Berberine improves intestinal epithelial tight junctions by upregulating A20 expression in IBS-D mice

Hou, Qiuke; Zhu, Shuilian; Zhang, Changrong; Huang, Yongquan; Guo, Yajuan; Li, Peiwu; Chen, Xinlin; Wen, Yi; Han, Quan‐Bin; Liu, Fengbin

doi:10.1016/j.biopha.2019.109206

Cited by 35 publications

(32 citation statements)

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“…Remarkably, berberine hydrochloride tablet prepared by BBR was famous in clinic to treat diarrhea in China ( Chen et al, 2015 ). Recently, more and more researchers showed that Phytogenic BBR could treat IBS-D through multiple ways ( Hou et al, 2019 ; Qiong et al, 2019 ). In our study, we verified that BBR could improve diarrhea, visceral hypersensitivity, and depression, anxiety-like behaviors on IBS-D model mice.…”

Section: Discussionmentioning

confidence: 99%

“…Nowadays, it has been shown that CHM are effective in relieving symptoms among patients and rats with IBS-D ( Mao et al, 2017 ; Fan et al, 2020 ). In particular, BBR alkaloid isolated from Coptis chinensis Franch rhizome, which has been widely used for the treatment of diarrhea, exhibit ameliorative effects on rats with IBS-D by modulating BGPs, inhibiting the intestinal inflammatory pathway and regulating the intestinal flora ( Severina et al, 2001 ; Neag et al, 2018 ; Hou et al, 2019 ; Qiong et al, 2019 ). In addition, phytogenic BA, a dominant flavonoid isolated from the roots of Scutellaria baicalensis Georgi, is currently discussed as promising complementary agents in the prevention and treatment of intestinal and neurological diseases by neuroprotective, anti-inflammatory, and regulating intestinal flora ( Ye et al, 2012 ; Zhang et al, 2018 ; Liang et al, 2019 ).…”

Section: Introductionmentioning

confidence: 99%

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Synergistic Effect of Berberine-Based Chinese Medicine Assembled Nanostructures on Diarrhea-Predominant Irritable Bowel Syndrome In Vivo

Cui

et al. 2020

Front. Pharmacol.

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Diarrhea-predominant irritable bowel syndrome (IBS-D) is one common chronic functional disease of the digestive system with limited treatments. The microbiota–gut–brain axis (MGBA) has a central function in the pathogeny of IBS-D, which includes the participation of many various factors, such as brain-gut peptides (BGPs), immune inflammation, and intestinal flora. Inspired by the drug combination in traditional Chinese medicine (TCM), our previous study discovered that berberine (BBR) and baicalin (BA) could form natural self-assemblies as BA-BBR nanoparticles (BA-BBR NPs) and showed synergistic effects against IBS-D. Here, we investigated the synergistic effects of BA-BBR NPs on IBS-D model mice induced by chronic restraint stress plus Senna alexandrina Mill decoction with the influence on MGBA. BA-BBR NPs showed the best therapeutic effect on improving visceral hypersensitivity and diarrhea on IBS-D model mice, compared with BBR, BA, and BA/BBR mixture. Furthermore, BA-BBR NPs significantly ( P <0.05) reduced the levels of 5-hydroxytryptamine (5-HT), vasoactive intestinal polypeptide (VIP) and choline acety transferase (CHAT) in colon tissues or of serum from BGPs; it lowered the expressions of the nuclear factor kappa-B (NF-κB) in colon tissues and changed the levels of basophil granulocyte (BASO) and leukomonocyte (LYMPH) in whole blood from immune inflammation; it altered the intestinal flora of Bacteroidia, Deferribacteres, Verrucomicrobia, Candidatus_Saccharibacteria, and Cyanobacteria from intestinal flora. In conclusion, BA-BBR NPs, after forming the natural self-assembly between BBR and BA, promoted the synergistic effect on IBS-D mice than the sum of BBR and BA effects, based to the formation of self-assemblies rather than the simple mixing. It further proved that synergistic effect of BA-BBR NPs on IBS-D mice might be related to BGPs, immune inflammation, and intestinal flora from three important interrelated components of MGBA. This study will provide a novel idea for the interpretation of TCM compatibility theory and provide the basis for BA-BBR NPs as a medicinal plant-derived natural and efficient nanomaterial for clinical use.

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Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Synergistic Effect of Berberine-Based Chinese Medicine Assembled Nanostructures on Diarrhea-Predominant Irritable Bowel Syndrome In Vivo

Cui

et al. 2020

Front. Pharmacol.

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“…This genus is also rich in pharmacologically active compounds, such as berberine, berbamine, palmatine, oxyacanthine, and jatrorrhizine [52]. Berberine is widely used to treat diarrhea by inhibiting intestinal inflammation and regulating intestinal bacteria [53][54][55][56][57].…”

Section: Important Plant Usesmentioning

confidence: 99%

Wild plants used by the Lhoba people in Douyu Village, characterized by high mountains and valleys, in southeastern Tibet, China

Chen

Yang

et al. 2021

J Ethnobiology Ethnomedicine

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Background Douyu Village, inhabited by the Lhoba people, is situated within the Eastern Himalayas, in southeastern Tibet, China. The village is located among high mountains and valleys, which feature complex terrain with cold and dry climates and distinctive vegetation types and species. The Lhoba people in this village are isolated from other groups in China. The Lhoba people have lived in this village since the 15th century and mainly depended on gathering, hunting, and swidden agriculture before the 1960s. Because they have a long history and live under extreme climatic, geographical, and ecological conditions, the Lhoba people in Douyu Village may have unique traditional knowledge about wild plants. Thus, this research aims to record the traditional botanical knowledge of the Lhoba people in Douyu. Methods An ethnobotanical study was conducted on the Lhoba people in Douyu Village in Longzi County, Tibet, China. Semi-structured interviews and group discussions with informed consent were used in the study. We interviewed 41 informants (14 key informants) between 18 and 75 years of age. All information was collected, organized, and compiled into “use reports” for quantitative analysis. The informant consensus factor (ICF) was used to determine the homogeneity of the informants’ knowledge of medicinal plants, while the cultural importance index (CI) was used to estimate the cultural importance of shared species. Results A total of 91 wild species (90 vascular plants and 1 fungus) belonging to 71 genera and 39 families utilized by the Lhoba people in Douyu were documented. Of these species, Pimpinella xizangense and Wikstroemia lungtzeensis are endemic to Longzi County, while Sinopodophyllum hexandrum and Paeonia ludlowii are endangered species in China. All habitats, from the field vegetation at the valley bottoms to the alpine shrubland and meadows, were used for plant collection, and the numbers of species of plants collected from the various vegetation types (except for fields) decreased with increasing altitude. Our study found that 55 species are edible plants and fungi, 29 species are medicinal plants, and 38 species are used for other purposes. Medicinal plants are used for 11 categories of diseases, among which diseases of blood-forming organs (ICF = 0.96) and gastrointestinal diseases (ICF = 0.95) exhibited the highest ICF values. Based on the CI values, the most important plants in this study area are Berberis xanthophloea, B. kongboensis, Sinopodophyllum hexandrum, Vicatia thibetica, and Hippophae rhamnoides subsp. gyantsensis. Moreover, a comparison of the wild plants used by Lhoba ethnic groups in three counties in China showed significant differences among these regions. Conclusions Our study demonstrates that the wild plants utilized by the Lhoba people in Douyu Village are highly diverse, at 90 plant and one fungal species, which reflects not only the number of species but also their diversified functions. The extreme climatic, geographical, and ecological conditions of Douyu within the high mountains and valleys of the Eastern Himalayas potentially affect the Lhoba people’s culture, including plant utilization practices, and contribute to the rich diversity of the wild plants used by the local people.

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“…Interestingly, a growing number of scholars have paid close attention to the mechanisms of intestinal permeability in recent years. Animal experiments have confirmed that the intestinal permeability of IBS-D mice was significantly increased (Hou et al, 2019). Our previous studies also demonstrated that IBS-D resulted from the permeability enhancement of intestinal mucosa, which might be related to the lower expression of aquaporin (AQP) 1,3,8 (Chao and Zhang, 2018).…”

Section: Introductionmentioning

confidence: 79%

LncRNA H19 as a Competing Endogenous RNA to Regulate AQP Expression in the Intestinal Barrier of IBS-D Patients

et al. 2021

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ObjectiveThe study aimed to investigate the role of Long non-coding RNA (LncRNA) H19 in the pathogenesis of Diarrhea Irritable Bowel Syndrome (IBS-D), and further to the regulatory effect of LncRNA H19 on AQP1, 3 in the intestinal mucosa of IBS-D patients, so as to seek a new way to elucidate the mechanism of IBS in clinic.MethodsThe levels of LncRNA H19, AQP1, and AQP3 were detected in colonic tissues of IBS-D patients, compared with that in healthy controls. Through RNA gene interference and activation methods, small activating RNA (saRNA) and small interfering (siRNA) were transfered into Caco-2 cells in vitro experiment, and sub-group for two control group, siH19 empty vector group, siH19 interference group, overexpression H19 vector group, and overexpression H19 empty vector group. Quantitative real-time reverse transcription-polymerase chain reaction (qRT-PCR) and Western blot were applied to evaluate the expression levels of LncRNA H19 and the amount of AQP1 and AQP3 protein expression, respectively.ResultsCompared with healthy volunteers, the levels of LncRNA H19, AQP1, and AQP3 in the colonic mucosa of IBS-D patients were significantly decreased (P < 0.05). The results in vitro transfection experiment revealed that the level of LncRNA H19 in the siH19 interference group was significantly declined (P < 0.05), while there was a remarkable increase in the overexpression H19 vector group (P < 0.05), compared with the corresponding control groups. The expression of AQP1 and AQP3 in Caco-2 cells was of positive correlation with the level of LncRNA H19.ConclusionThat the down-regulation of LncRNA H19 resulted in the expression changes of AQP1 and AQP3 may play an important role in the occurrence and development of IBS-D.

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Berberine improves intestinal epithelial tight junctions by upregulating A20 expression in IBS-D mice

Cited by 35 publications

References 29 publications

Synergistic Effect of Berberine-Based Chinese Medicine Assembled Nanostructures on Diarrhea-Predominant Irritable Bowel Syndrome In Vivo

Synergistic Effect of Berberine-Based Chinese Medicine Assembled Nanostructures on Diarrhea-Predominant Irritable Bowel Syndrome In Vivo

Wild plants used by the Lhoba people in Douyu Village, characterized by high mountains and valleys, in southeastern Tibet, China

LncRNA H19 as a Competing Endogenous RNA to Regulate AQP Expression in the Intestinal Barrier of IBS-D Patients

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