks after surgery, indicating restored bone formation after AEG-1 deletion. Flow sorting revealed that AEG-1 deletion inhibited the production of inflammatory factors. Western blot demonstrated activation of the TLR4/MyD88/NF-κB pathway after LPS exposure, which was reduced by AEG-1 deletion. AEG-1 deletion also improved lipopolysaccharide (LPS) induced adverse reactions.Conclusions: Taken together, these findings indicate that AEG-1 deletion improves cartilage repair and bone remodeling during osteoporosis, which may partly occur through the inhibition of the TLR4/MyD88/ NF-κB signaling pathway.

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“…It has been shown that berberine inhibits chondrocyte apoptosis and cartilage degradation via activating AMPK signaling and suppressing p38 MAPK activity [24,25]. Berberine also decreases inflammation and cartilage degradation by modulating the host immune response through the inhibition of TLR4/NF-κB signaling [26]. Moreover, berberine has been associated with bone formation by promoting osteogenic differentiation via activation of Runx-2 and p38 MAPK and reducing osteoclast differentiation [27,28].…”

Section: Alkaloids Berberinementioning

confidence: 99%

Emerging Natural-Product-Based Treatments for the Management of Osteoarthritis

et al. 2021

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Osteoarthritis (OA) is a complex degenerative disease in which joint homeostasis is disrupted, leading to synovial inflammation, cartilage degradation, subchondral bone remodeling, and resulting in pain and joint disability. Yet, the development of new treatment strategies to restore the equilibrium of the osteoarthritic joint remains a challenge. Numerous studies have revealed that dietary components and/or natural products have anti-inflammatory, antioxidant, anti-bone-resorption, and anabolic potential and have received much attention toward the development of new therapeutic strategies for OA treatment. In the present review, we provide an overview of current and emerging natural-product-based research treatments for OA management by drawing attention to experimental, pre-clinical, and clinical models. Herein, we review current and emerging natural-product-based research treatments for OA management.

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Berberine-mediated up-regulation of surfactant protein D facilitates cartilage repair by modulating immune responses via the inhibition of TLR4/NF-ĸB signaling

Cited by 22 publications

References 37 publications

Anti-Inflammatory and Chondroprotective Effects of Vanillic Acid and Epimedin C in Human Osteoarthritic Chondrocytes

Anti-Inflammatory and Chondroprotective Effects of Vanillic Acid and Epimedin C in Human Osteoarthritic Chondrocytes

AEG-1 deletion promotes cartilage repair and modulates bone remodeling-related cytokines via TLR4/MyD88/NF-κB inhibition in ovariectomized rats with osteoporosis

Emerging Natural-Product-Based Treatments for the Management of Osteoarthritis

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