Berberine enhances survival and axonal regeneration of motoneurons following spinal root avulsion and re-implantation in rats

Xie, Zhaoxiong; Liu, Xiao Dong; Xian, Yan‐Fang; Zhang, Feng; Huang, Pengyun; Tang, Ying; Yuan, Qiuju; Lin, Zhi‐Xiu

doi:10.1016/j.freeradbiomed.2019.08.029

Cited by 22 publications

(18 citation statements)

References 63 publications

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“…Previous study has reported that BBR enhances survival and axonal regeneration of motoneurons following spinal root avulsion and re‐implantation in rats (Zhang et al., 2019); however, whether BBR can regulate axonal remyelination in musculocutaneous nerve to promote motor function recovery and the underlying mechanism remain unclear. Given the key roles of L1 in regeneration and myelination, we were interested in which extent BBR modulates L1 expression in BPRA.…”

Section: Introductionmentioning

confidence: 99%

Berberine enhances L1 expression and axonal remyelination in rats after brachial plexus root avulsion

Chen

Zhou

et al. 2020

Brain and Behavior

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Brachial plexus root avulsion (BPRA), one of brachial plexus injuries, is considered to be a polytrauma associated with motorbikes (Faglioni, Siqueira, Martins, Heise, & Foroni, 2014), especially happen in young men of reproductive age (Giugale, Henrikson, Baronne, & Lee, 2015). In this traumatic condition, extensive motoneuron death in spinal cord, motor axon degeneration in musculocutaneous nerve, and de-innervation of targeted muscles were observed, leading to serious functional deficits in the upper limb (Hallin, Carlstedt, Nilsson-Remahl, & Risling, 1999). Although the injured axons can re-innervate the target muscle after surgical re-implantation of the avulsed ventral roots, the functional recovery is still disappointing (Fang et al., 2016). Several combinatorial strategies have been

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Section: Introductionmentioning

confidence: 99%

Berberine enhances L1 expression and axonal remyelination in rats after brachial plexus root avulsion

Chen

Zhou

et al. 2020

Brain and Behavior

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“…Accumulating evidence has shown that SIRT1 modulates HMGB1 hyperacetylation and inhibits HMGB1 translocation release in different research fields ( Hwang et al., 2015 ; Chen X. et al., 2018 ). Intriguingly, recent studies in other research areas has proved that berberine is a potent SIRT1 activator ( Sun et al., 2018 ; Zhang et al., 2019a ). In agreement with previous studies, our results showed that berberine could significantly upregulate SIRT1 expression and inhibit HMGB1/Nf-κB activation to reduce brain injury after SAH.…”

Section: Discussionmentioning

confidence: 99%

“…Berberine is a well-known natural compound that possess a variety of biological functions. In the CNS, studies have shown that berberine could easily pass the BBB and provide beneficial effects against a variety of neurological disorders (Liu D. Q. et al, 2019;Zhang et al, 2019a). But no study has yet investigated the potential effects in experimental SAH.…”

Section: Discussionmentioning

confidence: 99%

“…In addition, berberine is a small molecule and can easily penetrate the blood-brain barrier (BBB). Intriguingly, recent studies have reported that berberine is a potent SIRT1 agonist and is able to inhibit HMGB1/Nf-κB pathway in a variety of disease models ( Sun et al., 2018 ; Zhang et al., 2019a ; Zhou et al., 2020 ). However, no study has performed to investigate the potential role of berberine in experimental SAH.…”

Section: Introductionmentioning

confidence: 99%

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Berberine Ameliorates Subarachnoid Hemorrhage Injury via Induction of Sirtuin 1 and Inhibiting HMGB1/Nf-κB Pathway

et al. 2020

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Excessive cerebral inflammation plays a key role in early brain injury (EBI) after subarachnoid hemorrhage (SAH). Berberine, an isoquinoline alkaloid isolated from Chinese herb Coptis chinensis, possesses anti-inflammatory, and neuroprotective effects. Here we evaluated the beneficial effects of berberine against SAH-induced inflammatory response and the subsequent brain injury. Our data showed that berberine treatment significantly inhibited microglia activation and proinflammatory cytokines release. Concomitant with suppressed cerebral inflammation, berberine mitigated the subsequent brain injury as demonstrated by improved neurological behavior, reduced brain edema, and decreased neural apoptosis. Moreover, berberine significantly inhibited high mobile group box 1 (HMGB1)/nuclear factor-kB (Nf-kB)dependent pathway and enhanced sirtuin 1 (SIRT1) expression after SAH. Treatment with ex527, a selective SIRT1 inhibitor, reversed berberine-induced SIRT1 activation and inhibitory effects on HMGB1/Nf-kB activation. In addition, ex527 pretreatment abated the anti-inflammatory and neuroprotective effects of berberine on SAH. Taken together, these findings suggest that berberine provides beneficial effects against SAH-triggered cerebral inflammation by inhibiting HMGB1/Nf-kB pathway, which may be modulated by SIRT1 activation.

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“…Neuroin ammation is one of the essential factors for triggering the neuron loss related to acute or chronic neurodegenerative disorders [3]. It has been well documented that the neuroin ammation also plays a causal role for the MNs degeneration and death associated with spinal cord injury, including avulsion injury [4][5] [6] [7].…”

Section: Introductionmentioning

confidence: 99%

Cytokine Profile and Glial Activation Following Brachial Plexus Roots Avulsion Injury in Mice

Zhong

Tang

et al. 2020

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Background Inflammation and tissue infiltration by various immune cells play a significant role in the pathogenesis of neurons suffering the central nervous systems diseases. Although brachial plexus root avulsion (BPRA) leads to dramatic motoneurons (MNs) death and permanent loss of function, however, the crosstalk between cytokines and glial reaction in the spinal cord during injury is far beyond our knowledge. The current study is sought to investigate the alteration of specific cytokine expression patterns of BPRA injured spinal cord during an acute and subacute period.Methods Here, cytokine assay, transmission electron microscopy, and histological staining were utilized to assess the alteration of cytokine network, ultrastructure morphology, as well as glial activation and loss of MNs within two weeks, post-injury on a mouse BPRA model.Results We found that BPRA significantly changed the level of CXCL1, IL12 p70, ICAM1, IP10, MCP-5, MIP1-α and CD93. Moreover, the elevated MIP1-α and CD93 were mostly distributed in MNs of the injured, but few in healthy segments. Also, BPRA significantly induced glial reactions in the ventral horn of injured spinal segments, reflected by robustly elevated the Ionized calcium-binding adaptor molecule 1 (IBA1) and Glial fibrillary acidic protein (GFAP) positive cells. We also observed a severe progressively loss of injured MNs, with a character of both apoptosis and necrosis.Conclusion Overall, these findings suggested that the inflammatory cytokines associated with glial cell proliferation play a vital role in the pathophysiology of MNs death caused by nerve roots injury.

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Berberine enhances survival and axonal regeneration of motoneurons following spinal root avulsion and re-implantation in rats

Cited by 22 publications

References 63 publications

Berberine enhances L1 expression and axonal remyelination in rats after brachial plexus root avulsion

Berberine enhances L1 expression and axonal remyelination in rats after brachial plexus root avulsion

Berberine Ameliorates Subarachnoid Hemorrhage Injury via Induction of Sirtuin 1 and Inhibiting HMGB1/Nf-κB Pathway

Cytokine Profile and Glial Activation Following Brachial Plexus Roots Avulsion Injury in Mice

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